Onion yellow dwarf virus ∆∆Ct-based relative quantification obtained by using real-time polymerase chain reaction in ‘Rossa di Tropea’ onion

Tiberini, Antonio; Mangano, Rossella; Micali, Giuseppe; Leo, Giovanna; Manglli, Ariana; Tomassoli, L.; Albanese, G.

doi:10.1007/s10658-018-1560-2

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…The effectiveness of OYDV inoculation was verified as reported by Taglienti et al [ 37 ], by an OYDV-specific serological test (ELISA) (BIOREBA, Reinach, Switzerland), following the manufacturer’s instructions. Inoculated plants negative at the ELISA test were further tested by quantitative real-time PCR (qPCR) to verify the infection efficiency [ 38 ]. Assays were performed in triplicate, including negative (uninfected plants and water) and positive (OYDV-infected plants by ELISA) controls.…”

Section: Methodsmentioning

confidence: 99%

WRKY Gene Family Drives Dormancy Release in Onion Bulbs

Puccio

Crucitti

Tiberini

et al. 2022

Cells

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Onion (Allium cepa L.) is an important bulb crop grown worldwide. Dormancy in bulbous plants is an important physiological state mainly regulated by a complex gene network that determines a stop of vegetative growth during unfavorable seasons. Limited knowledge on the molecular mechanisms that regulate dormancy in onion were available until now. Here, a comparison between uninfected and onion yellow dwarf virus (OYDV)-infected onion bulbs highlighted an altered dormancy in the virus-infected plants, causing several symptoms, such as leaf striping, growth reduction, early bulb sprouting and rooting, as well as a lower abscisic acid (ABA) level at the start of dormancy. Furthermore, by comparing three dormancy stages, almost five thousand four hundred (5390) differentially expressed genes (DEGs) were found in uninfected bulbs, while the number of DEGs was significantly reduced (1322) in OYDV-infected bulbs. Genes involved in cell wall modification, proteolysis, and hormone signaling, such as ABA, gibberellins (GAs), indole-3-acetic acid (IAA), and brassinosteroids (BRs), that have already been reported as key dormancy-related pathways, were the most enriched ones in the healthy plants. Interestingly, several transcription factors (TFs) were up-regulated in the uninfected bulbs, among them three genes belonging to the WRKY family, for the first time characterized in onion, were identified during dormancy release. The involvement of specific WRKY genes in breaking dormancy in onion was confirmed by GO enrichment and network analysis, highlighting a correlation between AcWRKY32 and genes driving plant development, cell wall modification, and division via gibberellin and auxin homeostasis, two key processes in dormancy release. Overall, we present, for the first time, a detailed molecular analysis of the dormancy process, a description of the WRKY-TF family in onion, providing a better understanding of the role played by AcWRKY32 in the bulb dormancy release. The TF co-expressed genes may represent targets for controlling the early sprouting in onion, laying the foundations for novel breeding programs to improve shelf life and reduce postharvest.

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Section: Methodsmentioning

confidence: 99%

WRKY Gene Family Drives Dormancy Release in Onion Bulbs

Puccio

Crucitti

Tiberini

et al. 2022

Cells

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“…Mechanical inoculation was performed through 10 needle punctures along two different leaves for each plant to simulate the probing activity of aphid vectors. Before each puncture, the syringe needle was soaked in a sap of OYDV-infected leaves (OYDV-5 strain provided by the Department of Agraria, University Mediterranea of Reggio Calabria, previously characterized [21]), extracted in presence of phosphate buffer (0.1 M; 1:5 w:v). At 30 days post inoculation (dpi) both inoculated and not-inoculated plants were analyzed by DAS-ELISA [22] using a commercial kit (Bioreba Reinach, Reinach, Switzerland) and following the manufacturer's instructions.…”

Section: Germplasm Collection Originmentioning

confidence: 99%

Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection

Corrado,

Micali,

Mauceri

et al. 2024

Horticulturae

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The onion yellow dwarf virus (OYDV) represents a limiting biotic stress in onion (Allium cepa L.); little information is available regarding resistant varieties. In Italy, onion production is limited but represented by a wide diversity of ecotypes. A two-year trial was carried out to test the OYDV-susceptibility/tolerance of different Italian onion cultivars by rating symptom severity and plant growth parameters and assessing post-harvest secondary infections. The cultivar and ecotypes included in the study were characterized by simple sequence repeats (SSR) analysis, and the expression analysis of two genes (Eukaryotic translation initiation factors, EIFs) involved in potyvirus replication was also performed. Two susceptible and one tolerant cultivar were identified based on symptom expression and virus impact on plants. Although differences in growth parameters were limited to the first-year trial, the infection was correlated to a higher incidence of secondary infections in post-harvest, with altered water balance in infected bulbs. This correlation was also demonstrated during the long-term storage of bulbs. SSR analysis identified different clusters and only one gene isoform (EIF4eiso1) showed different expression levels in the OYDV/onion pathosystem. In conclusion, this study defines the genetic profile of Italian onion cultivars and provides evidence on susceptibility/tolerance features which will be useful in the future for the identification of viral resistance traits in onion.

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“…Plant material (i.e., onion bulbs) was obtained from an experimental trial established as follows. Seeds of 'Rossa di Tropea' onion (biotype locally named Mezza campana), collected from healthy plants as assessed by specific OYDV Real-Time RT-PCR test [33], were used as starting material. All the procedures performed during the different trial phases were carried out in an insect-proof screenhouse at "Mediterranea" University of Reggio Calabria, Italy.…”

Section: Plant Materials and Experimental Setupmentioning

confidence: 99%

“…To ascertain sanitary status of infected (i) and healthy (h) plants after inoculation, leaf samples collected from all the 180 plants in the experimental trial were assayed by DAS-ELISA (BIOREBA, Reinach, Switzerland) and RealTime RT-PCR [33]. The sampling and analyses were performed 21 days post inoculum (d.p.i.).…”

Section: Phytosanitary Status Assessmentmentioning

confidence: 99%

Characterization of Volatile Organic Compounds in ‘Rossa di Tropea’ Onion by Means of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry (HS/SPME GC–MS) and Sensory Analysis

et al. 2021

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Background: Plant viral infections induce changes in the host plant, which can potentially impact composition, organoleptic properties, and storability characteristics of plant products. In particular, onion odor and flavor are determined mainly by volatile organic compounds, and changes upon infection with onion yellow dwarf virus may deeply influence these characters. Methods: A time-course study of volatile organic compounds in onion yellow dwarf virus-infected versus healthy ‘Rossa di Tropea’ onion bulbs was performed using headspace solid-phase microextraction gas chromatography–mass spectrometry; sensory analysis performed at marketability stage of onion production was used to correlate such changes to the taste characteristics perceived by consumers. Results: Volatile organic compounds regulated in infection conditions were identified, mainly belonging to mono- and poly-sulfides classes. The most abundant compounds in the analyzed samples were propyl disulfide, allyl-isopropyl disulfide, and propanethiol; significantly different concentrations were observed for 7 out of 11 VOCs in virus-infected compared to healthy bulbs. Statistical analysis based on a partial least squares discriminant analysis model and hierarchical cluster analysis allowed us to cluster samples based on phytosanitary status and storage time and to identify the most responsible compounds for such classification. Conclusions: Onion yellow dwarf virus infection induces changes in volatile organic compounds in onion during storage. The impact of such regulated compounds on ‘Rossa di Tropea’ onion odor and flavor and correlation with sensory analysis are discussed.

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Onion yellow dwarf virus ∆∆Ct-based relative quantification obtained by using real-time polymerase chain reaction in ‘Rossa di Tropea’ onion

Cited by 8 publications

References 30 publications

WRKY Gene Family Drives Dormancy Release in Onion Bulbs

WRKY Gene Family Drives Dormancy Release in Onion Bulbs

Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection

Characterization of Volatile Organic Compounds in ‘Rossa di Tropea’ Onion by Means of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry (HS/SPME GC–MS) and Sensory Analysis

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