2023
DOI: 10.1007/978-1-0716-2843-0_23
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Onion (Allium cepa L.) Pollen Cryopreservation Protocol

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“…In recent years, the efficiency of onion germplasm management has seen a greater improvement, especially following the development of new methods such as cryopreservation of Alliums’ genetic resources [ 82 ]. Cryopreservation represents an advanced ex situ preservation method for long-term storage of germplasm, involving freezing at ultra-low temperatures (−196 °C) in liquid nitrogen media [ 9 , 78 , 83 ]. This technique has contributed to increased effectiveness in the management of many crop accessions stored in various genebanks, providing a cheaper approach toenhancing germplasm health and meticulous characterization and evaluation of accessions [ 51 ].…”
Section: Ex Situ Conservation Efforts For Onion Genetic Resourcesmentioning
confidence: 99%
“…In recent years, the efficiency of onion germplasm management has seen a greater improvement, especially following the development of new methods such as cryopreservation of Alliums’ genetic resources [ 82 ]. Cryopreservation represents an advanced ex situ preservation method for long-term storage of germplasm, involving freezing at ultra-low temperatures (−196 °C) in liquid nitrogen media [ 9 , 78 , 83 ]. This technique has contributed to increased effectiveness in the management of many crop accessions stored in various genebanks, providing a cheaper approach toenhancing germplasm health and meticulous characterization and evaluation of accessions [ 51 ].…”
Section: Ex Situ Conservation Efforts For Onion Genetic Resourcesmentioning
confidence: 99%