One year outcome of hemi-Descemet membrane endothelial keratoplasty

Lam, Fook Chang; Baydoun, Lamis; Satué, María; Dirisamer, Martin; Ham, Lisanne; Melles, Gerrit R.J.

doi:10.1007/s00417-015-3097-9

Cited by 20 publications

(13 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the scarcity of donor tissue led to endeavours to save donor tissue, for example, by splitting tissue into two parts for so-called hemi-DMEKs (Lam et al 2014(Lam et al , 2015Gerber-Hollbach et al 2016). This seems to be associated with an equally good gain of transparency but a significant drop of ECD postoperatively.…”

Section: Worldwidementioning

confidence: 99%

See 1 more Smart Citation

Impact of donor tissue diameter on postoperative central endothelial cell density in Descemet Membrane Endothelial Keratoplasty

Schrittenlocher

Bachmann

Cursiefen

2018

Acta Ophthalmologica

View full text Add to dashboard Cite

Purpose To assess the effect of donor tissue diameter in Descemet Membrane Endothelial Keratoplasty (DMEK) on postoperative endothelial cell density (ECD) mainly in Fuchs Endothelial Corneal Dystrophy (FECD) patients. Methods Retrospective review of 693 consecutive DMEK surgeries from the prospective Cologne DMEK database performed between 07/2011 and 07/2016 at the Department of Ophthalmology, University of Cologne. Eight‐ versus ten‐millimetre large donor DMEK grafts of two different surgeons using identical surgical techniques were compared. Results A total of 693 consecutive DMEK surgeries were included. Two groups (8 versus 10 mm DMEK graft diameter) were compared: 23% of eyes received 8‐mm grafts (group 1, n = 160) and 77% 10‐mm grafts (group 2, n = 533). Mean preoperative ECD (±SD) of donor tissue was 2736 ± 224 cells/mm2 in group 1 and 2714 ± 232 in group 2. Group 1 showed a mean ECD of 1740 ± 439 cells/mm2 (mean ± SD; n = 67) 12 months after DMEK compared to 1664 ± 404 cells/mm2 (mean ± SD; n = 344) in group 2. The difference between the 6‐ and 12‐month ECD of both groups was not significant (p = 0.675; p = 0.161). Total decrease in ECD 6‐ and 12‐months postoperatively was 38 ± 14 (n = 128) and 36 ± 16% in group 1 (n = 66) versus 37 ± 15% (n = 398) and 38 ± 15% in group 2 (n = 342; p = 0.414; p = 0.198, respectively). Conclusion Our data suggest that postoperative central endothelial cell density is not significantly associated with DMEK graft diameters in the range of 8–10 mm in cases with healthy peripheral host endothelium (such as FECD).

show abstract

Section: Worldwidementioning

confidence: 99%

“…Furthermore, the scarcity of donor tissue led to endeavours to save donor tissue, for example, by splitting tissue into two parts for so‐called hemi‐DMEKs (Lam et al. , ; Gerber‐Hollbach et al. ).…”

Section: Introductionmentioning

confidence: 99%

Impact of donor tissue diameter on postoperative central endothelial cell density in Descemet Membrane Endothelial Keratoplasty

Schrittenlocher

Bachmann

Cursiefen

2018

Acta Ophthalmologica

View full text Add to dashboard Cite

show abstract

“…Our results of alizarin red staining displayed that the ECD of the TE-HCE-transplanted rabbit corneas was 2703 ± 70.37 cells/mm 2 on average 358 days after PKP surgery, while that of control corneas was only 2509.90 ± 51.11 cells/mm 2 . The ECD of the TE-HCE-transplanted rabbit cornea was higher than that of the corneas in TE-HCE-transplanted rabbits and cats in our previous study (Fan TJ et al, 2011;Fan et al, 2013), and much higher than that of the corneas 12 months after endothelial keratoplasty in donated cornea-transplanted patients (<2000 cells/ mm 2 ) (Van Dooren et al, 2004;Kettesy et al, 2014;Lam et al, 2015;Yoeruek et al, 2015). Moreover, our results of H&E staining and SEM and TEM studies revealed that a continuous monolayer of corneal endothelial cells in hexagonal or polygonal morphology attached tightly to the de novo generated DM was reconstructed.…”

Section: Discussionmentioning

confidence: 55%

Construction of a tissue-engineered human corneal endothelium and its transplantation in rabbit models

Zhao¹,

Ma²,

Fan³

2016

Turk J Biol

View full text Add to dashboard Cite

Human corneal endothelium (HCE) is vital in maintaining corneal transparency and thickness, and damages to it may result in endotheliopathy and even corneal blindness, which can only be cured by keratoplasty. Due to the shortage of donor corneas, tissueengineered HCE (TE-HCE) has become an equivalent to HCE. This study constructs a high endothelial cell density (ECD) TE-HCE and evaluates its functions by corneal transplantation in rabbits. A TE-HCE was constructed by culturing DiI-labeled nontransfected HCE cells on modified denuded amniotic membrane (mdAM) using collagen IV, fibronectin, and laminin, and rabbit corneas were characterized both in vivo and ex vivo after TE-HCE transplantation. Our results indicated that the constructed TE-HCE with a high ECD of 3611 ± 56.66 cells/mm2 had similar morphology and structure to a native HCE. In vivo postsurgery detections showed that the TE-HCE-transplanted cornea gained transparency and recovered its thickness gradually during a monitoring period of 358 days, while the mdAM-transplanted cornea remained opaque and edematous. Ex vivo examinations revealed that a native-like corneal endothelium with an ECD of 2703 ± 70.37 cells/mm2 was reconstructed by the transplanted TE-HCE. Our findings suggested that the TE-HCE might be used as a HCE equivalent and has promising applications in therapy of corneal endotheliopathy.

show abstract

“…Although some mechanical damage was observed at the cut edge, as well as at the very limbal edge, the endothelial layer appeared viable up to 5 weeks in organ culture (after which, deterioration of the cellular morphology was seen similar to that in routine culture of full-thickness corneas). Preliminary surgical experience showed that routine DMEK techniques can be used for hemi-DMEK grafts 17 18. In this initial small series of the first consecutive three cases, the visual outcomes of hemi-DMEK appeared to be similar to DMEK, but the endothelial cell decrease with hemi-DMEK was larger,17 18 though a much larger clinical case series would be required for more definitive results.…”

Section: Discussionmentioning

confidence: 88%

“…Preliminary surgical experience showed that routine DMEK techniques can be used for hemi-DMEK grafts 17 18. In this initial small series of the first consecutive three cases, the visual outcomes of hemi-DMEK appeared to be similar to DMEK, but the endothelial cell decrease with hemi-DMEK was larger,17 18 though a much larger clinical case series would be required for more definitive results. Another current limitation of this technique that might need consideration in the clinical practice is the shape mismatch of the hemi-DMEK graft and the area of the circular descemetorhexis.…”

Section: Discussionmentioning

confidence: 88%

Graft preparation for hemi-Descemet membrane endothelial keratoplasty (hemi-DMEK)

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

One year outcome of hemi-Descemet membrane endothelial keratoplasty

Cited by 20 publications

References 9 publications

Impact of donor tissue diameter on postoperative central endothelial cell density in Descemet Membrane Endothelial Keratoplasty

Impact of donor tissue diameter on postoperative central endothelial cell density in Descemet Membrane Endothelial Keratoplasty

Construction of a tissue-engineered human corneal endothelium and its transplantation in rabbit models

Graft preparation for hemi-Descemet membrane endothelial keratoplasty (hemi-DMEK)

Contact Info

Product

Resources

About