2013
DOI: 10.1016/j.joen.2013.04.027
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One-Visit Versus Two-Visit Root Canal Treatment: Effectiveness in the Removal of Endotoxins and Cultivable Bacteria

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Cited by 70 publications
(127 citation statements)
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“…Determination of endotoxin concentration (kinetic chromogenic LAL assay) The kinetic chromogenic LAL assay (Lonza) used for quantification of endotoxins had been previously reported by the author [24]. Briefly, for the test, 100-mL apyrogenic water (reaction blank), the five standard endotoxin solutions (0.005-50 endotoxin units [EU]/mL), root canal samples, and positive controls (each root canal sample contaminated with a known concentration of endotoxin [10 EU/ mL]) were added to a 96-well apyrogenic plate.…”
Section: Methodsmentioning
confidence: 99%
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“…Determination of endotoxin concentration (kinetic chromogenic LAL assay) The kinetic chromogenic LAL assay (Lonza) used for quantification of endotoxins had been previously reported by the author [24]. Briefly, for the test, 100-mL apyrogenic water (reaction blank), the five standard endotoxin solutions (0.005-50 endotoxin units [EU]/mL), root canal samples, and positive controls (each root canal sample contaminated with a known concentration of endotoxin [10 EU/ mL]) were added to a 96-well apyrogenic plate.…”
Section: Methodsmentioning
confidence: 99%
“…Determination of cultivable bacterial counts (culturing procedure) The method used for culture procedures in the present study had been previously reported by the author [24]. Briefly, the transport media containing the root canal samples were thoroughly shaken for 60 s (Vortex; Marconi, Piracicaba, São Paulo, Brazil).…”
Section: Methodsmentioning
confidence: 99%
“…The fundamental and clinical research on single-visit root canal treatment has been widely applied as early as 19th century [4]. However, the efficacy of these treatments is inconclusive [5].…”
Section: Discussionmentioning
confidence: 99%
“…Contudo, nem o tratamento em sessão única nem o de sessão múltipla podem eliminar completamente as colônias microbianas 17 . Em relação à redução de endotoxinas, o tratamento em sessão única mostrou-se menos eficaz do que em sessões múltiplas 18 . Os fatores a serem considerados na escolha da modalidade de tratamento endodôntico são a habilidade do operador, a experiência clínica, as condições do dente (dente vital ou não vital, sintomático ou assintomático, presença ou ausência de exsudato e edema), o adequado tempo de tratamento, as limitações de tempo do paciente, a história médica e as considerações anatômicas e biológicas.…”
Section: Revisão De Literaturaunclassified