One type of VEGFR is involved in WSSV infection to the Pacific whiteleg shrimp Litopenaeus vannamei

Li, Shihao; Wang, Zhiwei; Li, Fuhua; Xiang, Jianhai

doi:10.1016/j.dci.2015.01.001

Cited by 19 publications

(15 citation statements)

References 43 publications

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“…The data indicated that LvVEGFR1 might be an early response gene to WSSV infection, while LvVEGFR2 might be a late response gene. It should be also noted that there was a significant decrease of LvVEGFR2 expression at 1 hpi in Oka, which was similar to the decrease of LvVEGFR1 at 1 hpi in intestine ( 16 ). A possible reason for the decreases might be that WSSV infection recruited VEGFs from other tissues into hemolymph since WSSV would first enter the hemolymph after muscle injection.…”

Section: Discussionsupporting

confidence: 65%

“…At 24 h post WSSV infection, the expression level of LvVEGFR2 gene in hemocytes and Oka was significantly upregulated. Different from LvVEGFR2 , the previously reported LvVEGFR1 gene was upregulated at 1 h post WSSV infection in hemocytes ( 16 ). The data indicated that LvVEGFR1 might be an early response gene to WSSV infection, while LvVEGFR2 might be a late response gene.…”

Section: Discussionmentioning

confidence: 79%

“…In crustacean, VEGF signaling pathway participates in innate immunity and is responsive to pathogen infection ( 13 , 14 ). Previously, we characterized a VEGFR gene ( LvVEGFR1 ) and three VEGF genes in L. vannamei and data showed that they were all involved in WSSV infection ( 16 – 18 ). Here, we identified a new VEGFR gene, LvVEGFR2 , in L. vannamei and characterized its function during WSSV infection.…”

Section: Discussionmentioning

confidence: 99%

“…Interference of LvVEGFR1 gene by double-strand RNA could inhibit the in vivo propagation of WSSV in shrimp ( 16 ). Silencing of other members in shrimp VEGF signaling pathway, including LvVEGF1, LvVEGF2 , and LvVEGF3 genes, could also reduce WSSV copy number in WSSV-infected shrimp ( 17 , 18 ).…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, we isolated three VEGF genes ( LvVEGF1, LvVEGF2 , and LvVEGF3 ) and a receptor gene LvVEGFR from L. vannamei . Silencing of these genes by dsRNA-mediated RNAi could reduce the in vivo copy number of WSSV and the accumulative mortality rate of the infected shrimp decreased apparently ( 16 – 18 ). These data suggested that the VEGF and VEGFR genes played important roles in the host immunity.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Vascular Endothelial Growth Factor Receptor Participates in White Spot Syndrome Virus Infection in Litopenaeus vannamei

Wang

et al. 2017

Front. Immunol.

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Vascular endothelial growth factor (VEGF) signaling pathway is known to play key roles in endothelial cell proliferation, migration, angiogenesis, vascular permeability, inhibition of apoptosis, and virus infection. In the present study, a novel VEGFR gene (LvVEGFR2) was identified and characterized from Litopenaeus vannamei. The deduced amino acid sequence of LvVEGFR2 possessed typical features of VEGFRs reported in other species, including six IG-like domains, a transmembrane motif, a protein kinase (PK) domain, and one tyrosine-PK active site. The transcripts of LvVEGFR2 were mainly detected in hemocytes and lymphoid organ (Oka). Subcellular localization analysis showed that LvVEGFR2 was a membrane protein. Its expression level was obviously upregulated in hemocytes and Oka of the shrimp after white spot syndrome virus (WSSV) infection. Knockdown of LvVEGFR2 gene expression by double-strand RNA mediated interference could lead to a decrease of virus copy number in WSSV-infected shrimp. The interaction between LvVEGFR2 and different LvVEGFs (LvVEGF1, LvVEGF2, and LvVEGF3) in shrimp was analyzed at the transcription level and protein level, respectively. Knockdown of LvVEGF2 or LvVEGF3 could downregulate the expression level of LvVEGFR2, and injection of the recombinant LvVEGF2 or LvVEGF3 could upregulate the expression level of LvVEGFR2. Yeast two-hybrid analysis showed that LvVEGFR2 could interact with LvVEGF2 and LvVEGF3 directly. The study improved our understanding on the VEGF signaling pathway of shrimp and its role during WSSV infection.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 79%