One Type of Bio-Phase Microscopy Imaging Method Based on a Con Beam with Double Dove Prisms

Abstract

Abstract. Optical interference is an important technique in precision measurement and microscopy imaging. Especially, phase imaging technology of the biological cell has attracted increasing attention. In this paper, considering the high stability characteristics of the coaxial interference technique, one interference imaging system that can do both coaxial and off-axis phase imaging is constructed by using a couple of Dove prisms and a beam splitter without other lens, all the system structure based on a con beam that is simple and can image the phase of a cell after placing a post amplification. It can be obtained by some phase recovery methods for the thickness distribution of a cell, so that, the shape of a homogeneous cell can be shown. Simulation and experimental results have verified the feasibility and correctness of the system. It is one kind of cell morphology analysis technology with simple and practical.