“One-to-three” droplet generation in digital microfluidics for parallel chemiluminescence immunoassays

Jin, Kai; Hu, Chin-Hwa; Hu, Siyi; Hu, Chengyou; Li, Jinhua; Ma, Hanbin

doi:10.1039/d1lc00421b

Cited by 50 publications

(43 citation statements)

References 34 publications

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“…Its principle is to use the difference in the surface tension to control the droplet, and the core technology is how to efficiently and stably generate microdroplets. Recently, Ma's ( Jin et al, 2021 ) group at the Suzhou Institute of Biomedical Engineering and Technology at the Chinese Academy of Sciences proposed a digital microfluidic chip that can divide a large droplet into three small droplets. The smaller droplet size can break the minimum volume limit.…”

Section: Some Recent Research On Microfluidic Chipsmentioning

confidence: 99%

Microfluidic technology and its application in the point-of-care testing field

Xie

Dai

Yang

2022

Biosensors and Bioelectronics: X

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Since the outbreak of the coronavirus disease 2019 (COVID-19), countries around the world have suffered heavy losses of life and property. The global pandemic poses a challenge to the global public health system, and public health organizations around the world are actively looking for ways to quickly and efficiently screen for viruses. Point-of-care testing (POCT), as a fast, portable, and instant detection method, is of great significance in infectious disease detection, disease screening, pre-disease prevention, postoperative treatment, and other fields. Microfluidic technology is a comprehensive technology that involves various interdisciplinary disciplines. It is also known as a lab-on-a-chip (LOC), and can concentrate biological and chemical experiments in traditional laboratories on a chip of several square centimeters with high integration. Therefore, microfluidic devices have become the primary implementation platform of POCT technology. POCT devices based on microfluidic technology combine the advantages of both POCT and microfluids, and are expected to shine in the biomedical field. This review introduces microfluidic technology and its applications in combination with other technologies.

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Section: Some Recent Research On Microfluidic Chipsmentioning

confidence: 99%

Microfluidic technology and its application in the point-of-care testing field

Xie

Dai

Yang

2022

Biosensors and Bioelectronics: X

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“…For example, electrodes of different sizes induce unequal droplet splitting that produces daughter droplets of different volumes, and stripped electrodes of rectangular shape enable parallel microdroplet generation . Satellite droplets are formed during droplet splitting, , which can be harnessed for generating submicroliter droplets that break down the geometry limit imposed by electrodes . Besides, digital microfluidics demonstrates the ability to produce two-phase droplets composed of an aqueous and an organic compartment, which is useful for acid–base workup, liquid–liquid extraction, and in-line solvent-swap …”

Section: Microfluidics-enabled Soft Manufacture Of Materialsmentioning

confidence: 99%

Microfluidics-Enabled Soft Manufacture of Materials with Tailorable Wettability

Zhu

Wang

2021

Chem. Rev.

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Microfluidics and wettability are interrelated and mutually reinforcing fields, experiencing synergistic growth. Surface wettability is paramount in regulating microfluidic flows for processing and manipulating fluids at the microscale. Microfluidics, in turn, has emerged as a versatile platform for tailoring the wettability of materials. We present a critical review on the microfluidics-enabled soft manufacture (MESM) of materials with well-controlled wettability and their multidisciplinary applications. Microfluidics provides a variety of liquid templates for engineering materials with exquisite composition and morphology, laying the foundation for precisely controlling the wettability. Depending on the degree of ordering, liquid templates are divided into individual droplets, one-dimensional (1D) arrays, and two-dimensional (2D) or three-dimensional (3D) assemblies for the modular fabrication of microparticles, microfibers, and monolithic porous materials, respectively. Future exploration of MESM will enrich the diversity of chemical composition and physical structure for wettability control and thus markedly broaden the application horizons across engineering, physics, chemistry, biology, and medicine. This review aims to systematize this emerging yet robust technology, with the hope of aiding the realization of its full potential.

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“…The droplet volumes can be extremely small, ranging from a few nL to fL, 30 saving chemicals, 30 reducing reaction times, 30 and allowing massively parallel operation. 31 Typically, the droplets are water-based in an oil medium, and the droplets are produced with a flow-focusing device or a T-junction, 18 , 32 where an immiscible continuous flow in a surrounding medium flow breaks into droplets. Droplets are a versatile medium for encapsulating and transporting cells, bacteria, or drugs, contributing to scaling down laboratory processes into miniaturized laboratories-on-chips, with studies ranging from genome sequencing, 33 polymerase chain reactions, 34 studying micro-organisms, 35 high-throughput screening, 36 , 37 and directed evolution.…”

Section: Introductionmentioning

confidence: 99%

“…Using immiscible droplets in an inert medium as tiny carriers of samples and reagents is the goal of droplet microfluidics. The droplet volumes can be extremely small, ranging from a few nL to fL, saving chemicals, reducing reaction times, and allowing massively parallel operation . Typically, the droplets are water-based in an oil medium, and the droplets are produced with a flow-focusing device or a T-junction, , where an immiscible continuous flow in a surrounding medium flow breaks into droplets.…”

Section: Introductionmentioning

confidence: 99%

Programmable Droplet Microfluidics Based on Machine Learning and Acoustic Manipulation

2022

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Typical microfluidic devices are application-specific and have to be carefully designed to implement the necessary functionalities for the targeted application. Programmable microfluidic chips try to overcome this by offering reconfigurable functionalities, allowing the same chip to be used in multiple different applications. In this work, we demonstrate a programmable microfluidic chip for the two-dimensional manipulation of droplets, based on ultrasonic bulk acoustic waves and a closed-loop machine-learning-based control algorithm. The algorithm has no prior knowledge of the acoustic fields but learns to control the droplets on the fly. The manipulation is based on switching the frequency of a single ultrasonic transducer. Using this method, we demonstrate 2D transportation and merging of water droplets in oil and oil droplets in water, and we performed the chemistry that underlies the basis of a colorimetric glucose assay. We show that we can manipulate drops with volumes ranging from ∼200 pL up to ∼30 nL with our setup. We also demonstrate that our method is robust, by changing the system parameters and showing that the machine learning algorithm can still complete the manipulation tasks. In short, our method uses ultrasonics to flexibly manipulate droplets, enabling programmable droplet microfluidic devices.

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“One-to-three” droplet generation in digital microfluidics for parallel chemiluminescence immunoassays

Abstract: In digital microfluidics, droplet generation is a fundamental operation for quantitative liquid manipulation. The generation of well-defined micro-droplets on a chip with restricted device geometries has become a real obstacle...

Cited by 50 publications

References 34 publications

Microfluidic technology and its application in the point-of-care testing field

Microfluidic technology and its application in the point-of-care testing field

Microfluidics-Enabled Soft Manufacture of Materials with Tailorable Wettability

Programmable Droplet Microfluidics Based on Machine Learning and Acoustic Manipulation

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