One-step transformation of two Andean potato cultivars (Solanum tuberosum L. subsp. andigena)

Trujillo, C.; Rodriguez-Arango, Esperanza; Jaramillo, Sonia; Sánchez, Rodrigo Alberto Hoyos; Ordúz, Sergio; Arango, Rafael

doi:10.1007/s002990100381

Cited by 28 publications

(17 citation statements)

References 9 publications

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“…Similar transformation efficiencies have been reported by Beaujean et al 1998, Cingel et al 2010, Borna et al 2010, Chakravarty and Wang-Prusky 2010 Incidentally, like the present study these authors used the tuberosum subspecies. In contrast, low regeneration and transformation rates were reported in andigena species (Trujillo et al 2001, Lopéz and Chaparro, 2007, Valderama et al 2007. These investigators used a one-step transformation protocol compared to a two-step reported in the present study.…”

Section: Resultsmentioning

confidence: 94%

“…< ronamu2003@yahoo.co.uk>. organisms has provided promising results in improving the yield and quality of potato cultivars (Wierenga et al 1996, Hefferon et al 1997, Jeong et al 2001, Trujillo et al 2001, Lyapkova et al 2001, Naimov et al 2001, Urwin et al 2001, Chue et al 2004. Establishment of an efficient in vitro regeneration protocol is a prerequisite to facilitate successful plant genetic transformation (Soto et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

Onamu

Legaria

Sahagún

et al. 2013

Plant Tissue Cult. & Biotech.

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Prior to Agrobacterium-mediated genetic transformation in vitro regeneration protocol was established for three potato cultivars (Alfa, Cambray Rosa Morelos and Atlantic) grown in Mexico using leaf, node and internodal explants. Regeneration protocol was developed with or without the intervention of callus. Two potato cultivars, namely, Cambray Rosa Morelos and Alpha were transformed using Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121 containing the GUS and nptII genes. GUS histochemical assay and PCR analysis were conducted on rooted shoots grown in media without hormones but supplemented with antibiotics. Transformed shoots tested positive through GUS histochemical assay and integration of nptII gene was confirmed by PCR analysis DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14193 Plant Tissue Cult. & Biotech. 22(2): 93-105, 2012 (December)

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Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

Onamu

Legaria

Sahagún

et al. 2013

Plant Tissue Cult. & Biotech.

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“…Using the hormone combinations of 2,4-D and zeatin riboside showed a higher regeneration rate compared to other protocols but an intermediate callus phase increased the time required for regeneration and also increases the likelihood of obtaining somaclonal variants. Direct shoot regeneration from the explants without introducing a callus phase gives a much higher regeneration efficiency [16,17]. The addition of t-zeatin or zeatin riboside to the medium was found to be more effective for regeneration of shoots and also produced less calli (Figure 1(b)) as compared to those without zeatin [18].…”

Section: Influence Of Explants and Hormonesmentioning

confidence: 99%

Rapid regeneration of stable transformants in cultures of potato by improving factors influencing Agrobacterium-mediated transformation

Chakravarty¹,

Wang‐Pruski²

2010

ABB

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An efficient and rapid Agrobacterium tumefaciens-mediated transformation protocol was developed to generate activation-tagged mutant lines with the aim of large-scale functional analysis of the potato genome. The explants were inoculated with an Agrobacterium strain harboring the binary plasmid pSKI074 containing four CaMV 35S enhancers in the T-DNA region which activates the downstream genes in the host plant after its integration. Various parameters investigated to increase transformation efficiency were the type and age of explant, cultivar, hormone combinations, preculture of explants, period of co-cultivation with bacteria and concentration of bacterial cultures used for transformation. Stem explants from 5 week old plantlets of cv. Bintje which had undergone phytohormone pretreatment for 4 days, inoculation with diluted bacterial concentration of OD600 = 0.2 containing acetosyringone followed by 2 days of co-cultivation and selection in media with IAA and trans-zeatin all helped in greatly improving the transformation efficiency. The total time required from infection to rooted shoots was 6-7 weeks. Initial evidence for stable integration and expression of the transgenes by PCR analysis showed that over 93% of the regenerated lines were transgenic and this was confirmed by Southern hybridization

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“…Plant regeneration and transformation in potato has been reported using a wide range of explants including leaves, stems, tubers, and microtubers (Mitten et al, 1990;Visser, 1991;Snyder and Belknap, 1993;Dale and Hampton, 1995;Chang et al, 2002); using a variety of germplasm, including diploids, tetraploids, and Solanum species (Visser et al, 1989;Kumar et al, 1995;Park et al, 1995;Beaujean et al, 1998;Trujillo et al, 2001); and using several different methods (De Block, 1988;Wenzler et al, 1989;Sidorov et al, 1999;Romano et al, 2001). The number of plants produced is usually low for most varieties and dependent on the genotype used (Wenzler et al, 1989;Yee et al, 2001).…”

Section: Introductionmentioning

confidence: 97%

Transformation and Plant Regeneration from Leaf Explants of Solanum tuberosum L. cv. ‘Shepody’

Gustafson

Mallubhotla

MacDonnell³

et al. 2006

Plant Cell Tiss Organ Cult

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As part of a large-scale genomics project focused on understanding and improving the Shepody potato, we have increased the regeneration and transformation rates for this cultivar. Using combinations of auxins and trans-zeatin, leaf and stem explants were evaluated for callus induction and shoot formation. Several plant growth regulator combinations resulted in higher plant regeneration rates over a previous method. Using the best combination of auxin and cytokinin in combination with Agrobacterium-mediated transformation, we regenerated independent putative transformants from 59.5% of the total explants plated. We ran PCR on a sample of the plants to confirm transformation and 47.1% were nptII positive; giving a confirmed transformation rate of 28.0%.

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One-step transformation of two Andean potato cultivars (Solanum tuberosum L. subsp. andigena)

Cited by 28 publications

References 9 publications

In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

Rapid regeneration of stable transformants in cultures of potato by improving factors influencing Agrobacterium-mediated transformation

Transformation and Plant Regeneration from Leaf Explants of Solanum tuberosum L. cv. ‘Shepody’

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