One-step Synthesis of Silver Nanoparticles Using Saudi Arabian Desert Seasonal Plant Sisymbrium irio and Antibacterial Activity Against Multidrug-Resistant Bacterial Strains

Abstract

Globally, antimicrobial resistance has grown at an alarming rate. To combat the multidrug-resistant (MDR) superbugs, silver nanoparticles (Ag NPs) were synthesized using an aqueous leaf extract of seasonal desert plant Sisymbrium irio obtained from the central region of Saudi Arabia by a simple one-step procedure. The physical and chemical properties of the Ag NPs were investigated through ultraviolet visisble analysis (UV-vis), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM) analysis. The UV-vis spectrum showed an absorption band at 426 nm. The XRD results showed a highly crystalline face-centered cubic structure. The surface morphology analyzed using SEM and TEM analyses showed the particle size to be in the range 24 nm to 50 nm. Various concentrations of Ag NPs were tested against MDR Pseudomonas aeruginosa and Acinetobacter baumanii that cause ventilator-associated pneumonia (VAP). American Type Culture Collection (ATCC) Escherichia coli-25922 was used as the reference control strain. The Ag NPs effectively inhibited tested pathogens, even at the lowest concentration (6.25 µg) used. The bacterial inhibitory zone ranged from 11–21 mm. In conclusion, the newly synthesized Ag NPs could be a potential alternative candidate in biomedical applications in controlling the spread of MDR pathogens.

Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii

“…01-087-0717. Peaks indicated by asterisks in Figure 3B probably correspond to the crystallization of the bio-organic phase occurring on the AgNPs surface, as also reported in [53][54][55][56][57]. Alternatively, these peaks may be due to AgNO3, which has not been reduced by Pseudomonas sp.…”

Hydrophilic Quaternary Trimethyl Chitosan Stabilized Silver Nanoparticles: Synthesis, Characterization and Antimicrobial Activity.

“…The images revealed uniformly distributed spherical shapes. This morphological behavior has been reported to be common to silver nanoparticles in previous studies [6,17,20,34].…”

Gold Nanoparticles Synthesized Using Extracts of Cyclopia intermedia, Commonly Known as Honeybush, Amplify the Cytotoxic Effects of Doxorubicin

“…This band is also assigned to the C-N stretching of aliphatic amines [ 43 ] mentioned earlier at 3747 cm −1 peak, thereby confirming the involvement of aliphatic amines in the formation of the HB-AuNPs. The weak bands at 2103 and 2140 cm −1 corresponded to C=N stretching, an indication that amides may also be involved in the synthesis of the HB-AuNPs [ 44 ].…”