One-step rapid quantification of SARS-CoV-2 virus particles via low-cost nanoplasmonic sensors in generic microplate reader and point-of-care device

Huang, Liping; Ding, Longfei; Zhou, Jun; Chen, Shouhui; Chen, Fang; Zhao, Chen; Xu, Jianqing; Hu, Wenjun; Ji, Jiansong; Xu, Hao; Liu, Gang L.

doi:10.1016/j.bios.2020.112685

Cited by 193 publications

(170 citation statements)

References 39 publications

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“…Although they may present future use as point-of-care diagnostics, further work still needs to be done in order to miniaturize and lower the cost of these biosensors. Currently, optical, electrochemical, and molecularly imprinted biosensors are being designed for the detection of COVID-19 [143][144][145][146][147][148][149], as seen in Table 1:…”

Section: Severe Acute Respiratory Syndrome Coronavirus 2 Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

2021

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The last few decades have been plagued by viral outbreaks that present some of the biggest challenges to public safety. The current coronavirus (COVID-19) disease pandemic has exponentiated these concerns. Increased research on diagnostic tools is currently being implemented in order to assist with rapid identification of the virus, as mass diagnosis and containment is the best way to prevent the outbreak of the virus. Accordingly, there is a growing urgency to establish a point-of-care device for the rapid detection of coronavirus to prevent subsequent spread. This device needs to be sensitive, selective, and exhibit rapid diagnostic capabilities. Electrochemical biosensors have demonstrated these traits and, hence, serve as promising candidates for the detection of viruses. This review summarizes the designs and features of electrochemical biosensors developed for some past and current pandemic or epidemic viruses, including influenza, HIV, Ebola, and Zika. Alongside the design, this review also discusses the detection principles, fabrication techniques, and applications of the biosensors. Finally, research and perspective of biosensors as potential detection tools for the rapid identification of SARS-CoV-2 is discussed.

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Section: Severe Acute Respiratory Syndrome Coronavirus 2 Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

2021

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“…The as proposed SERS-LFIA biosensor showed a detection limit of 800 times higher than Au NPs based LFIA. The direct detection of S protein has been achieved by using nanoplasmonic resonance sensors with a detection limit of 370 vp/mL in 15 min [ 80 ]. The assay was very economical showing good performance under clinical environment.…”

Section: Biosensing Techniques For Sars-cov-2 Detementioning

confidence: 99%

“… Biosensor Pathogen Detection target Limit of detection Linear range Ref. EC SARS-CoV-2 S or N protein 19 ng/mL and 8 ng/mL - [ 103 ] EC SARS-CoV-2 Antibodies - - [ 101 ] EC SARS-CoV-2 N-gene 6.9 copies/μL 585.4 to 5.854 × 10 7 copies/μL [ 99 ] Cell-based SARS-CoV-2 Antigen 1 fgmL 1- 10 fg and 1 μg mL 1- [ 105 ] Optofluidic SARS-CoV-2 Antibody 0.5 pM 1- [ 83 ] Nanoplasmonic SARS-CoV-2 Virus particles 370 vp/mL 0 to 10 7 vp/mL [ 80 ] SPR SARS-CoV-2 Antibody 1.02 pM 2-1000 pM [ 82 ] LSPR SARS-CoV-2 RNA 1 pM 1 nM to 1 μM [ 106 ] Lateral flow optical/chemiluminescence SARS-CoV-2 Serum IgA - - [ 78 ] SERS-LFIA SARS-CoV-2 Antibody 1.28×10 7 -fold dilution - [ 79 ] Lateral flow SARS-CoV-2 RNA 12 copies...…”

Section: Biosensing Techniques For Sars-cov-2 Detementioning

confidence: 99%

Trends in biosensing platforms for SARS-CoV-2 detection: A critical appraisal against standard detection tools

Aziz

Asif

Ashraf

et al. 2021

Current Opinion in Colloid & Interface Science

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“…For dissolving the immunoreagents, both bovine serum albumin (BSA) and a phosphate buffer solution (PBS) were used. To enhance the binding of biomarkers to the sensor surface, functionalize [47], b naked-eye detection of SARS-CoV-2 by ASO capped gold nanoparticles [49], c PRAM-based AC + DC immunoassay [54] AuNPs were dispersed on the biosensor's surface. The ultratight field confinement of toroidal metasensor makes it possible to achieve high sensitivity like the LSPR biosensors.…”

Section: Plasmonic Biosensorsmentioning

confidence: 99%

Nanomaterials to tackle the COVID-19 pandemic

Pishva

Yüce

2021

emergent mater.

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The rapid worldwide spread of the COVID-19 pandemic, caused by the severe acute respiratory SARS-CoV-2, has created an urgent need for its diagnosis and treatment. As a result, many researchers have sought to find the most efficient and appropriate methods to detect and treat the SARS-CoV-2 virus over the past few months. Real-time reverse-transcriptase polymerase chain reaction (RT-PCR) testing is currently used as one of the most reliable methods to detect the new virus; however, this method is time-consuming, labor-intensive, and requires trained laboratory workers. Moreover, despite its high sensitivity and specificity, false negatives are reported, especially in non-nasopharyngeal swab samples that yield lower viral loads. Therefore, designing and employing faster and more reliable methods seems necessary. In recent years, many attempts have been made to fabricate various nanomaterial-based biosensors to detect viruses and bacteria in clinical samples. The use of nanomaterials plays a significant role in improving the performance of biosensors. Plasmonic biosensors, field-effect transistor (FET)-based biosensors, electrochemical biosensors, and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods are only some of the effective ways to detect viruses. However, to use these biosensors to detect the SARS-CoV-2 virus, modifications must be performed to increase sensitivity and speed of testing due to the rapidly spreading nature of SARS-CoV-2, which requires an early point of care detection and treatment for pandemic control. Several studies have been carried out to show the nanomaterial-based biosensors' performance and success in detecting the novel virus. The limit of detection, accuracy, selectivity, and detection speed are some vital features that should be considered during the design of the SARS-CoV-2 biosensors. This review summarizes various nanomaterials-based sensor platforms to detect the SARS-CoV-2, and their design, advantages, and limitations.

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One-step rapid quantification of SARS-CoV-2 virus particles via low-cost nanoplasmonic sensors in generic microplate reader and point-of-care device

Cited by 193 publications

References 39 publications

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

Electrochemical Biosensors for the Detection of SARS-CoV-2 and Other Viruses

Trends in biosensing platforms for SARS-CoV-2 detection: A critical appraisal against standard detection tools

Nanomaterials to tackle the COVID-19 pandemic

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