One‐Step Rapid Fabrication of Cell‐Only Living Fibers

Sousa, Ana Rita; Martins-Cruz, Cláudia; Oliveira, Mariana B.

doi:10.1002/adma.201906305

Cited by 23 publications

(23 citation statements)

References 68 publications

(109 reference statements)

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“…Preparation of SH: Polystyrene petri dishes were treated according to previous reports. [39] Briefly, a layer of an air-cured coating agent (WX2100, Cytonix) was created onto petri dishes surfaces. After drying for 48 h, the excess of the sprayed solution was removed and submerged in a solution of 70% ethanol for at least 3 days.…”

Section: Methodsmentioning

confidence: 99%

Design of Protein‐Based Liquefied Cell‐Laden Capsules with Bioinspired Adhesion for Tissue Engineering

Gomes

Costa

Oliveira

et al. 2021

Adv Healthcare Materials

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Platforms with liquid cores are extensively explored as cell delivery vehicles for cell-based therapies and tissue engineering. However, the recurrence of synthetic materials can impair its translation into the clinic. Inspired by the adhesive proteins secreted by mussels, liquefied capsule is developed using gelatin modified with hydroxypyridinones (Gel-HOPO), a catechol analogue with oxidant-resistant properties. The protein-based liquefied macrocapsule permitted the compartmentalization of living cells by an approachable and non-time-consuming methodology resorting to i) superhydrophobic surfaces as a processing platform of hydrogel beads, ii) gelation of gelatin at temperatures < 25°C, iii) iron coordination of the hydroxypyridinone (HOPO) moieties at physiological pH, and iv) core liquefaction at 37°C. With the design of a proteolytically degradable shell, the possibility of encapsulating human adipose-derived mesenchymal stem cells (hASC) with and without the presence of polycaprolactone microparticles ( PCL) is evaluated. Showing prevalence toward adhesion to the inner shell wall, hASC formed a monolayer evidencing the biocompatibility and adequate mechanical properties of these platforms for proliferation, diminishing the need for PCL as a supporting substrate. This new protein-based liquefied platform can provide biofactories devices of both fundamental and practical importance for tissue engineering and regenerative medicine or in other biotechnology fields.

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Section: Methodsmentioning

confidence: 99%

Design of Protein‐Based Liquefied Cell‐Laden Capsules with Bioinspired Adhesion for Tissue Engineering

Gomes

Costa

Oliveira

et al. 2021

Adv Healthcare Materials

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“…Newer extrusion techniques with lower speed can produce high-density constructs with radial and axial patterning [ 34 , 35 ] but have difficulty in creating branching networks and require specialized equipment. Alternatively, the hanging drop method has been modified with patterned substrates in rectangularly designed hydrophilic regions to confine cells in a semi-cylindrical fashion to assemble ECM-free fibers [ 36 ]. This interesting approach is an advancement over the traditional hanging drop method but is still limited in its ability to form multicellular patterns radially or axially.…”

Section: Resultsmentioning

confidence: 99%

Tissue-in-a-Tube: three-dimensional in vitro tissue constructs with integrated multimodal environmental stimulation

Shahin‐Shamsabadi

Selvaganapathy

2020

Materials Today Bio

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Three-dimensional (3D) in vitro tissue models are superior to two-dimensional (2D) cell cultures in replicating natural physiological/pathological conditions by recreating the cellular and cell-matrix interactions more faithfully. Nevertheless, current 3D models lack either the rich multicellular environment or fail to provide appropriate biophysical stimuli both of which are required to properly recapitulate the dynamic in vivo microenvironment of tissues and organs. Here, we describe the rapid construction of multicellular, tubular tissue constructs termed Tissue-in-a-Tube using self-assembly process in tubular molds with the ability to incorporate a variety of biophysical stimuli such as electrical field, mechanical deformation, and shear force of the fluid flow. Unlike other approaches, this method is simple, requires only oxygen permeable silicone tubing that molds the tissue construct and thin stainless-steel pins inserted in it to anchor the construct and could be used to provide electrical and mechanical stimuli, simultaneously. The annular region between the tissue construct and the tubing is used for perfusion. Highly stable, macroscale, and robust constructs anchored to the pins form as a result of self-assembly of the extracellular matrix (ECM) and cells in the bioink that is filled into the tubing. We demonstrate patterning of grafts containing cell types in the constructs in axial and radial modes with clear interface and continuity between the layers. Different environmental factors affecting cell behavior such as compactness of the structure and size of the constructs can be controlled through parameters such as initial cell density, ECM content, tubing size, as well as the distance between anchor pins. Using connectors, network of tubing can be assembled to create complex macrostructured tissues (centimeters length) such as fibers that are bifurcated or columns with different axial thicknesses which can then be used as building blocks for biomimetic constructs or tissue regeneration. The method is versatile and compatible with various cell types including endothelial, epithelial, skeletal muscle cells, osteoblast cells, and neuronal cells. As an example, long mature skeletal muscle and neuronal fibers as well as bone constructs were fabricated with cellular alignment dictated by the applied electrical field. The versatility, speed, and low cost of this method is suited for widespread application in tissue engineering and regenerative medicine.

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“…In an intriguing study, the Mano group recently showed that mammalian cells are also able to form desired three-dimensional structures such as fibers without the need for genetic or chemical modification but simply by providing a sophisticated cell culture environment [ 196 ]. In their work, they placed a hydrophilic, wettable line surrounded by superhydrophobic areas in a culture dish.…”

Section: Elmsmentioning

confidence: 99%

“…Driven by gravity, the cells accumulated at the bottom and started building intercellular connections. The length and thickness of these fibers could be tuned by the initial cell concentration and it was possible to grow fibers made from several different cell types together [ 196 ]. The Joshi group recently used a similar approach to synthesize stiff living materials [ 198 ].…”

Section: Elmsmentioning

confidence: 99%

Synthetic biology as driver for the biologization of materials sciences

Burgos-Morales

Gueye

Lacombe

et al. 2021

Materials Today Bio

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Materials in nature have fascinating properties that serve as a continuous source of inspiration for materials scientists. Accordingly, bio-mimetic and bio-inspired approaches have yielded remarkable structural and functional materials for a plethora of applications. Despite these advances, many properties of natural materials remain challenging or yet impossible to incorporate into synthetic materials. Natural materials are produced by living cells, which sense and process environmental cues and conditions by means of signaling and genetic programs, thereby controlling the biosynthesis, remodeling, functionalization, or degradation of the natural material. In this context, synthetic biology offers unique opportunities in materials sciences by providing direct access to the rational engineering of how a cell senses and processes environmental information and translates them into the properties and functions of materials. Here, we identify and review two main directions by which synthetic biology can be harnessed to provide new impulses for the biologization of the materials sciences: first, the engineering of cells to produce precursors for the subsequent synthesis of materials. This includes materials that are otherwise produced from petrochemical resources, but also materials where the bio-produced substances contribute unique properties and functions not existing in traditional materials. Second, engineered living materials that are formed or assembled by cells or in which cells contribute specific functions while remaining an integral part of the living composite material. We finally provide a perspective of future scientific directions of this promising area of research and discuss science policy that would be required to support research and development in this field.

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One‐Step Rapid Fabrication of Cell‐Only Living Fibers

Cited by 23 publications

References 68 publications

Design of Protein‐Based Liquefied Cell‐Laden Capsules with Bioinspired Adhesion for Tissue Engineering

Design of Protein‐Based Liquefied Cell‐Laden Capsules with Bioinspired Adhesion for Tissue Engineering

Tissue-in-a-Tube: three-dimensional in vitro tissue constructs with integrated multimodal environmental stimulation

Synthetic biology as driver for the biologization of materials sciences

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