One-step purification and concentration of DNA in porous membranes for point-of-care applications

Byrnes, Samantha A.; Bishop, Joshua D.; Lafleur, Lisa; Buser, Joshua R.; Lutz, Barry; Yager, Paul

doi:10.1039/c5lc00317b

Cited by 81 publications

(72 citation statements)

References 75 publications

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“…[16][17][18] Recent advances in µPADs position these technologies for broad diagnostic application. [19][20][21][22] Specifically, the integration of nanomaterials into paper potentially offers enhanced detection capabilities. 23 Nanoparticles are the most commonly employed nanomaterial in lateral flow assays and µPADs, and are mainly used as labels for colorimetric detection of DNA and other analytes.…”

Section: Introductionmentioning

confidence: 99%

Direct DNA Analysis with Paper-Based Ion Concentration Polarization

et al. 2015

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DNA analysis is essential for diagnosis and monitoring of many diseases. Conventional DNA testing is generally limited to the laboratory. Increasing access to relevant technologies can improve patient care and outcomes in both developed and developing regions. Here, we demonstrate direct DNA analysis in paper-based devices, uniquely enabled by ion concentration polarization at the interface of patterned nanoporous membranes in paper (paper-based ICP). Hepatitis B virus DNA targets in human serum are simultaneously preconcentrated, separated, and detected in a single 10 min operation. A limit of detection of 150 copies/mL is achieved without prior viral load amplification, sufficient for early diagnosis of hepatitis B. We clinically assess the DNA integrity of sperm cells in raw human semen samples. The percent DNA fragmentation results from the paper-based ICP devices strongly correlate (R(2) = 0.98) with the sperm chromatin structure assay. In all cases, agreement was 100% with respect to the clinical decision. Paper-based ICP can provide inexpensive and accessible advanced molecular diagnostics.

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Section: Introductionmentioning

confidence: 99%

Direct DNA Analysis with Paper-Based Ion Concentration Polarization

et al. 2015

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“…Examples of these products are FTAcard, [16][17][18][19][20][21][22] chitosancoated paper, [23,24] glass-fiber paper, [25][26][27][28] and polyethersulfone filter paper. Examples of these products are FTAcard, [16][17][18][19][20][21][22] chitosancoated paper, [23,24] glass-fiber paper, [25][26][27][28] and polyethersulfone filter paper.…”

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confidence: 99%

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

et al. 2018

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The recent outbreaks of Zika virus (ZIKV) infection represent ap ublic health challenge.R apid, cost-effective,a nd reliable diagnostic tools for ZIKV detection at the point of care (POC) are highly desirable,e specially for resource-limited nations.T oaddress the need, we have developed an integrated device to achieve sample-to-answer ZIKV detection. The device features innovative ball-based valves enabling the storage and sequential delivery of reagents for virus lysis and apaper-based unit for RNAenrichmentand purification. The paper unit is placed in ac ommercially available coffee mug that provides ac onstant temperature for reverse transcription loop-mediated isothermal amplification (RT-LAMP), followed by colorimetric detection by naked eye or ac ellphone camera. Using the device,w ed emonstrated the reproducible detection of ZIKV in human urine and saliva samples.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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“…Unfortunately, advances in on-chip SPrep have been overlooked in comparison to downstream processes like analysis and sensing (Brehm-Stecher, et al, 2009;Mariella Jr, 2008;Kim, et al, 2009). The dependence on traditional offchip sample pre-treatment involving expensive equipment and trained personnel has prevented the translation of these advances to POC (Byrnes, et al, 2015).…”

Section: Microfluidic Sample Preparationmentioning

confidence: 99%

Advances in Microfluidics and Lab-on-a-Chip Technologies

Jayamohan

Romanov

et al. 2017

Molecular Diagnostics

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Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense. The emergence of microfluidics and nanotechnologies has enabled both new capabilities and instrument sizes practical for point-of-care. It has also introduced new functionality, enhanced sensitivity, and reduced the time and cost involved in conventional molecular diagnostic techniques. This chapter reviews the application of microfluidics for molecular diagnostics methods such as nucleic acid amplification, next-generation sequencing, high resolution melting analysis, cytogenetics, protein detection and analysis, and cell sorting. We also review microfluidic sample preparation platforms applied to molecular diagnostics and targeted to sample-in, answer-out capabilities

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One-step purification and concentration of DNA in porous membranes for point-of-care applications

Cited by 81 publications

References 75 publications

Direct DNA Analysis with Paper-Based Ion Concentration Polarization

Direct DNA Analysis with Paper-Based Ion Concentration Polarization

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

Advances in Microfluidics and Lab-on-a-Chip Technologies

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