One-Step Preparation of Chitosan-Coated Cationic Liposomes by an Improved Supercritical Reverse-Phase Evaporation Method

Otake, Katsuto; Shimomura, Takeshi; Goto, Toshihiro; Imura, Tomohiro; Furuya, Takeshi; Yoda, Satoshi; Takebayashi, Yoshihiro; Sakai, Hideki; Abe, Masahiko

doi:10.1021/la051662a

Cited by 61 publications

(29 citation statements)

References 36 publications

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“…Simplified representation of the apparatus used in the scRPE method (33) carbonic acids incorporated into the bilayer membrane. The addition of chitosan to the liposomal formulation in the ISCRPE method did not affect its stability (30 days) and a trapping efficiency of 17% was achieved (42).…”

Section: Supercritical Reverse Phase Evaporation Processmentioning

confidence: 97%

Conventional and Dense Gas Techniques for the Production of Liposomes: A Review

2008

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Abstract. The aim of this review paper is to compare the potential of various techniques developed for production of homogenous, stable liposomes. Traditional techniques, such as Bangham, detergent depletion, ether/ethanol injection, reverse-phase evaporation and emulsion methods, were compared with the recent advanced techniques developed for liposome formation. The major hurdles for scaling up the traditional methods are the consumption of large quantities of volatile organic solvent, the stability and homogeneity of the liposomal product, as well as the lengthy multiple steps involved. The new methods have been designed to alleviate the current issues for liposome formulation. Dense gas liposome techniques are still in their infancy, however they have remarkable advantages in reducing the use of organic solvents, providing fast, single-stage production and producing stable, uniform liposomes. Techniques such as the membrane contactor and heating methods are also promising as they eliminate the use of organic solvent, however high temperature is still required for processing.

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Section: Supercritical Reverse Phase Evaporation Processmentioning

confidence: 97%

Conventional and Dense Gas Techniques for the Production of Liposomes: A Review

2008

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“…[13] On the other hand, the solubility of chitosan in scCO 2 possibly may be induced by the presence of water. [14] But our purpose at this stage was not to apply any liquid cosolvents, including water. Thus we paid special attention to remove traces of water from the reactor initially by means of preliminary purging of the sealed system with CO 2 flow before the real exposure.…”

Section: Resultsmentioning

confidence: 99%

Chitosan Molecules Deposited from Supercritical Carbon Dioxide on a Substrate: Visualization and Conformational Analysis

Gallyamov

Chaschin

Gamzazade

et al. 2008

Macro Chemistry & Physics

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We deposited single individual macromolecules of chitosan directly from their solutions in supercritical carbon dioxide onto a substrate surface and applied SFM method to analyze the macromolecular conformation. By means of image processing of many different SFM‐contours of the visualized individual macromolecules, we determined the scaling exponent ν, which describes the correlation between mean square end‐to‐end distances and contour lengths of the adsorbed macromolecules. It was revealed, that the macromolecules, deposited from scCO2 and visualized by SFM, tended to adopt rather extended conformation. The scaling exponent ν was found to be in range of 0.78–0.8, which was slightly above the 3/4 value, corresponding to 2D self‐avoiding walk statistics. Good adhesion of chitosan coatings directly deposited from scCO2 is expected.

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“…Using the scRPE method, nano-sized liposomes were successfully formed in a one-step procedure [97,98]. Figure 10) to produce liposomes [85]. The process avoids the use of organic solvents, reduces the risk of liposome disruption and increases encapsulation efficiency compared to scRPE [19,85].…”

Section: Supercritical Reverse Phase Evaporation (Scrpe)mentioning

confidence: 99%

Formation of Nanocarrier Systems by Dense Gas Processing

2014

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Nanocarrier systems, such as liposomes, polymersomes, and micelles, find applications in the delivery of a wide range of compounds, including targeted delivery of pharmaceuticals. Nanocarrier systems have the ability to increase the bioavailability, reduce toxicity, and avoid undesirable interactions of active pharmaceutical ingredients. In this work, a novel dense gas technique known as depressurization of an expanded solution into aqueous media (DESAM) was used to produce different types of nanocarrier systems. The effects of using different types of dense gases and different operating temperatures were investigated. Encapsulation of hydrophilic compounds in the vesicles (liposomes and polymersomes) was also studied. The highest encapsulation efficiencies in liposomes and polymersomes achieved were 10.2 and 9.7%, respectively. The DESAM process was also able to reduce the residual solvent content in the product to 2.2% (v/v), which is significantly lower than the solvent residual levels reported for conventional processing.

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One-Step Preparation of Chitosan-Coated Cationic Liposomes by an Improved Supercritical Reverse-Phase Evaporation Method

Cited by 61 publications

References 36 publications

Conventional and Dense Gas Techniques for the Production of Liposomes: A Review

Conventional and Dense Gas Techniques for the Production of Liposomes: A Review

Chitosan Molecules Deposited from Supercritical Carbon Dioxide on a Substrate: Visualization and Conformational Analysis

Formation of Nanocarrier Systems by Dense Gas Processing

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