One Step Nucleic Acid Amplification (OSNA) positive micrometastases and additional histopathological NSLN metastases: Results from a single institution over 53 months

Babar, Mahwash; Madani, Rana; Jackson, Peter A.; Layer, G. T.; Kissin, Mark; Irvine, Tracey

doi:10.1016/j.surge.2014.06.001

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…Patients with macrometastasis in their SLNBs (41.5%–50.3%) had a greater incidence of axillary node positivity than those with a micrometastasis (17.9%–26.8%) 26–28. Using the OSNA system, Babar et al 29 reported that 26.7% of OSNA-positive SLNB had positive ACLNs. Thirty-eight per cent of patients with macrometastasis and 15% with micrometastasis had positive ACLNs.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Metasin assay for intraoperative assessment of sentinel lymph node metastases in breast cancer

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Metasin assay for intraoperative assessment of sentinel lymph node metastases in breast cancer

et al. 2016

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“…By contrast, the ICS assesses the entire lymph node, that is, it is a comprehensive examination of all tissue in the lymph node, and can thus detect small lesions ( Celebioglu et al, 2006 ). Many researchers have applied one-step nucleic acid amplification (OSNA) technology to detect levels of CK-19 mRNA in sentinel lymph nodes of patients with breast cancer to determine whether there is metastasis ( Klingler et al, 2013 ; Babar et al, 2016 ; Shimazu et al, 2019 ). However, owing to the amplification of pseudogenes of genomic DNA using this technique, false-positive results are not uncommon.…”

Section: Discussionmentioning

confidence: 99%

Development of a Colloidal Gold Immunochromatographic Strip for Rapid Detection of Cyfra 21-1 in Lymph Node Metastasis of Thyroid Cancer

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Thyroid cancer is the most common endocrine tumor, and the rate of early lymph node metastasis may be as high as 60%. Currently, detection of lymph node metastasis of thyroid cancer during surgery is limited and time-consuming. Elevated levels of Cyfra 21-1, the proteolytic portion of cytokeratin, are associated with the metastasis and progression of thyroid cancer and are an effective biomarker for the prognosis and diagnosis of thyroid cancer. In this study, an immunochromatographic strip test based on colloidal gold nanoparticles was developed to semi-quantitatively detect the levels of Cyfra 21-1 in lymph nodes within 15 min. The standard (calibration) curve equation was Y = 0.003708 × X + 0.1101, and the detection limit was 0.55–1.14 ng mL−1. The strip did not detect other protein markers of epithelial cells at a concentration of 500 ng mL−1, including cytokeratin 8, cytokeratin 18, epithelial membrane antigen, and epidermal surface antigen. The ability of the strip to differentiate positive from negative metastasis in 40 lymph node specimens was 100% concordant with that of immunohistochemical staining for Cyfra 21-1. In an assessment of 20 lymph node specimens that had been determined by postoperative histopathology to be positive for lymph node metastasis and 20 specimens that were negative, the sensitivity and specificity of the strip were 100% and 95%, respectively. The sensitivity of the strip remained stable when stored at room temperature for 6 months. Together, these results indicated that although further testing using a larger sample size will be required, this immunochromatographic strip test may be useful for rapid intraoperative detection of thyroid cancer metastasis to lymph nodes.

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“…Step Nucleic Acid Amplification (OSNA) is a highly sensitive, automated and rapid assay that analyses lymph nodes for identifying metastases by detection and amplification of the cytokeratin 19 (CK19) mRNA, an epithelial marker in BC cells (37). Several studies demonstrated that the NSLN macro-metastatic rate increased in proportion to CK 19 mRNA copy numbers (38) (38), who observed that using OSNA technique to evaluate SLN, NSLN metastases can be predicted intraoperatively, supporting in decision for ALND.…”

Section: Intraoperative Assessment Of Sentinel Nodesmentioning

confidence: 99%

Axillary lymph node surgical treatment

et al. 2018

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Nowadays, the overall attention is focused on de-escalating treatments for breast cancer (BC) including surgery, radiotherapy and chemotherapy. The introduction of sentinel lymph node biopsy (SLNB) has led to less invasive surgical approaches for accurately staging the axilla, with axillary lymph node dissection (ALND) progressively confined to a limited group of patients. One of the goal of surgery in de-escalating approaches is to reduce surgical morbidity by restricting or avoiding axillary surgery with no effect on survival. In this context the importance of imaging study for preoperative identification of axillary metastasis, in order to reduce axillary surgery, is gradually improving while the role of intraoperative assessment of sentinel nodes is progressively becoming limited to restricted groups of patients. According to the results of the ACOSOG Z0011 and following the most important guidelines, ALND can be safely omitted in selected patients treated with breast conserving surgery (BCS) with one or two positive SLNB while the adoption of SLNB positive alone in patients undergoing mastectomy is not yet defined. The increased employment of neoadjuvant chemotherapy (NAC) and the use of SLNB in patients after NAC plays an important role in de-escalation of axillary surgery in this group of patients. However current studies on this topic are still controversial, mainly about clinically positive lymph nodes (cN+) pre NAC patients or how to manage positive SLNB in post NAC patients. Some authors have collected predictive factors of positive non sentinel lymph nodes (NSLNs) in nomograms, considered an useful tool to avoid unnecessary further surgery. Elderly women represent specific group of patients where the axillary approach needs to be properly resized. The management of axilla in BC is in continuous evolution and ongoing studies could make even SLNB useless in the next future.

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One Step Nucleic Acid Amplification (OSNA) positive micrometastases and additional histopathological NSLN metastases: Results from a single institution over 53 months

Cited by 5 publications

References 41 publications

Evaluation of the Metasin assay for intraoperative assessment of sentinel lymph node metastases in breast cancer

Evaluation of the Metasin assay for intraoperative assessment of sentinel lymph node metastases in breast cancer

Development of a Colloidal Gold Immunochromatographic Strip for Rapid Detection of Cyfra 21-1 in Lymph Node Metastasis of Thyroid Cancer

Axillary lymph node surgical treatment

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