One-step multiplex PCR method for the determination of pecan and Brazil nut allergens in food products

Hubálková, Zora; Rencová, E

doi:10.1002/jsfa.4479

Cited by 17 publications

(2 citation statements)

References 32 publications

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“…The sensitivity of our multiplex PCR assay was comparable with allergen reported previously. Hubalkova and Rencova (2011) reported detection limits of 100 pg for pecan and Brazil nut allergy. Zhang et al (2018) reported detection limits of 10-100 pg for peanut, soybean and sesame allergy.…”

Section: Specificity and Sensitivity Test Of The Multiplex Pcr Assaymentioning

confidence: 99%

Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR

Suh

Park

Kim

et al. 2019

Food Sci Biotechnol

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Fruit allergies have become more common in recent years, and are now a serious health problem. In this study, a multiplex PCR assay was used to detect potential fruit allergens causing food allergy labeling in Korea. For the detection of these allergens, specific primer pairs were designed to amplify the allergen-coding genes Cyclophilin (tomato), Mdtl 1 (apple), Pru p 2.01A (peach) and Pectin methylesterase inhibitor (kiwi), and primer pair targeting the 18S ribosomal RNA gene was additionally used as an endogenous control. Primer specificity was assessed with 23 plant species. A mixture of DNA from the four fruits was serially diluted and used to determine the sensitivity of the multiplex PCR assay, which was approximately 0.08 ng. Eleven commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. This assay is expected to be a specific and efficient method for detecting fruit allergens in foods.

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Section: Specificity and Sensitivity Test Of The Multiplex Pcr Assaymentioning

confidence: 99%

Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR

Suh

Park

Kim

et al. 2019

Food Sci Biotechnol

View full text Add to dashboard Cite

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“…These conditions are dangerous if the contaminant taxon is toxic or allergenic for humans. A typical example is that of nuts and almonds which cause allergies in many people [91]. Several commercial foodstuffs (e.g., bakery, pastry, and snacks) showed contamination by these plants (see, e.g., [76,92]).…”

Section: Honeymentioning

confidence: 99%

DNA Barcoding for Minor Crops and Food Traceability

Galimberti

Labra

Sandionigi

et al. 2014

Advances in Agriculture

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This outlook paper addresses the problem of the traceability of minor crops. These kinds of cultivations consist in a large number of plants locally distributed with a modest production in terms of cultivated acreage and quantity of final product. Because of globalization, the diffusion of minor crops is increasing due to their benefit for human health or their use as food supplements. Such a phenomenon implies a major risk for species substitution or uncontrolled admixture of manufactured plant products with severe consequences for the health of consumers. The need for a reliable identification system is therefore essential to evaluate the quality and provenance of minor agricultural products. DNA-based techniques can help in achieving this mission. In particular, the DNA barcoding approach has gained a role of primary importance thanks to its universality and versatility. Here, we present the advantages in the use of DNA barcoding for the characterization and traceability of minor crops based on our previous or ongoing studies at the ZooPlantLab (Milan, Italy). We also discuss how DNA barcoding may potentially be transferred from the laboratory to the food supply chain, from field to table.

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Recent Advances in the Detection of Allergens in Foods

Cruz

López-Calleja

Martı́n

et al. 2017

Methods in Molecular Biology

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Food allergy is a public health issue that has significantly increased worldwide in the past decade affecting consumers' quality of life and making increasing demands on health service resources. Despite recent advances in many areas of diagnosis and treatment, our general knowledge of the basic mechanisms of the disease remained limited, i.e., not at pace with the exponential number of new cases and the explosion of the new technologies. For sensitized individuals, the only effective way to prevent allergic reactions is the strict avoidance of the offending food. For this reason, a number of regulatory bodies in several countries have recognized the importance of providing information about the presence of food allergens by enacting laws, regulations, or standards for food labeling of "priority allergens." This has resulted in the need for the development of analytical methods for protection of food-allergic consumers that should be among others highly specific, sensitive, and not influenced by the presence of the food matrix components. Several analytical approaches target either the allergen itself or a corresponding allergen marker such as peptide fragment or gene segment and have been used in the detection and quantification of allergens in food products. In this short review, some of the conventional and new methods for the detection of allergens in food are listed and briefly discussed.

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One-step multiplex PCR method for the determination of pecan and Brazil nut allergens in food products

Abstract: The presented multiplex PCR method is specific enough and can be used as a fast approach for the detection of major allergens of pecan or Brazil nuts in food.

Cited by 17 publications

References 32 publications

Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR

Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR

DNA Barcoding for Minor Crops and Food Traceability

Recent Advances in the Detection of Allergens in Foods

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