One-step lignocellulose fractionation using acid/pentanol pretreatment for enhanced fermentable sugar and reactive lignin production with efficient pentanol retrievability

Non-catalytic proteins as promising detoxifiers in lignocellulosic biomass pretreatment: unveiling the mechanism for enhanced enzymatic hydrolysis

“…In short, 10 g of oven-dried biomass powders were soaked in 100 mL of 1% H 2 SO 4 aqueous solution and heated in the autoclave at 120 °C for 60 min. 26 To mitigate the release of toxic inhibitors, after pretreatment, different doses (20,35,50,65, and 80 mg g −1 ) of non-catalytic proteins (AP, SP, and BSA) were added into the pretreated mixture and heated at 80 °C for 30 min. After incubation, the pretreated hydrolysate (liquid fraction) and solid substrates were separated by vacuum filtration.…”

“…This is related to not only the presence of lignin in the solid substrate, but also the formation of pseudo-lignin on the substrate surfaces, which could restrict the accessibility of cellulose and adsorb the enzymes through non-specific interactions. 14,36 To examine the effect of non-catalytic proteins on pretreated hydrolysate detoxification, the pretreated mixtures were post-incubated with different doses of AP, SP, and BSA (20,35,50,65, and 80 mg g −1 ), and their enzymatic digestibility experiments were subsequently carried out. The enzymatic digestibility yield was considerably enhanced with the increased doses of AP, SP, and BSA.…”

Construction of anhydrous two-step organosolv pretreatment of lignocellulosic biomass for efficient lignin membrane-extraction and solvent recovery

“…Fourier transform infrared (FT-IR) spectroscopy was performed on a NICOLET NEXUS 470 equipment (Thermo Nicolet, America) in a KBr tablet in the wavenumber range of 400-4000 cm −1 , and the number of scans was 128 [32]. The 2D-HSQC nuclear magnetic resonance (NMR) was conducted based on the previous literature [33].…”

Efficient catalytic oxidation of biomass to formic acid coupled with low-energy formaldehyde production from methanol

“…16,24,33 A strategy can be considered that lignin is first separated from lignocellulosic biomass for the preparation of high value-added aromatic compounds, 41 and the remaining saccharides are efficiently converted to FA via a coupling system. So far, many efficient methods for the component fractionation of lignocellulose have been reported, [41][42][43][44][45][46][47] providing feasibility for the fractionation-valorization strategy.…”