2013
DOI: 10.1038/srep02825
|View full text |Cite
|
Sign up to set email alerts
|

One-Step Instant Synthesis of Protein-Conjugated Quantum Dots at Room Temperature

Abstract: We present a new general facile strategy for the preparation of protein-functionalized QDs in a single step at ambient conditions. We demonstrated that highly luminescent red to near-infrared (NIR) protein-functionalized QDs could be synthesized at room temperature in one second through a one-pot reaction that proceeds in aqueous solution. Herein protein-functionalized QDs were successfully constructed for a variety of proteins with a wide range of molecular weights and isoelectric points. The as-prepared prot… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
75
0
1

Year Published

2015
2015
2021
2021

Publication Types

Select...
6
3

Relationship

0
9

Authors

Journals

citations
Cited by 90 publications
(76 citation statements)
references
References 31 publications
0
75
0
1
Order By: Relevance
“…18,19 The synthesis is carried out by reacting GST-EGFP-GB1 with lead acetate (Pb(Ac) 2 ) at ambient conditions to promote the binding of Pb 2+ to GST-EGFP-GB1 (see, experimental section in ESI). 18,19 The synthesis is carried out by reacting GST-EGFP-GB1 with lead acetate (Pb(Ac) 2 ) at ambient conditions to promote the binding of Pb 2+ to GST-EGFP-GB1 (see, experimental section in ESI).…”
mentioning
confidence: 99%
“…18,19 The synthesis is carried out by reacting GST-EGFP-GB1 with lead acetate (Pb(Ac) 2 ) at ambient conditions to promote the binding of Pb 2+ to GST-EGFP-GB1 (see, experimental section in ESI). 18,19 The synthesis is carried out by reacting GST-EGFP-GB1 with lead acetate (Pb(Ac) 2 ) at ambient conditions to promote the binding of Pb 2+ to GST-EGFP-GB1 (see, experimental section in ESI).…”
mentioning
confidence: 99%
“…In most biological applications the QDs have a top layer of ZnS increase the access of QDs to the molecular targets in in-vivo imaging [73]. Despite these advantages the ligand exchange process has several disadvantages:…”
Section: Ligand Exchange Processesmentioning
confidence: 99%
“…It should be noted that the mobility difference of free GOD and GOD-capped Mn-ZnS QDs was rather small. 9 Then, the chemically modified GOD was explored for the proposed on-step instant synthesis of Mn-ZnS QDs. On the basis of the functional groups in GOD, five categories can be identified, namely imidazole in His, hydroxyl groups in Tyr/Ser/Thr, carboxyl groups in Asp/Glu, amine groups in Lys, and thiol groups in Cys (Table 1).…”
Section: Investigation On the Role Of God In One-step Instant Synthesmentioning
confidence: 99%
“…In other words, the role of proteins is similar to that of thiols, which direct the growth of QDs as capping ligands. 9 Enzymes play prominent roles in biological metabolism systems owing to their high substrate-specificity and high catalytic efficiency. 6 Without these stringent conditions, large sized nanocrystals (larger than the Bohr radius) would be obtained and accordingly, losing the quantum confinement of QDs.…”
Section: Introductionmentioning
confidence: 99%