One-step-immunoassay of procalcitonin enables rapid and accurate diagnosis of bacterial infection

Oh, Joo Hyeong; Kwon, J.-H.; Kim, Hye-Hyun; Lee, Jeewon

doi:10.1039/d1ra02494a

Cited by 5 publications

(6 citation statements)

References 47 publications

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“…Its quantitative feature overcomes the limitations of standard qualitative LIFA tests, thereby enhancing the accuracy and reliability of HbA1c measurements [13,19]. With the ongoing developments in LIFA technology and continuous evaluation of various instruments, the clinical utility of HbA1c measurements using LIFA can be further enhanced [12,19].…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the primary focus of this study was to apply LIFA to measure HbA1c levels and contrast it with conventional techniques such as reversed-phase cation-exchange chromatography and HPLC. The underlying motivation for employing LIFA in this study is rooted in the demand for diagnostic methods that are more accessible, cost-effective, and precise, particularly for monitoring conditions like diabetes [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…However, HPLC techniques require skilled personnel with expertise in operating and maintaining sophisticated equipment. Analyzing the data obtained from these methods may also require the involvement of experienced analysts or scientists with a background in chromatography and related techniques [10,12].…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Evaluation of HbA1c Measurement Methods and Their Implications for Diabetes Management

Yun,

Park,

Kim

2023

Diagnostics

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In this study, we assessed the correlations between hemoglobin A1c (HbA1c) measurements obtained using three different diagnostic methods, namely reversed-phase cation-exchange chromatography, high-performance liquid chromatography, and lateral flow immunoassay (LIFA) with an AnyLab F instrument. HbA1c levels measured with the AnyLab F instrument and those measured with the HA8190V, HA8180, and D100 instruments were strongly correlated. High R-square values and low p-values indicated significant and reliable correlations, supporting the clinical interchangeability of these methods. Notably, demographic and clinical analyses revealed uniform HbA1c levels across age groups, suggesting minimal age-related variations in HbA1c levels in the cohort. This finding has implications for diabetes management strategies across different age groups, emphasizing the versatility of the AnyLab F instrument. Overall an average HbA1c level of 7.857% among diabetes mellitus-diagnosed participants suggests moderately elevated HbA1c levels, underscoring the need for improved diabetes management. Younger individuals exhibited lower HbA1c levels, potentially owing to heightened awareness and treatment plan adherence. Conversely, older adults had higher HbA1c levels, likely influenced by age-related changes and comorbidities. Larger sample sizes and a comprehensive evaluation of various measurement principles are needed to strengthen the findings herein. Additionally, exploring additional biomarkers and assessing LIFA performance in larger sample sets will advance the clinical utility of HbA1c measurements.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Comparative Evaluation of HbA1c Measurement Methods and Their Implications for Diabetes Management

Yun,

Park,

Kim

2023

Diagnostics

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“…Additionally, patients with HBV tend to have higher CRP levels than healthy individuals (Moura et al, 2019). Nevertheless, our understanding of the correlation between serum PCT levels and liver disorders such as liver cirrhosis and hepatocellular carcinoma resulting from HBV infection remains limited (Igna et al, 2022;Oh et al, 2021;Tan et al, 2018). These findings highlight the involvement of CRP in the inflammatory response and its potential correlation with the severity of HBV infection.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Potential of Inflammatory Biomarker Levels in the Serum of Patients with Hepatitis B Infection: A Retrospective Study

Yun,

Jung,

Rheem

et al. 2023

American Journal of Biochemistry and Biotechnology

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This study aimed to evaluate the diagnostic biomarker potential of C-Reactive Protein (CRP) and Procalcitonin (PCT) levels in the serum of individuals infected with the Hepatitis B Virus (HBV). The patients were divided into two age groups: Younger (<60 years) and older (≥60 years). An Electrochemiluminescence Immunoassay (ECLIA) platform was employed for the serological analysis to quantify the biomarker levels. A retrospective analysis was conducted on a cohort of HBV-infected patients and serum samples were collected from 200 individuals infected with HBV as well as from healthy controls. ECLIA revealed significantly increased levels of the pro-inflammatory markers CRP and PCT in both younger and older HBVinfected patients compared with those for participants in the healthy controls (p<0.0001). Importantly, the serum CRP and PCT levels were independent of age and sex. These findings provide evidence for increased serum CRP and PCT levels following HBV infection, indicating a potential correlation between these biomarkers and HBV infection. Thus, this study demonstrates the promising clinical diagnostic significance of CRP and PCT in HBV infections when assessed using ECLIA.

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“…Furthermore, HBV-ACLF patients have shown increased levels of both CRP and PCT . However, limited information is available regarding the correlation between serum PCT levels and dysfunction caused by HBV infection [12].…”

Section: Introductionmentioning

confidence: 99%

Inflammatory Dysfunction in Hepatitis B-Positive Individuals: Quantitative Assessment of C-reactive protein (CRP) and Procalcitonin (PCT) Levels Using Immunofluorescence Assay

Yun,

Park,

Min

et al. 2023

APJCRI

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This study successfully identifies CRP and PCT as robust and dependable diagnostic biomarkers for HBV infection in serum samples. Inflammatory dysfunction is commonly observed in various chronic illnesses, including severe liver disease caused by hepatitis. Clinical practice often relies on C-reactive protein (CRP) and procalcitonin (PCT) as the primary serological inflammatory markers. This study aimed to quantitatively analyze and evaluate serum CRP and PCT levels in younger (<60 years) and older (≥60 years) individuals infected with hepatitis B virus (HBV). A novel quantitative immunofluorescence assay (IFA) was used to evaluate CRP and PCT as potential diagnostic biomarkers for HBV infection. A retrospective analysis was conducted on 104 patients with HBV infection and 96 healthy controls. Serum CRP and PCT levels were measured using the quantitative IFA method. Between-group significance was determined using independent t-tests. Results showed significantly enhanced serum CRP and PCT levels in both younger and older patients with HBV infection than healthy controls (p<0.0001). Notably, CRP and PCT levels were independent of age and sex. Our study successfully identifies CRP and PCT as reliable diagnostic biomarkers for HBV infection in serum samples. Furthermore, The quantitative immunofluorescence assay (IFA) is being introduced as a novel and superior diagnostic technique, with several advantages over conventional lateral flow immunoassays. The quantitative IFA method demonstrates cost-effectiveness, rapidity, ease of use, and improved diagnostic capabilities. Beyond establishing these dependable biomarkers, our research introduces a genuinely promising diagnostic avenue. The potential applications in clinical settings are tangible, presenting a proactive shift toward improved HBV infection diagnosis and management. The significance of this contribution extends far beyond the confines of this study, paving the way for enhanced medical practices.

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One-step-immunoassay of procalcitonin enables rapid and accurate diagnosis of bacterial infection

Abstract: An advanced one-step-immunoassay enables quick and accurate diagnosis of sepsis with generation of clear optical signals in assay solution, which is based on sensitive detection of procalcitonin in patient sera.

Cited by 5 publications

References 47 publications

A Comparative Evaluation of HbA1c Measurement Methods and Their Implications for Diabetes Management

A Comparative Evaluation of HbA1c Measurement Methods and Their Implications for Diabetes Management

Diagnostic Potential of Inflammatory Biomarker Levels in the Serum of Patients with Hepatitis B Infection: A Retrospective Study

Inflammatory Dysfunction in Hepatitis B-Positive Individuals: Quantitative Assessment of C-reactive protein (CRP) and Procalcitonin (PCT) Levels Using Immunofluorescence Assay

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