One-step growth curves for vaccinia virus in cultures of monkey kidney cells

Furness, G.; Youngner, Julius S.

doi:10.1016/0042-6822(59)90130-8

Cited by 38 publications

(17 citation statements)

References 7 publications

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“…The resulting peptide solutions were applied to a filter comprising styrene divinylbenzene (SDB) copolymer modified with sulfonic acid (3M, Inc.) that had been conditioned with 0.1% NH 4 OH. After the filter was washed with 0.1% NH 4 OH, peptides were eluted from SDB in three fractions (10,20, and 80% CH 3 CN in 0.1% NH 4 OH) as described previously (27). The 500 mM salt cut was desalted using SDB and then eluted with 80% CH 3 CN only.…”

Section: Methodsmentioning

confidence: 99%

An Overview of the Vaccinia Virus Infectome: a Survey of the Proteins of the Poxvirus-Infected Cell

Chou

Ngo

Gershon

2012

J Virol

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We have quantitatively profiled the proteins of vaccinia virus-infected HEK293T cells early and late during vaccinia virus infection. Proteins corresponding to 4,326 accessions were identified, the products of 3,798 genes. One hundred thirty-six of the proteins were vaccinia virus-encoded (ϳ64% of the known vaccinia virus proteome). The remaining accessions were from the host cell. A total of 3,403 of the 4,326 accessions could be confidently quantitated at the precursor peptide level. Although vaccinia virus gene products spanned the entire abundance dynamic range of the cellular proteome, nearly all of the proteome dynamics observed as a result of infection were manifest in the virus gene products with very little plasticity in the host cell proteome. The vaccinia virus gene products could be grouped into four kinetic classes (i.e., four combinations of pre-and postreplicative expression). These protein kinetic classes reflected, almost entirely, the corresponding gene classes within the recently characterized vaccinia virus transcriptome map. The few cellular gene products that showed notable changes in abundance upon vaccinia virus infection were concentrated largely in just a few functional groups. After all of the quantitated cellular gene products were assigned to Gene Ontology (GO)-specific groups, quantitation values for a number of these GO-specific groups were significantly skewed toward over-or underabundance with respect to the global distribution of quantitation values. Quantitative analysis of host cell functions reflected several known facets of virus infection, along with some novel observations.

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Section: Methodsmentioning

confidence: 99%

An Overview of the Vaccinia Virus Infectome: a Survey of the Proteins of the Poxvirus-Infected Cell

Chou

Ngo

Gershon

2012

J Virol

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“…3) has been similarly reported in one-step growth curves of vaccinia virus (Furness and Youngner 1959) and other animal viruses (Gomatos et al 1962;Green 1962). A relationship between the exponential multiplication of ICV and the rise in the ECV titer has been suggested by Vogt (1953, 1954).…”

Section: Notes 93mentioning

confidence: 58%

“…Similar relationships between the duration of the latent phase and the MOI have been reported with other animal viruses (Dulbecco and Vogt 1954;Kaplan 1957). The length of the latent period varies from 5 to 20 hpi in animal viruses including vaccinia virus (Kaplan 1957;Furness and Youngner 1959;Gomatos et al 1962) to 68 hpi in YMTV (Yohn et al 1966). The free virus present during the latent period in this study (Fig.…”

mentioning

confidence: 54%

“…Single-step growth cycles of animal viruses have been well documented, and the duration of the replication cycle varies widely depending on the virus, its host, and the optimum temperature. The replication cycle of poxviruses varies from 24 h postinfection (hpi) in the cases of vaccinia virus (Furness and Youngner 1959;Salzman 1960) and rabbitpox virus (Appleyard and Westwood 1964) to 8 days in the case of YMTV. (Yohn et al 1966;Yohn et al 1970).…”

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confidence: 99%

“…The length of eclipse period is dependent on the cell strain, virus strain, and MOI (Darlington and Granoff 1973) and has been found to vary in poxviruses from 8-10 hpi in vaccinia virus (Furness and Youngner 1959), 4 hpi in rabbitpox virus (Appleyard and Westwood 1964), to 54 hpi in YMTV (Yohn et al 1966). The infective virus present during the eclipse phase represents the parent of the progeny virion (Furness and Youngner 1959), which may not have participated in the infection.…”

mentioning

confidence: 99%

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The replication cycle of tanapox virus in owl monkey kidney cells

Mediratta¹,

Essani²

1999

Can. J. Microbiol.

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The growth kinetics of tanapox virus in owl monkey kidney cells was elucidated by single-step growth curves at multiplicities of 10, 1.0, and 0.1 plaque forming units (pfu) per cell at 37 and 33 degrees C. Virus replicated equally well at both temperatures and produced a cytopathic effect that was characterized by densely packed rounded cells with retrogressed monolayer and granular vacuolated cytoplasm. Single-step growth curves revealed that the eclipse period varied from 24 h postinfection (hpi) at a multiplicity of infection of 10 pfu/cell to 48 hpi at 0.1 pfu/cell. The length of the latent period also varied from 36 hpi at 10 pfu/cell to 48 hpi at 0.1 pfu/cell. The intracellular virus, extracellular virus, and total virus titers reached their maximums relatively early at 10 pfu/cell as compared with 0.1 pfu/cell. About 78% of the mature progeny virion is retained intracellularly at 10 pfu/cell at 96 hpi. We conclude that tanapox virus replication is similar to other poxviruses, but the replication cycle is longer when compared with vaccinia virus.

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Das Vaccinevirus

Mayr¹,

Muñz²

1965

Handbuch Der Schutzimpfungen

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One-step growth curves for vaccinia virus in cultures of monkey kidney cells

Cited by 38 publications

References 7 publications

An Overview of the Vaccinia Virus Infectome: a Survey of the Proteins of the Poxvirus-Infected Cell

An Overview of the Vaccinia Virus Infectome: a Survey of the Proteins of the Poxvirus-Infected Cell

The replication cycle of tanapox virus in owl monkey kidney cells

Das Vaccinevirus

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