One-Step Extraction and Cleanup Procedure for Determination of p,p'-DDT, p,p'-DDD, and p,p'-DDE in Fish

Ahmad, Nazir; Marolt, Robert Slobodan

doi:10.1093/jaoac/69.4.581

Cited by 14 publications

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“…Organochlorine compounds were analysed by the ‘one‐step method’ of Ahmad and Marolt (1986). Concentrations of OC were determined for whole sediments and reported as ng/g (dry weight) of sediment.…”

Section: Methodsmentioning

confidence: 99%

Historical catchment changes and temporal impact on sediment of the receiving basin, Port Jackson, New South Wales

Taylor

Birch²,

Links

2004

Aust J Earth Sci

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Port Jackson (Sydney Harbour) is a major deep-water estuary on the central New South Wales coast and is surrounded by a highly urbanised and industrialised catchment. Concentrations of trace metals and organochlorine pesticide residues are high in sediment in the upper reaches of the estuary. Historical fluxes of contaminants into the estuary were determined by radio-isotopic dating of 12 sediment profiles in nine highly contaminated embayments. The onset of contamination in estuarine sediment was correlated with catchment-wide urbanisation and industrialisation of Sydney. Contamination of estuarine sediment in Port Jackson first occurred ca 1860, near the present central business district and spread westward and northward. Trace-metal contamination was initiated ~20 years after the commencement of industry in the subcatchments, and a rapid increase in trace-metal concentrations corresponds closely with maximum industrial activity in these catchments. Organochlorine pesticide residues occur in estuarine sediments 40-50 years after the onset of contamination by trace metals and their appearance closely corresponds with the manufacturing and application history of these compounds. The transit time of contaminants from fluvial catchment to incorporation in estuarine sediment is short (probably <2 years). Trace metals in sediment reached maximum concentrations in 1970 and a subsequent decrease in concentrations in the upper estuary reflects relocation of industry away from the estuary foreshores and tighter controls on industrial discharges. In Middle Harbour and the lower estuary, trace-metal concentrations continue to increase, due to the comparatively recent increases in commercial and industrial growth in these areas.

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Section: Methodsmentioning

confidence: 99%

Historical catchment changes and temporal impact on sediment of the receiving basin, Port Jackson, New South Wales

Taylor

Birch²,

Links

2004

Aust J Earth Sci

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“…Traditional methods for determination of chlorinated pesticides in tissue samples involve solvent extraction, partitioning of the pesticide residues from fat into another liquid, and cleanup of the extract to remove coextractives prior to gas chromatography. Consequently, the methods are lengthy and labor-intensive and are therefore not suitable for the analysis of the large number of samples required for comprehensive studies (Ahmad and Marolt, 1986;Herna ´ndez et al, 1993;Bucholski et al, 1996;Menezes and Felix, 1996).…”

Section: Introductionmentioning

confidence: 99%

Hapten Synthesis and Production of Monoclonal Antibodies to DDT and Related Compounds

Abad

Manclús

Mojarrad

et al. 1997

J. Agric. Food Chem.

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This work describes the production and characterization of monoclonal antibodies (MAbs) to the organochlorine insecticide DDT and their incorporation into several ELISA configurations. A collection of DDT haptens was synthesized by introducing appropriate spacers at two sites of the analyte molecular structure. From mice immunized with hapten−protein conjugates, MAbs with I 50 values to p,p‘-DDT in the 2−11 nM range in homologous conjugate-coated assays were obtained. According to their cross-reactivity pattern with DDT isomers and metabolites, MAbs can be classified as class-specific or DDT-specific antibodies. Both types of MAbs were obtained from mice immunized with the same hapten−protein conjugate simply by applying a different selection criterion in the screening of fusion supernatants. These immunoassays are potentially very valuable analytical tools for the rapid and sensitive determination of DDT and congeners in food and the environment and for monitoring human exposure to these ubiquitous and toxic compounds. Keywords: ELISA; insecticide; organochlorine pesticide; monoclonal antibodies; DDT; DDE; DDD; hapten design; hapten heterology; ELISA format

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“…The extraction and analyses of organochlorine compounds in sediments were based on the ‘one‐step method’ of Ahmad and Marolt (1986). Concentrations of organochlorines were determined from wet sediments and reported as ng g –1 dry weight of sediment.…”

Section: Methodsmentioning

confidence: 99%

Distribution and possible sources of organochlorine residues in sediments of a large urban estuary, Port Jackson, Sydney

Birch

Taylor

2000

Australian Journal of Earth Sciences

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Sydney Harbour is surrounded by a large capital city of about four million people and its highly urbanised (86%) catchment supports a substantial industrial base and an extensive transport infrastructure. Large commercial and naval ports occupy the waterway and the harbour is an important recreational area. Surficial sediment in Sydney Harbour contains high concentrations of PCBs, HCB, total chlordane, total DDT, aldrin, dieldrin, heptachlor and heptachlor‐epoxide, but low concentrations of lindane. PCBs, total chlordane, and to a lesser extent dieldrin, are most elevated in sediment in creeks on the southern shores of the harbour suggesting sources within older, highly urbanised/industrialised catchments of western‐central Sydney. There are high concentrations of total DDT and HCB in sediments of the upper harbour and Homebush Bay suggesting that chemical industries on the shores of the estuary in this area are sources of these contaminants. Although no sediment quality guidelines apply in Australia, empirically derived biological effects criteria suggest that sediment over extensive areas of Sydney Harbour may have an adverse impact on biota. Especially of concern are sediments containing high concentrations of chlordane and DDT.

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One-Step Extraction and Cleanup Procedure for Determination of p,p'-DDT, p,p'-DDD, and p,p'-DDE in Fish

Cited by 14 publications

References 0 publications

Historical catchment changes and temporal impact on sediment of the receiving basin, Port Jackson, New South Wales

Historical catchment changes and temporal impact on sediment of the receiving basin, Port Jackson, New South Wales

Hapten Synthesis and Production of Monoclonal Antibodies to DDT and Related Compounds

Distribution and possible sources of organochlorine residues in sediments of a large urban estuary, Port Jackson, Sydney

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