One-Step Electrochemical Sensing of CA-125 Using Onion Oil-Based Novel Organohydrogels as the Matrices

Abstract

To reduce the high mortality rates caused by ovarian cancer, creating high-sensitivity, quick, basic, and inexpensive methods for following cancer antigen 125 (CA-125) levels in blood tests is of extraordinary significance. CA-125 is known as the exclusive glycoprotein employed in clinical examinations to monitor and diagnose ovarian cancer and detect its relapses as a tumor marker. Elevated concentrations of this antigen are linked to the occurrence of ovarian cancer. Herein, we designed organohydrogels (ONOHs) for identifying the level of CA-125 antigen at fast and high sensitivity with electrochemical strategies in a serum medium. The ONOH structures are synthesized with glycerol, agar, and glutaraldehyde and at distinct ratios of onion oil, and then, the ONOHs are characterized with Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). Electrochemical measurements are performed by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) in the absence and presence of CA-125 on the designed ONOHs. For the prepared ONOH-3 electrode, two distinct linear ranges are determined as 0.41−8.3 and 8.3−249.0 U/mL. The limit of quantitation and limit of detection values are calculated as 2.415 and 0.805 μU/mL, respectively, (S/N = 3). These results prove that the developed electrode material has high sensitivity, stability, and selectivity for the detection of the CA-125 antigen. In addition, this study can be reasonable for the practical detection of CA125 in serum, permitting early cancer diagnostics and convenient treatment.