One-step continuous/semi-continuous whole-cell catalysis production of glycolic acid by a combining bioprocess with in-situ cell recycling and electrodialysis

Hua, Xia; Cao, Rou; Zhou, Xin; Xu, Yong

doi:10.1016/j.biortech.2018.11.061

Cited by 31 publications

(17 citation statements)

References 18 publications

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“…The pressure and the oxygen consumption rate of microorganisms in the bioreactor directly determined the oxygen inflow rate. Foaming was monitored by on-line addition of polyethylene as an antifoam agent in AS-BR and OS-BR, but not in SOS-BR [41].…”

Section: Whole-cell Catalysismentioning

confidence: 99%

Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS)

Hua

Zhou

et al. 2020

Biotechnol Biofuels

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156

168

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Background: The critical issue in the competitiveness between bioengineering and chemical engineering is the products titer and the volume productivity. The most direct and effective approach usually employs high-density biocatalyst, while the weakened mass transfer and evoked foam problem accompany ultrahigh-density biocatalyst loading and substrate/product titer. In high-density obligate aerobic bioconversion, oxygen as electron acceptor is a speed-limiting step in bioprocesses, but sufficient oxygen supply will lead to the foaming which results in a significant reduction in oxygen utilization and the use of additional defoamers. In this study, we designed a novel sealed-oxygen supply (SOS) biotechnology to resolve the formidable barrier of oxygen transferring rate (OTR), for bio-based fuels and chemical production process. Results: Based on systemic analysis of whole-cell catalysis in Gluconobacter oxydans, a novel sealed-oxygen supply technology was smartly designed and experimentally performed for biocatalytic oxidation of alcohols, sugars and so on. By a simple operation skill of automatic online supply of oxygen in a sealed stirring tank bioreactor of SOS, OTR barrier and foaming problem was resolved with great ease. We finally obtained ultrahigh-titer products of xylonic acid (XA), 3-hydroxypropionic acid (3-HPA), and erythrulose at 588.4 g/L, 69.4 g/L, and 364.7 g/L, respectively. Moreover, the volume productivity of three chemical products was improved by 150-250% compared with normal biotechnology. This SOS technology provides a promising approach to promote bioengineering competitiveness and advantages over chemical engineering. Conclusion: SOS technology was demonstrated as an economic and universally applicable approach to bio-based fuels and chemicals production by whole-cell catalysis. The novel technology greatly promotes the competitiveness of bioengineering for chemical engineering, and provides a promising platform for the green and environmental use of biofuels.

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Section: Whole-cell Catalysismentioning

confidence: 99%

Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS)

Hua

Zhou

et al. 2020

Biotechnol Biofuels

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156

168

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“…Glycolic acid (GA) is the simplest alpha-hydroxy acid (Hua et al 2019), pKa 3.83. Due to its strong chemical activity, GA is widely used as a biosynthetic of glycolic acid from glycolonitrile with a new bacterial isolate (He et al 2010;Shi et al 2006).…”

Section: Glycolic Acidmentioning

confidence: 99%

The challenges and prospects of Escherichia coli as an organic acid production host under acid stress

Yang

Zhang

Zhu

et al. 2021

Appl Microbiol Biotechnol

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Objective Organic acids have a wide range of applications and have attracted the attention of many industries, and their large-scale applications have led fermentation production to low-cost development. Among them, the microbial fermentation method, especially using Escherichia coli as the production host, has the advantages of fast growth and low energy consumption, and has gradually shown better advantages and prospects in organic acid fermentation production. Importance However, when the opportunity comes, the acidified environment caused by the acid products accumulated during the fermentation process also challenges E. coli. The acid sensitivity of E. coli is a core problem that needs to be solved urgently. The addition of neutralizers in traditional operations led to the emergence of osmotic stress inadvertently, the addition of strong acid substances to recover products in the salt state not only increases production costs, but the discharged sewage is also harmful to the environment. Elaboration This article summarizes the current status of the application of E. coli in the production of organic acids, and based on the impact of acid stress on the physiological state of cells and the impact of industrial production profits, put forward some new conjectures that can make up for the deficiencies in existing research and application. Implication At this point, the diversified transformation of E. coli has become a chassis microbe that is more suitable for industrial fermentation, enhancing industrial application value. Key points • E. coli is a potential host for high value-added organic acids production.• Classify the damage mechanism and coping strategies of E. coli when stimulated by acid molecules.• Multi-dimensional expansion tools are needed to create acid-resistant E. coli chassis.

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“…The prerequisite for the feasibility of preparing BA using aerobic microorganisms is to surmount the problem of biomass-based butanol volatilization. Because G. oxydans is unable to metabolize biomass-based butanol for growth, it means that the whole-cell catalysis process occurs without any CO 2 being produced [25]. Based on the fact, we designed an SOS-BR which can achieve bioreactor sealing and pressurization.…”

Section: The Whole-catalysis Of Biomass-based Butanol In Sos-brmentioning

confidence: 99%

A techno-practical method for overcoming the biotoxicity and volatility obstacles of butanol and butyric acid during whole-cell catalysis by Gluconobacter oxydans

Hua

Zhou

et al. 2020

Biotechnol Biofuels

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Background: Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality butyric acid used in food and medicine, is more dependent on microbiological production methods. Hence, the bio-oxidation of butanol to butyric acid has been identified as a promising method with good potential economic and environmental benefits. However, both butanol and butyric acid are usually intensively toxic to most microorganisms as well as the bio-oxidation pathway. To develop a green, efficient and competitive microbiological method is the primary work to overcome the bottleneck of butyric acid industry. Result: A combined bioprocess was designed with alternative whole-cell catalysis for butyric acid bio-conversion from butanol by Gluconobacter oxydans in a sealed-oxygen supply bioreactor (SOS). In the operation system, the escape of volatile substrates and toxic chemicals to cells can be avoided by the use of a sealed bioreactor, combined with the rejuvenation of cells by supplying energy co-factors. Finally, during a one-batch whole-cell catalysis, the utilization rate of substrate increased from 56.6 to 96.0% by the simple skill. Additionally, the techno-practical bioprocess can realize the purpose of cell-recycling technology through the rejuvenation effect of co-factor. Finally, we obtained 135.3 g/L butyric acid and 216.7 g/L sorbose during a 60-h whole-cell catalysis. This techno-practical technology provides a promising approach to promote the industrial production of butyric acid with more competitiveness. Conclusion: The techno-practical biotechnology has powerfully promoted the process of butyric acid production by microorganisms, especially makes up for the lack of aerobic fermentation in the industry, and surmounts the shortcomings of traditional anaerobic fermentation. At the same time, this technically practical system provides a promising approach for the promotion of the industrial production of butyric acid in a more competitive manner.

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One-step continuous/semi-continuous whole-cell catalysis production of glycolic acid by a combining bioprocess with in-situ cell recycling and electrodialysis

Cited by 31 publications

References 18 publications

Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS)

Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS)

The challenges and prospects of Escherichia coli as an organic acid production host under acid stress

A techno-practical method for overcoming the biotoxicity and volatility obstacles of butanol and butyric acid during whole-cell catalysis by Gluconobacter oxydans

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