One-step competitive immunochromatographic assay for semiquantitative determination of lipoprotein(a) in plasma

Lou, Sheng C.; Patel, Chandu; Ching, Simon; Gordon, J.

doi:10.1093/clinchem/39.4.619

Cited by 83 publications

(27 citation statements)

References 23 publications

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“…A huge amount of assays have been developed on the capillary test strip platform during the past 30 years. 75 Today, they serve a wide field of applications, including health biomarkers (pregnancy, 13,76 heart attack, 70 blood glucose, 77 metabolic disorders 78 ), small molecules (drug abuse, 16 toxins, 79 antibiotics 80 ), infectious agents (anthrax, 81 salmonella, 82 viruses 83 ), immunodiagnostics, 84 RNA applications, 81 and even whole bacteria. 85 Some of the more recent designs and publications even show the detection of DNA 83 without the need of amplification by PCR, which would open yet another vast field of new applications.…”

Section: Application Examplesmentioning

confidence: 99%

Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications

et al. 2010

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This critical review summarizes developments in microfluidic platforms that enable the miniaturization, integration, automation and parallelization of (bio-)chemical assays (see S. Haeberle and R. Zengerle, Lab Chip, 2007, 7, 1094-1110, for an earlier review). In contrast to isolated application-specific solutions, a microfluidic platform provides a set of fluidic unit operations, which are designed for easy combination within a well-defined fabrication technology. This allows the easy, fast, and cost-efficient implementation of different application-specific (bio-)chemical processes. In our review we focus on recent developments from the last decade (2000s). We start with a brief introduction into technical advances, major market segments and promising applications. We continue with a detailed characterization of different microfluidic platforms, comprising a short definition, the functional principle, microfluidic unit operations, application examples as well as strengths and limitations of every platform. The microfluidic platforms in focus are lateral flow tests, linear actuated devices, pressure driven laminar flow, microfluidic large scale integration, segmented flow microfluidics, centrifugal microfluidics, electrokinetics, electrowetting, surface acoustic waves, and dedicated systems for massively parallel analysis. This review concludes with the attempt to provide a selection scheme for microfluidic platforms which is based on their characteristics according to key requirements of different applications and market segments. Applied selection criteria comprise portability, costs of instrument and disposability, sample throughput, number of parameters per sample, reagent consumption, precision, diversity of microfluidic unit operations and the flexibility in programming different liquid handling protocols (295 references).

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Section: Application Examplesmentioning

confidence: 99%

Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications

et al. 2010

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“…Another analytical format that might predict the probability of CHD incidence on site has been constructed to detect lipoprotein (a), a minor lipoprotein fraction, based on immunochromatography (Lou et al, 1993). This assay was developed by utilizing the principle of competitive binding between the native analyte and a labeled analyte to an immobilized antibody on an NC membrane, which differs from the enzyme assay for cholesterol, as shown earlier.…”

Section: Application To Lipoprotein Mixturementioning

confidence: 99%

Immunochromatographic membrane strip assay system for a single-class plasma lipoprotein cholesterol, exemplified by high-density lipoprotein cholesterol measurement

Paek

Jang

Mok

et al. 1999

Biotechnol. Bioeng.

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In assessing risk factors of coronary heart disease, a membrane immunochromatographic system that minimizes requirements of instrument and reagent handling was investigated by utilizing high‐density lipoprotein (HDL) cholesterol (HDL‐C) as model analyte. The system is composed of four functional membrane strip pads connected in sequence as follows (from the bottom): immunoseparation based on the biotin–streptavidin reaction; catalytic conversion of cholesterol to hydrogen peroxide; production of a colorimetric signal; and induction of a continuous wicking of medium. For immunochromatography, a monoclonal antibody, specific to apolipoprotein B100 that is present on the surfaces of low‐density lipoproteins (LDL) and very low‐density lipoproteins (VLDL), with a high binding constant (5 × 1010 L/mol), was raised and chemically conjugated to streptavidin. The conjugate was first reacted with lipoprotein particles, and this mixture was absorbed by the capillary action into the biotin pad of the system. After being transferred by medium, immunocapture of LDL and VLDL particles onto the biotin pad took place, and in situ generation of a colorimetric signal in proportion to HDL‐C occurred consecutively. The capture was selective as well as effective (minimum 88% of LDL and VLDL in clinical concentration ranges), and the detection limit of the HDL‐C was far lower than 20 mg per 100 mL. The same concept may also be applicable to LDL cholesterol measurement provided suitable antibodies specific to HDL and VLDL are available. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 62: 145–154, 1999.

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“…Lateral‐flow immunochromatographic assay (LFIA) is a simple, fast‐responding, and inexpensive technique that is useful for disease diagnosis, home testing, food safety evaluation, and the detection of various environmental and agricultural contaminants . Antibody‐labeled nanoparticles such as magnetic nanoparticles (MNPs), colloidal gold, selenium, colloidal carbon, quantum dots, upconverting phosphors, and liposomes are used to detect the presence of the target analytes. Among the particles mentioned above, magnetic LIFA is a classic, chemically stable, and widely used rapid response system.…”

Section: Introductionmentioning

confidence: 99%

MnFe₂O₄ nanoclusters as labels for the quantitative detection of D‐dimer in a lateral‐flow immunochromatographic assay

Wang

Tang

et al. 2018

J Chinese Chemical Soc

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In this study, MnFe 2 O 4 nanoclusters were prepared as bioprobes to establish a lateral-flow immunochromatographic assay (LFIA) for the rapid and quantitative detection of D-dimer for the first time. The magnetic properties of the magnetic labels play a key role in the quantitative detection of biomolecules. The 47.3-nm MnFe 2 O 4 magnetic nanoclusters (MNCs) with good dispersion and high saturation magnetization (76 emu/g) were fabricated via thermal decomposition of Fe(acac) 3 with Mn(acac) 2 . The prepared MnFe 2 O 4 MNCs were well dispersed in water because the surfaces were fully covered with 3,4-dihydroxyhydrocinnamic acid (DHCA) molecules by ligand exchange. Anti-D-dimer antibodies were coupled on the surface of MnFe 2 O 4 MNCs, and the target protein, D-dimer, was detected, in the range 0.05-6 μg/mL. This assay provides a promising platform for D-dimer detection for point-of-care diagnosis.

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One-step competitive immunochromatographic assay for semiquantitative determination of lipoprotein(a) in plasma

Cited by 83 publications

References 23 publications

Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications

Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications

Immunochromatographic membrane strip assay system for a single-class plasma lipoprotein cholesterol, exemplified by high-density lipoprotein cholesterol measurement

MnFe₂O₄ nanoclusters as labels for the quantitative detection of D‐dimer in a lateral‐flow immunochromatographic assay

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One-step competitive immunochromatographic assay for semiquantitative determination of lipoprotein(a) in plasma

Cited by 83 publications

References 23 publications

Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications

Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications

Immunochromatographic membrane strip assay system for a single-class plasma lipoprotein cholesterol, exemplified by high-density lipoprotein cholesterol measurement

MnFe2O4 nanoclusters as labels for the quantitative detection of D‐dimer in a lateral‐flow immunochromatographic assay

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MnFe₂O₄ nanoclusters as labels for the quantitative detection of D‐dimer in a lateral‐flow immunochromatographic assay