One-Stage Rumen Fistulation With Permanent Silicone Cannula in Sheep

Girardi, Annita Morais; Sousa, Samuel Santos; Sabes, Amanda Festa; Bueno, Gabriela; Módolo, Tiago José Caparica; Bonacin, Yuri da Silva; Pimenta, Beatriz de Assis; Marques, José António; Marques, Luiz Carlos

doi:10.3738/21751463.2140

Cited by 3 publications

(6 citation statements)

References 4 publications

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“…All animals ate immediately after surgery when presented with hay, regardless of surgical treatment. This is in agreement with previous observations 2,5,10 . However, mean postoperative DMI was lower in the two‐step treatment group, declining steadily over time and failing to reach preoperative intake levels at the end of the experiment as was observed in the one‐step group (Figure 4A).…”

Section: Discussionsupporting

confidence: 92%

“…This is in agreement with previous observations. 2,5,10 However, mean postoperative DMI was lower in the two-step treatment group, declining steadily over time and failing to reach preoperative intake levels at the end of the experiment as was observed in the one-step group ( Figure 4A). A fairly dramatic (P = .01) dip (−0.72 kg) in two-step treatment DMI was also noted on day 9, corresponding to the day of clamp removal and initial cannula fitting.…”

Section: Discussionmentioning

confidence: 62%

“…Two of the major surgical methods used by veterinary surgeons for rumen cannulation are the one‐step and two‐step techniques. The one‐step surgical procedure involves making a circular incision in the left paralumbar fossa and a subsequent incision in the rumen wall 3‐5 . The rumen is sutured to the abdominal wall to form a fistula into which a rubber or silicone cannula is then placed immediately after the procedure 4,5 .…”

Section: Introductionmentioning

confidence: 99%

“…The onestep surgical procedure involves making a circular incision in the left paralumbar fossa and a subsequent incision in the rumen wall. [3][4][5] The rumen is sutured to the abdominal wall to form a fistula into which a rubber or silicone cannula is then placed immediately after the procedure. 4,5 In the two-step procedure, a portion of the rumen is externalized through a vertical incision in the left paralumbar fossa and held with a custom-made clamp to occlude blood flow and promote adhesion of the rumen to the incision edges of the abdominal wall.…”

Section: Introductionmentioning

confidence: 99%

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Influence of two rumen cannulation techniques on postoperative recovery in sheep

2020

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Objective: To determine the influence of two methods of rumen cannulation on postoperative animal welfare. Study design: Experimental study. Animals: Twelve commercial wethers (n = 6 per group). Methods: Group 1 wethers underwent a one-step procedure to place a cannula immediately after fistulation of the rumen to the skin. Group 2 wethers underwent a two-step procedure in which a portion of the rumen was externalized and held with a clamp for 9 days, after which the cannula was placed into the fistula created by removal of the clamped rumen tissue. Feed intake and vital signs were monitored daily for 24 days postoperatively. Plasma fibrinogen and serum cortisol were measured daily for 14 days postoperatively to estimate inflammatory and stress responses, respectively. Change in body weight was also assessed. Results: Cannulation method did not affect (P > .05) body weight, temperature, heart rate, respiratory rate, fibrinogen level, or cumulative cortisol level. Feed intake was lower (0.82 vs 1.2 kg/d; P < .0001), and mean cortisol level was greater (124.2 vs 121.5 ng/mL; P = .038) in group 2 compared with group 1. Conclusion: Although both cannulation methods mostly elicited similar physiological responses, animals seemed to experience more discomfort and stress when undergoing the two-step procedure. Clinical significance: Rumen cannulation performed in one step is recommended to improve postoperative welfare.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of two rumen cannulation techniques on postoperative recovery in sheep

2020

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“…Initially, the animals were identified, treated for internal and external parasites and, housed in a tie-stall barn with a concrete floor and equipped with water and feed troughs. Thirty days prior the beginning of the experiment, the rumen cannulation was performed in a one-stage operation as described by Girard et al [ 22 ]. An incision was made in the skin of the left flank, in the dorsoventral direction, approximately 5 cm ventral to the transverse process of the lumbar vertebrae, and 8 cm caudal to the 13th rib.…”

Section: Methodsmentioning

confidence: 99%

Feed intake, nutrient digestibility, and selected rumen parameters in feedlot bulls fed diets with different feed additives

et al. 2021

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An experiment was conducted to evaluate the feed intake, nutrient digestibility and selected rumen parameters in feedlot bulls fed diets containing different feed additives. Six rumen-cannulated Nellore bulls (age = 8 ± 1.0 months; initial BW = 225 ± 13.2 kg) were distributed in a 6 × 6 Latin square design. Six experimental diets based on 30% corn silage and 70% concentrate on a dry matter (DM) basis were evaluated. Diets differed in feed additive on a DM basis, as follows: 1.4% bicarbonate and magnesium oxide in 3:1 ratio (BOX); 36 ppm lasalocid sodium (LAS); 30 ppm monensin sodium (MON); 25 ppm virginiamycin (VIR); 30 ppm monensin sodium plus 25 ppm virginiamycin (MV); and 3.15% commercial mineral supplement containing D-limonene and exogenous α-amylase (EOA). The experiment lasted 144 d, with six periods of 24 d. Each period consisted of 14 d for dietary adaptation, 3 d for feces and urine collection, and 7 d for omasal and ruminal digesta collection. Bulls fed the BOX diet showed greater (P < 0.05) intake of DM, organic matter (OM), neutral detergent fiber (apNDF), crude protein (CP), and starch compared to the other diets. Diets with LAS, MON, VIR, MV, or EOA did not influence (P > 0.05) the DM, OM, apNDF, CP, or starch intake of feedlot bulls. Bulls fed the EOA diet showed greater (trend; P = 0.09) ruminal digestibility of starch compared to the other diets. The feed additives did not affect (P > 0.05) the intestinal or total tract digestibility of starch, rumen pH, microbial efficiency, total rumen fluid, dilution rate, rate of intake, rate of degradation, or passage rate of the DM, OM, apNDF, and starch. In conclusion, LAS, MON, VIR, MV, and EOA diets reduced nutrient intake compared to BOX. Although all feed additives presented similar effects on rumen pH, temperature, and kinetics the presence of exogenous α-amylase in the EOA diet may increase ruminal starch digestibility and apparent total tract digestibility of DM and OM.

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The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

Cao

Zhu

et al. 2021

Front. Vet. Sci.

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This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult Hu sheep (48.8 ± 0.23 kg, 0.5 years) were fed at 0700 and 1,800 with ad libitum access to water. The rumen fistula was installed in the same batch from 0600 to 0900. Monitoring the dry mater intake and the output of dry mater faces 1, 2, 4, 6, 8, 10, 12, 14 days after fistulated surgery. The collection of rumen fluid was arranged at 1d during rumen surgery (DRS1), 3d after rumen surgery (ARS3), and 14d after rumen surgery (ARS14) for volatile fatty acid (VFA) and DNA extraction for sequencing and bioinformatics analysis. There was no difference in DMI, the pH apparent digestibility of dry matter, organic matter, neutral detergent fiber, acid detergent fiber both before and 14 days after surgery. Increases were observed in the acetate and total VFA at ARS3. There was no difference in digestion of dry material, organic material, neutral detergent fiber, and acid detergent fiber before and after surgery. The relative abundance of Bacteroides decreased from 61.96% at DRS1 to 28.85% at ARS3. In comparison with the DRS1 and ARS3, the relative abundance of Firmicutes in the ARS14 increased to 44.58% (P < 0.01). Proteobacteria increased from 11.33% at DRS1 to 51.66% at ARS3 and then decreased to 11.39% at ARS14. Prevotella decreased form 61.06% at DRS1 to 28.04% in the ARS3. Succinivibrio increased from 8.32% at DRS1 to 48.58% at ARS3, but decreased to 10.43% in the ARS14. Compared with DRS1 and ARS3, the ARS14 was higher in the Simpson and Shannon index. As for the BugBase function prediction, rumen fistula surgery increased the microorganism abundance of aerobic and facultative anaerobic phenotype, and anaerobic phenotype was decreased in the ARS3. There was higher microorganism abundance of aerobic phenotype in the ARS14 than before fistula installation. In conclusion, the rumen fistula surgery destroys the anaerobic environment of rumen, leading to differences in rumen microbial diversity and function, but the apparent digestibility and total VFA were not affected.

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One-Stage Rumen Fistulation With Permanent Silicone Cannula in Sheep

Cited by 3 publications

References 4 publications

Influence of two rumen cannulation techniques on postoperative recovery in sheep

Influence of two rumen cannulation techniques on postoperative recovery in sheep

Feed intake, nutrient digestibility, and selected rumen parameters in feedlot bulls fed diets with different feed additives

The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

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