One-Shot Single-Cell Proteome and Metabolome Analysis Strategy for the Same Single Cell
Jie Wu,
Qin-Qin Xu,
Yi-Rong Jiang
et al.
Abstract:Characterizing the profiles of proteome and metabolome at the single-cell level is of great significance in single-cell multiomic studies. Herein, we proposed a novel strategy called one-shot single-cell proteome and metabolome analysis (scPMA) to acquire the proteome and metabolome information in a single-cell individual in one injection of LC-MS/MS analysis. Based on the scPMA strategy, a total workflow was developed to achieve the single-cell capture, nanoliter-scale sample pretreatment, one-shot LC injecti… Show more
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