One-pot synthesis of trypsin-based magnetic metal–organic frameworks for highly efficient proteolysis

Zhong, Chao; Lei, Zhixian; Huang, Huan; Zhang, Mingyue; Cai, Zongwei; Lin, Zian

doi:10.1039/c9tb02315a

Cited by 18 publications

(11 citation statements)

References 48 publications

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“…131 These include HRP@GOx@HKUST-1@ Fe 3 O 4 , 33 Fe 3 O 4 /Ni-BTC@SAMS, 132 lipase@Fe 3 O 4 @MOF, 133 and iron oxide@ZIF-8@trypsin. 134 The magnetic support greatly promotes the separation and recovery of the catalyst, which is especially true for catalysts with nanoscale sizes.…”

Section: Main Advantages Of Enzyme/mofsmentioning

confidence: 99%

“…Moreover, in some cases, the utilization of magnetic Fe 3 O 4 nanoparticles for the fabrication of enzyme/MOF composites facilitates the reuse of immobilized enzymes . These include HRP@GOx@HKUST-1@Fe 3 O 4 , Fe 3 O 4 /Ni-BTC@SAMS, lipase@Fe 3 O 4 @MOF, and iron oxide@ZIF-8@trypsin . The magnetic support greatly promotes the separation and recovery of the catalyst, which is especially true for catalysts with nanoscale sizes.…”

Section: Main Advantages Of Enzyme/mofs and Enzyme/cofsmentioning

confidence: 99%

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Recent Advances in Emerging Metal– and Covalent–Organic Frameworks for Enzyme Encapsulation

Wang

Liao

2021

ACS Appl. Mater. Interfaces

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Enzyme catalysis enables complex biotransformation to be imitated. This biomimetic approach allows for the application of enzymes in a variety of catalytic processes. Nevertheless, enzymes need to be shielded by a support material under challenging catalytic conditions due to their intricate and delicate structures. Specifically, metal−organic frameworks and covalent−organic frameworks (MOFs and COFs) are increasingly popular for use as enzyme-carrier platforms because of their excellent tunability in structural design as well as remarkable surface modification. These porous organic framework capsules that host enzymes not only protect the enzymes against harsh catalytic conditions but also facilitate the selective diffusion of guest molecules through the carrier. This review summarizes recent progress in MOF-enzyme and COF-enzyme composites and highlights the pore structures tuned for enzyme encapsulation. Furthermore, the critical issues associated with interactions between enzymes and pore apertures on MOF-and COF-enzyme composites are emphasized, and perspectives regarding the development of high-quality MOF and COF capsules are presented.

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Section: Main Advantages Of Enzyme/mofsmentioning

confidence: 99%

Section: Main Advantages Of Enzyme/mofs and Enzyme/cofsmentioning

confidence: 99%

Recent Advances in Emerging Metal– and Covalent–Organic Frameworks for Enzyme Encapsulation

Wang

Liao

2021

ACS Appl. Mater. Interfaces

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“…Zhong et al used a simple one-pot method to synthesize a novel magnetic MOF with a large specific surface area and high enzyme-carrying capacity. The activity of immobilized trypsin was 2.6 times higher than that of the soluble enzyme, and the composite could be easily magnetically recovered [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Magnetic Metal–Organic Frameworks Functionalized by Ionic Liquid as Supports for Immobilization of Pancreatic Lipase

Dai

et al. 2022

Molecules

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Enzymes are difficult to recycle, which limits their large-scale industrial applications. In this work, an ionic liquid-modified magnetic metal–organic framework composite, IL-Fe3O4@UiO-66-NH2, was prepared and used as a support for enzyme immobilization. The properties of the support were characterized with X-ray powder diffraction (XRD), Fourier-transform infrared (FTIR) spectra, transmission electron microscopy (TEM), scanning electronic microscopy (SEM), and so on. The catalytic performance of the immobilized enzyme was also investigated in the hydrolysis reaction of glyceryl triacetate. Compared with soluble porcine pancreatic lipase (PPL), immobilized lipase (PPL-IL-Fe3O4@UiO-66-NH2) had greater catalytic activity under reaction conditions. It also showed better thermal stability and anti-denaturant properties. The specific activity of PPL-IL-Fe3O4@UiO-66-NH2 was 2.3 times higher than that of soluble PPL. After 10 repeated catalytic cycles, the residual activity of PPL-IL-Fe3O4@UiO-66-NH2 reached 74.4%, which was higher than that of PPL-Fe3O4@UiO-66-NH2 (62.3%). In addition, kinetic parameter tests revealed that PPL-IL-Fe3O4@UiO-66-NH2 had a stronger affinity to the substrate and, thus, exhibited higher catalytic efficiency. The results demonstrated that Fe3O4@UiO-66-NH2 modified by ionic liquids has great potential for immobilized enzymes.

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“…The resultant composite is much heavier and could disperse in water medium well, facilitating the attachment with the enzyme. The magnetic COFs can be recycled by an external magnetic elds and reduce the aggregation of lipase and in uence of methanol, [41][42][43][44] which lays a good foundation for its e cient industrial production of biodiesel. Here, we reported core-shell magnetic COFs (Fe 3 O 4 @COF-OMe), in which Fe 3 O 4 nanoparticles are wrapped by COF-shell.…”

Section: Introductionmentioning

confidence: 99%

Magnetic COFs as Satisfied Support for Lipase Immobilization and Recovery to Effectively Achieve the Production of Biodiesel by Great Maintenance of Enzyme Activity

Zhou

Cai

Xing

et al. 2021

Preprint

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Background: Production of biodiesel from renewable sources such as inedible vegetable oils by enzymatic catalysis has been a hotspot but remains a challenge on the efficient use of an enzyme. COFs (Covalent Organic Frameworks) with large surface area and porosity can be applied as ideal support to avoid aggregation of lipase and methanol. However, the naturally low density limits its application. In this work, we reported a facile synthesis of core-shell magnetic COF composite (Fe3O4@COF-OMe) to immobilize RML (Rhizomucor miehei lipase), to achieve its utilization in biodiesel production.Result: This strategy gives extrinsic magnetic property, and the magnetic COFs is much heavier and could disperse in water medium well, facilitating the attachment with the enzyme. The resultant biocomposite exhibited an excellent capacity of RML due to its high surface area and fast response to the external magnetic field, as well as good chemical stability. The core-shell magnetic COF-OMe structure not only achieved highly efficient immobilization and recovery processes but also maintained the activity of lipase to a great extent. RML@Fe3O4@COF-OMe performed well in practical applications, while free lipase did not. The biocomposite successfully achieved the production of biodiesel from Jatropha curcas Oil with a yield of about 70% in the optimized conditions. Conclusion: Magnetic COFs (Fe3O4@COF-OMe) for RML immobilization greatly improved catalytic performance in template reaction and biodiesel preparation. The magneticity makes it easily recovered and separated from the system. This first successful attempt of COFs-based immobilized enzyme broadened the prospect of biodiesel production by COFs with some inspiration.

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One-pot synthesis of trypsin-based magnetic metal–organic frameworks for highly efficient proteolysis

Abstract: A facile approach was developed for one-pot synthesis of trypsin-based magnetic ZIF-8, which could be applied as an immobilized enzyme microreactor for rapid digestion of proteins.

Cited by 18 publications

References 48 publications

Recent Advances in Emerging Metal– and Covalent–Organic Frameworks for Enzyme Encapsulation

Recent Advances in Emerging Metal– and Covalent–Organic Frameworks for Enzyme Encapsulation

Preparation and Characterization of Magnetic Metal–Organic Frameworks Functionalized by Ionic Liquid as Supports for Immobilization of Pancreatic Lipase

Magnetic COFs as Satisfied Support for Lipase Immobilization and Recovery to Effectively Achieve the Production of Biodiesel by Great Maintenance of Enzyme Activity

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