One-pot Synthesis of Quencher Labeled Hairpin DNA–CdTe QDs Conjugate for Target DNA and Deoxyribonuclease I Detection

Abstract

A black-hole quencher (BHQ-2) labeled DNA (Q-DNA) with a phosphorothioate backbone was covalently conjugated to the CdTe QDs during the QDs synthesis procedure. The hairpin structure of Q-DNA shortened the distance of the CdTe QDs and the BHQ-2 group, which resulted in fluorescence quenching of the QDs. The addition of target DNA or deoxyribonuclease I (DNase I) could move the BHQ-2 group away from the QDs. As a result, the fluorescence of the CdTe QDs recovered. This work provides a new way for target DNA and DNase I detection.

A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers

“…15 Nowadays, nanomaterials are widely used as nanoprobes, sensors or signal amplifier by many analysts. [16][17][18][19][20][21][22] Particularly, carbon nanotubes (CNTs) with new functional groups are ideal candidates for signal amplification due to their large surface area and the ability to integrate with biological elements. As a carrier, the CNTs were used to incorporate biomolecules (such as enzymes, antibodies, etc.)…”

Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission

“…These limitations are partially addressed by luminescence-based DNAse I assays that feature simplicity, sensitivity and ease of operation [21,22,23]. However, these methods often rely on the use of fluorophore-labelled DNA [24,25,26,27]. The high cost and synthetic challenges encountered in developing fluorophore-labelled DNA probes render label-free assays more convenient and cost-effective alternatives.…”

Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk

“…QDs 1-3 feature favorable biocompatibility, low toxicity and degradability, etc., they are of important application value in elds like biomolecular sensing, 4 environmental pollutant detection 5 and biomedicine, 6 so they have been extensively applied in practice. 7,8 Especially DNA molecules [9][10][11] have many advantages, including favorable thermal stability and an electronegative pentose-phosphate backbone which can easily bond with metal ions. [12][13][14] Moreover, they will not harm the environment aer degradation and can articially synthesize DNAs of specic sequences and form many aptamers of different sequences on QDs surfaces, which can contribute to preparation of multiple kinds of discriminatory analysis sensors of good selectivity.…”