One‐pot synthesis of highly fluorescent silicon nanoparticles for sensitive and selective detection of hemoglobin

Abstract

In this work, a simple, selective, and sensitive probe for hemoglobin based on the quenched fluorescence of silicon nanoparticles (SiNPs) was fabricated. The SiNPs were synthesized by a simple hydrothermal treatment from N‐[3‐(trimethoxysilyl)propyl]ethylenediamine and sodium citrate. The as‐prepared SiNPs exhibited good water‐solubility and high fluorescence with the quantum yield of 70%. The fluorescence of the SiNPs could be remarkably quenched by hemoglobin. A wide linear range was obtained from 50 nM to 4000 nM with a LOD of 40 nM. The quenching mechanism was investigated by UV‐Vis absorption spectrometry and time‐resolved fluorescence spectrometry.

Are Fluorescent Silicon Nanoparticles Formed in a One-Pot Aqueous Synthesis?

“…In 2013, a new aqueous synthetic approach based on one-pot synthesis of fluorescent Si NPs, using of (3-aminopropyl)­trimethoxysilane (APTMS) as the silicon source and trisodium citrate dihydrate (Na 3 Cit), was reported by Zhong et al This work has attracted a great deal of attention from the scientific community since 2013 and has accumulated 227 citations according to Google Scholar (June 6, 2019), 203 citations according to Scopus (May 18, 2019), and 191 citations according to SciFinder (June 6, 2019). Among all these papers, special attention should be paid to those that specifically mention use of the approach described in this paper for the basis of or jumping off point for their own work. − At first inspection, the proposed technique seems to have many advantages in comparison with most other approaches reported earlier. Of these advantages, one can emphasize in particular the following: (i) water is used as a solvent, (ii) short reaction times are sufficient (15 min), (iii) low temperatures are needed (160 °C), and (iv) inexpensive precursors work well.…”

One-pot synthesis of poly(vinylpyrrolidone)-encapsulated color-emitting silicon quantum dots for sensitive and selective detection of 2,4,6-trinitrophenol

“…3,44 In Fig. 2D, the XPS spectrum of O 1s showed various oxygen atoms; two atoms at 530.5 and 531.12 V correspond to Si-O, while the third one at 531.84 V to C-OH/C-O-C. 3,37,40,43 The XPS spectrum of Si 2p (Fig. 2E) revealed peaks at 101.4, 101.9, and 102.73 eV, which belonged to the Si-C, Si-N, and Si-O, respectively.…”

“…The peak at 781 cm À1 pertained to the secondary amine N-H wagging vibration bond. 3,37 Besides, the other peaks can be attributed to the stretching vibration of the CQO bond (1668 cm À1 ), 40 C-N bond (1286 cm À1 ), 42 Si-C bond (1019 cm À1 ), Si-O-Si bond (1121 cm À1 ) 40,43 and Si-O bond (930 cm À1 ), 28,40 respectively.…”