One‐pot synthesis of fluorescent nitrogen and sulfur–carbon quantum dots as a sensitive nanosensor for trimetazidine determination

Belal, Fathalla; Mabrouk, Mokhtar M.; Hammad, Sherin F.; Barseem, Aya; Ahmed, Hytham M.

doi:10.1002/bio.4083

Cited by 8 publications

(4 citation statements)

References 43 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescence spectroscopy and its various applications have been reviewed over the years in several excellent publications [25,[43][44][45][46][47][48][49]. Almost all proteins have a natural fluorescence because of the following amino acids (Phe, Tyr, and Trp) [50,51].…”

Section: Fluorometric Investigationmentioning

confidence: 99%

“…Therefore, the increase of rate constant for quenching with the temperature is the result. But, the rate constant of quenching for static quenching could get lower as the temperature raised [49,50,57,58]. At various temperature, Stern-Volmer plots were used for comprehending the mechanism for quenching by graphing F o /F in relation to Q as shown in figure 4 and K sv was calculated with change in temperature.…”

Section: Study Of Mechanism Of Quenchingmentioning

confidence: 99%

See 1 more Smart Citation

Probing the potential toxicity of trimetazidine by characterizing its interaction with human serum albumin

Barseem

Belal

Mabrouk

et al. 2023

Methods Appl. Fluoresc.

Self Cite

View full text Add to dashboard Cite

Since the small molecular drugs pharmacodynamics and pharmacokinetics could be affected by human serum albumin (HSA) transport, we used spectroscopic and molecular docking studies to investigate the interaction between HSA and the angina-prevention drug trimetazidine (TMZ). As shown by synchronous fluorescence spectroscopy, this interaction affects the microenvironment confirmation around tyrosine residues. The site-competitive experiments showed that TMZ had an affinity toward subdomain III A (site II) of HSA. The enthalpy and entropy changes (ΔH and ΔS), which were 37.75 and 0.197 K J mol-1, respectively, showed that the predominant intermolecular interactions stabilizing the TMZ-HSA combination are hydrophobic forces. According to FTIR research, the interaction between HSA and TMZ caused polypeptide carbonyl-hydrogen bonds to rearrange. Additionally, the HSA esterase enzyme activity was assessed, which revealed a decrease in activity with TMZ. Docking analysis confirmed the site-competitive experiments and thermodynamic results. Also, it showed that TMZ has strong affinity for binding to HSA and inserted into HSA subdomain III A (site II) with score of -12.65. This study demonstrated that TMZ interacted with HSA, and the structure and function of HSA were influenced by TMZ. This study could aid in understanding the pharmacokinetics of TMZ and provide basic data for safe use.

show abstract

Section: Fluorometric Investigationmentioning

confidence: 99%

Section: Study Of Mechanism Of Quenchingmentioning

confidence: 99%

Probing the potential toxicity of trimetazidine by characterizing its interaction with human serum albumin

Barseem

Belal

Mabrouk

et al. 2023

Methods Appl. Fluoresc.

Self Cite

View full text Add to dashboard Cite

show abstract

“…[ 31,32 ] Accompanied by its excellent optical properties, CDs present superior biocompatibility for bioanalytical and biomedical applications and as a consequence they have been applied to sensing, [ 33,34 ] imaging, [ 35,36 ] drug delivery, [ 37 ] and cancer theranostics. [ 38 ] Moreover, many pharmaceuticals recognition and quantification by fluorescent CDs have been reported, [ 39–44 ] validating the pharmaceutical analysis application of CDs.…”

Section: Introductionmentioning

confidence: 98%

Metal ion‐mediated carbon dot nanoprobe for fluorescent turn‐on sensing of N‐acetyl‐l‐cysteine

et al. 2022

View full text Add to dashboard Cite

In this work, carbon dots (CDs) was easily synthesized from aspartic acid through a pyrolysis method. Based on their favourable fluorescence properties, CDs were utilized to design a metal ion‐mediated fluorescent probe for N‐acetyl‐l‐cysteine (NAC) detection. The fluorescence intensity of CDs was firstly quenched by manganese ions (Mn2+) through static quenching effect and subsequently restored by NAC via the combination with Mn2+ due to the coordination effect. Therefore, the fluorescent turn‐on sensing of NAC was actuated based on the fluorescence quenching stimulated by Mn2+ and recovery induced by coordination. The fluorescence recovery efficiencies showed a proportional range to the concentration of NAC in the range 0.04–5 mmol L−1 and the detection limit was 0.03 mmol L−1. Furthermore, this metal ion‐mediated fluorescent nanoprobe was applied to human urine sample detection and the standard recovery rates were located in the range 97.62–102.34%. This was the first time that Mn2+ was used to construct a fluorescent nanoprobe for NAC. Compared with other heavy metal ions, Mn2+ with good biosecurity prevented the risk of application, which made the nanoprobe green and biopractical. The facile synthesis of CDs and novel metal ion‐mediated sensing mode made it a promising method for pharmaceutical analysis.

show abstract

“…Nitrogen (N) and sulfur (S) are commonly employed as doping elements in CQDs due to their comparable atomic sizes (the radius of C: 0.77, N: 0.75, S: 1.09), strong valence bonds, and greater electronegativities than carbon (electronegativity of C:2.55, N:3.4, S:2.58). Doping with N and S atoms generates extra electron pairs and defect sites, enhancing the number of active sites on the surface of CQDs, resulting in improved photoluminescent properties [29][30][31][32][33].…”

mentioning

confidence: 99%

Fluorimetric determination of Vonoprazan via quenching of nitrogen and sulfur co‐doped carbon quantum dots: A rapid and sustainable analytical approach

Barseem,

Elshahawy,

Elagamy

2024

Luminescence

Self Cite

View full text Add to dashboard Cite

In this study, an environmentally sustainable fluorimetric method for determination of Vonoprazan fumarate (VON) in dosage forms using nanoprobes consisting of nitrogen and sulfur co‐doped carbon quantum dots (N, S‐CQDs). The N, S‐CQDs were prepared through a microwave‐assisted method in 30 s. The resulting N, S‐CQDs were characterized using transmission electron microscopy (TEM), high‐resolution transmission electron microscopy (HRTEM), Fourier transform infrared spectroscopy (FTIR), and X‐ray photoelectron spectroscopy (XPS). They exhibit fluorescence emission at 460 nm after excitation at 385 nm with a high quantum yield (60%). The analytical approach for VON determination relies on the quenching effect exerted by VON on the native fluorescence intensity of CQDs. The quenching mechanism was investigated using Stern–Volmer plots. The proposed method demonstrates linearity across a concentration range 10–80 μM (4.6–36.8 μg/mL) with corresponding limits of detection and quantitation calculated as 2.17 μM (0.99 μg/mL) and 6.58 μM (3.02 μg/mL), respectively. The method has been effectively utilized for the determination of VON in the pharmaceutical samples. Statistical comparison with reported RP‐HPLC has been performed to verify the accuracy and precision of the developed method. The environmental sustainability of the developed method has been thoroughly examined through various greenness metrics.

show abstract

One‐pot synthesis of fluorescent nitrogen and sulfur–carbon quantum dots as a sensitive nanosensor for trimetazidine determination

Cited by 8 publications

References 43 publications

Probing the potential toxicity of trimetazidine by characterizing its interaction with human serum albumin

Probing the potential toxicity of trimetazidine by characterizing its interaction with human serum albumin

Metal ion‐mediated carbon dot nanoprobe for fluorescent turn‐on sensing of N‐acetyl‐l‐cysteine

Fluorimetric determination of Vonoprazan via quenching of nitrogen and sulfur co‐doped carbon quantum dots: A rapid and sustainable analytical approach

Contact Info

Product

Resources

About