One pot synthesis and anti-biofilm potential of copper nanoparticles (CuNPs) against clinical strains ofPseudomonas aeruginosa

Abstract

Pseudomonas aeruginosa, an opportunistic pathogen frequently associated with nosocomial infections, is emerging as a serious threat due to its resistance to broad spectrum antimicrobials. The biofilm mode of growth confers resistance to antibiotics and novel anti-biofilm agents are urgently needed. Nanoparticle based treatments and therapies have been of recent interest because of their versatile applications. This study investigates the anti-biofilm activity of copper nanoparticles (CuNPs) synthesized by the one pot method against P. aeruginosa. Standard physical techniques including UV-visible and Fourier transform infrared spectroscopy, X-ray diffraction and transmission electron microscopy were used to characterize the synthesized CuNPs. CuNP treatments at 100 ng ml(-1) resulted in a 94, 89 and 92% reduction in biofilm, cell surface hydrophobicity and exopolysaccharides respectively, without bactericidal activity. Evidence of biofilm inhibition was also seen with light and confocal microscope analysis. This study highlights the anti-biofilm potential of CuNPs, which could be utilized as coating agents on surgical devices and medical implants to manage biofilm associated infections.

Biofabrication of Zinc Oxide Nanoparticle fromOchradenus baccatusLeaves: Broad-Spectrum Antibiofilm Activity, Protein Binding Studies, andIn VivoToxicity and Stress Studies

“…Hsueh et al [54] have observed 92% reduced production of EPS by P. aeruginosa upon treatment with 100 ng/ml concentration of copper oxide nanoparticles. LewisOscar et al [55] also reported significant reduction of EPS produced by zinc oxide nanoparticle treated Bacillus subtilis strains.…”

Synthesis of hybrid copper-chitosan nanoparticles with antibacterial activity against cariogenic <i>Streptococcus mutans </i>

“…2) revealed a decrease in the intensities of the N-H amide/amine bending (~1,578 cm −1 ) and O-H/ N-H stretching bands (~3,303 cm −1 ) of chitosan structure as compared with the neat chitosan polymer 12) . In the case of neat CuNP, the peak around 2,100 cm −1 has been ascribed to the presence of atmospheric CO2 13) .…”

Antibiofilm activity of biogenic copper and zinc oxide nanoparticles-antimicrobials collegiate against multiple drug resistant bacteria: a nanoscale approach

“…Inspite of their bioactivity, the antibiofilm potential of CuNPs is rarely explored [17]. Only a few reports are published in the last year on, the use of copper-containing nanoparticles in the treatment of biofilms [9,18].…”