One-Pot Preparation of Imidazole-Ring-Modified Graphitic Carbon Nitride Nanozymes for Colorimetric Glucose Detection

Chen, Yuanyuan; Gao, Xueyou; Xue, Hang; Liu, Guohui; Zhou, Yue; Peng, Jian

doi:10.3390/bios12110930

Cited by 4 publications

(3 citation statements)

References 92 publications

(108 reference statements)

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“…As reported, carbonaceous materials such as g-C 3 N 4 , graphene oxide, carbon nanodots and graphene show peroxidase catalytic activity. [56][57][58][59][60][61][62] Therefore, the possible mechanism for the decomposition of H 2 O 2 in CNRG-CS is similar to that of horseradish peroxidase (HRP) which produces active enzyme intermediates, hydroxyl radicals and H + in its enzymatic catalytic reaction. The positive charges are then attached to the surface of CNRG-CS and induce positive field.…”

Section: Resultsmentioning

confidence: 99%

Electrolyte-gated field Effect Transistors Based on Cu Interdigitated Electrode for H₂O₂ and Glucose Sensing

Mohammadzadeh

Dehghani²,

Haghighi³

2023

J. Electrochem. Soc.

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A copper interdigitated electrode was patterned on a piece of printed circuit board. A layer of CNRG-CS composite prepared using graphitic carbon nitride, reduced graphene oxide and chitosan was immobilized on Cu interdigitated electrode as semiconductive channel. Then, the prepared CNRG-CS modified Cu interdigitated electrode was positioned into a home-made chamber to assemble an electrolyte-gated field effect transistor (EGFET)-based sensor for the non-enzymatic detection of H2O2. Also, glucose oxidase (GOx) was immobilized on CNRG-CS modified Cu interdigitated electrode to prepare CNRG-CS/GOx modified Cu interdigitated electrode and to assemble an EGFET-based biosensor for the enzymatic detection of glucose. Two linear dynamic ranges of 0.16-8.0 μM and 8.0-160 μM were obtained for H2O2 detection. Also, two linear dynamic ranges of 0.15-3.5 μM and 3.5-80 μM were obtained for glucose detection. The detection limits (S/N=3) for H2O2 and glucose were 0.12 and 0.14 μM, respectively. The relative standard deviations for repetitive measurements (n=3) of H2O2 (3.0 μM) and glucose (3.0 μM) were 1.8 and 2.5%, respectively. The fabrication reproducibility for five EGFET-based sensors and biosensors prepared and used in different days were 7.4 and 8.2%, respectively.

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Section: Resultsmentioning

confidence: 99%

Electrolyte-gated field Effect Transistors Based on Cu Interdigitated Electrode for H₂O₂ and Glucose Sensing

Mohammadzadeh

Dehghani²,

Haghighi³

2023

J. Electrochem. Soc.

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“…The as‐prepared C 3 N 4 ‐Im‐Cu nanozymes showed high sensitivity as a colorimetric biosensor of glucose with a LOD value of 10 n m . [ 208 ] Pepsin templated Cu NCs (Cu 23 @pepsin) were synthesized with optimal POD‐like activity pH at 7. Using OPD as chromogenic substrate, the Cu 23 @pepsin NCs were well applied for the fluorometric sensing of glucose with a LOD value of 7.56 µ m under neutral condition.…”

Section: Application Of Nanozymes Without Ph Limitationmentioning

confidence: 99%

Breaking the pH Limitation of Nanozymes: Mechanisms, Methods, and Applications

Feng,

Wang,

Wang

et al. 2024

Advanced Materials

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Although nanozymes have drawn a great attention over the past decade, the activities of peroxidase‐like, oxidase‐like and catalase‐like nanozymes are often pH dependent with elusive mechanism, which largely restricts their application. Therefore, a systematical discussion on the pH related catalytic mechanisms of nanozymes together with the methods to overcome this limitation is in need. In this review, various nanozymes exhibiting pH dependent catalytic activities are collected and the root causes for their pH dependence are comprehensively analyzed. Subsequently, regulatory concepts including catalytic environment reconstruction and direct catalytic activity improvement to break this pH restriction are summarized. Moreover, applications of pH independent nanozymes in sensing, disease therapy and pollutant degradation are overviewed. Finally, current challenges and future opportunities on the development of pH independent nanozymes are suggested. It is anticipated that this review will promote the further design of pH independent nanozymes and broaden their application range with higher efficiency.This article is protected by copyright. All rights reserved

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“…There is a wide collection of nanomaterials that reproduce the functions of natural enzymes [ 14 , 15 , 16 , 17 , 18 , 19 ]. The most studied aspect is the peroxidase-like activity of nanozymes, which is the catalytic conversion of hydrogen peroxide to water [ 20 , 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Fe-Doped Peroxidase Mimetic Nanozymes from Natural Hemoglobin for Colorimetric Biosensing and In Vitro Anticancer Effects

Mohammadpour,

Askari,

Shokati

et al. 2023

Biosensors

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: Despite their efficiency and specificity, the instability of natural enzymes in harsh conditions has inspired researchers to replace them with nanomaterials. In the present study, extracted hemoglobin from blood biowastes was hydrothermally converted to catalytically active carbon nanoparticles (BDNPs). Their application as nanozymes for the colorimetric biosensing of H2O2 and glucose and selective cancer cell-killing ability was demonstrated. Particles that were prepared at 100 °C (BDNP-100) showed the highest peroxidase mimetic activity, with Michaelis–Menten constants (Km) of 11.8 mM and 0.121 mM and maximum reaction rates (Vmax) of 8.56 × 10−8 mol L−1 s−1 and 0.538 × 10−8 mol L−1 s−1, for H2O2 and TMB, respectively. The cascade catalytic reactions, catalyzed by glucose oxidase and BDNP-100, served as the basis for the sensitive and selective colorimetric glucose determination. A linear range of 50–700 µM, a response time of 4 min, a limit of detection (3σ/N) of 40 µM, and a limit of quantification (10σ/N) of 134 µM was achieved. In addition, the reactive oxygen species (ROS)-generating ability of BDNP-100 was employed for evaluating its potential in cancer therapy. Human breast cancer cells (MCF-7), in the forms of monolayer cell cultures and 3D spheroids, were studied by MTT, apoptosis, and ROS assays. The in vitro cellular experiments showed dose-dependent cytotoxicity of BDNP-100 toward MCF-7 cells in the presence of 50 µM of exogenous H2O2. However, no obvious damage was induced to normal cells in the same experimental conditions, verifying the selective cancer cell-killing ability of BDNP-100.

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One-Pot Preparation of Imidazole-Ring-Modified Graphitic Carbon Nitride Nanozymes for Colorimetric Glucose Detection

Cited by 4 publications

References 92 publications

Electrolyte-gated field Effect Transistors Based on Cu Interdigitated Electrode for H₂O₂ and Glucose Sensing

Electrolyte-gated field Effect Transistors Based on Cu Interdigitated Electrode for H₂O₂ and Glucose Sensing

Breaking the pH Limitation of Nanozymes: Mechanisms, Methods, and Applications

Synthesis of Fe-Doped Peroxidase Mimetic Nanozymes from Natural Hemoglobin for Colorimetric Biosensing and In Vitro Anticancer Effects

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One-Pot Preparation of Imidazole-Ring-Modified Graphitic Carbon Nitride Nanozymes for Colorimetric Glucose Detection

Cited by 4 publications

References 92 publications

Electrolyte-gated field Effect Transistors Based on Cu Interdigitated Electrode for H2O2 and Glucose Sensing

Electrolyte-gated field Effect Transistors Based on Cu Interdigitated Electrode for H2O2 and Glucose Sensing

Breaking the pH Limitation of Nanozymes: Mechanisms, Methods, and Applications

Synthesis of Fe-Doped Peroxidase Mimetic Nanozymes from Natural Hemoglobin for Colorimetric Biosensing and In Vitro Anticancer Effects

Contact Info

Product

Resources

About

Electrolyte-gated field Effect Transistors Based on Cu Interdigitated Electrode for H₂O₂ and Glucose Sensing

Electrolyte-gated field Effect Transistors Based on Cu Interdigitated Electrode for H₂O₂ and Glucose Sensing