One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase

Sriariyanun, Malinee; Kitiborwornkul, Nichaphat; Tantayotai, Prapakorn; Rattanaporn, Kittipong; Show, Pau Loke

doi:10.3390/bioengineering9010017

Cited by 37 publications

(10 citation statements)

References 40 publications

(62 reference statements)

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“…This enzymatic inhibition might decrease the rate of saccharification and microbial fermentation. Second- and third-generation ILs (e.g., cholinium salts) are more sustainable and cause less toxicity, maintaining the integrity of the enzymes [ 8 , 48 , 49 , 50 ]. The pursuit of IL bio-derived and enzyme-tolerant cocktails is increasing and yielding excellent results [ 44 ]; then, the saccharification and fermentation can be combined, eliminating the separation of hydrolysates prior to the fermentation [ 47 , 51 ].…”

Section: One-pot Integration Of the Saccharification And Fermentation...mentioning

confidence: 99%

Challenges in Using Ionic Liquids for Cellulosic Ethanol Production

et al. 2023

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The growing need to expand the use of renewable energy sources in a sustainable manner, providing greater energy supply security and reducing the environmental impacts associated with fossil fuels, finds in the agricultural by-product bioethanol an economically viable alternative with significant expansion potential. In this regard, a dramatic boost in the efficiency of processes already in place is required, reducing costs, industrial waste, and our carbon footprint. Biofuels are one of the most promising alternatives to massively produce energy sustainably in a short-term period. Lignocellulosic biomass (LCB) is highly recalcitrant, and an effective pretreatment strategy should also minimize carbohydrate degradation by diminishing enzyme inhibitors and other products that are toxic to fermenting microorganisms. Ionic liquids (ILs) have been playing an important role in achieving cleaner processes as a result of their excellent physicochemical properties and outstanding performance in the dissolution and fractionation of lignocellulose. This review provides an analysis of recent advances in the production process of biofuels from LCB using ILs as pretreatment and highlighting techniques for optimizing and reducing process costs that should help to develop robust LCB conversion processes.

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Section: One-pot Integration Of the Saccharification And Fermentation...mentioning

confidence: 99%

Challenges in Using Ionic Liquids for Cellulosic Ethanol Production

et al. 2023

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“…The hydrolysis reaction was performed using 1 g of biomass and a hydrolysis buffer solution consisting of 40 ml of 50 mM citrate buffer, 400 µl of 2M sodium azide, and 200 µl of CTec2. The reaction was conducted at 50 o C and 150 rpm for a duration of 72 hours [19,20].…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production

Panyarachun,

Panakkal,

Tawai

et al. 2023

E3S Web Conf.

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Thailand as an agricultural country faces significant challenges in managing the abundant biomass waste generated from agricultural activities. Conventional disposal methods such as incineration contribute to pollution and limited availability of landfill space. To mitigate these issues valorization of this biomass waste has been a solution. This study focuses on the utilization of Napier grass as a renewable energy source. In this experiment, the Napier grass samples were pretreated using oxalic acid with temperature variations (50 – 100 °C), time (30 180 min), and oxalic acid concentration (2 10%w/v) to determine the limit of these three factors for optimization studies. The utilization of Box-Behnken Design (BBD) within Response Surface Methodology (RSM) enabled the determination of optimal pretreatment conditions and the exploration of the correlation between pretreatment factors and reducing sugar content. The model predicted pretreatment with an oxalic acid concentration of 6% w/v, pretreated at 100 °C for 105 min as the optimal pretreatment condition to produce a maximum reducing sugar concentration of 10.65 mg/ml. Therefore, the sample was pretreated at optimum conditions and the results revealed the amount of reducing sugar obtained was 10.67 mg/ml, which differed from the predicted value with an error of 0.22%. Thus, this study provides insight for future researchers on the optimum condition that can be applied for pretreating biomass with oxalic acid to maximize the sugar yield.

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“…Further, besides the use of inexpensive ILs with a considerable amount of water, biomass loading is utilized, being an important and economically justified parameter for the process. The acceptable rate of biomass loading is 10% in the literature 24 – 26 . Thus, the application of a high biomass load that gives rise to greater possibilities for the industrial pretreatment process should be taken into consideration 27 .…”

Section: Introductionmentioning

confidence: 99%

Investigation of a robust pretreatment technique based on ultrasound-assisted, cost-effective ionic liquid for enhancing saccharification and bioethanol production from wheat straw

Ziaei-Rad

Pazouki

Fooladi

et al. 2023

Sci Rep

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Application of cost-effective pretreatment of wheat straw is an important stage for massive bioethanol production. A new approach is aimed to enhance the pretreatment of wheat straw by using low-cost ionic liquid [TEA][HSO4] coupled with ultrasound irradiation. The pretreatment was conducted both at room temperature and at 130 °C with a high biomass loading rate of 20% and 20% wt water assisted by ultrasound at 100 W-24 kHz for 15 and 30 min. Wheat straw pretreated at 130 °C for 15 and 30 min had high delignification rates of 67.8% and 74.9%, respectively, and hemicellulose removal rates of 47.0% and 52.2%. Moreover, this pretreatment resulted in producing total reducing sugars of 24.5 and 32.1 mg/mL in enzymatic saccharification, respectively, which corresponds to saccharification yields of 67.7% and 79.8% with commercial cellulase enzyme CelluMax for 72 h. The ethanol generation rates of 38.9 and 42.0 g/L were attained for pretreated samples for 15 and 30 min, equivalent to the yields of 76.1% and 82.2% of the maximum theoretical yield following 48 h of fermentation. This demonstration provided a cheap and promising pretreatment technology in terms of efficiency and shortening the pretreatment time based on applying low-cost ionic liquid and efficient ultrasound pretreatment techniques, which facilitated the feasibility of this approach and could further develop the future of biorefinery.

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One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase

Cited by 37 publications

References 40 publications

Challenges in Using Ionic Liquids for Cellulosic Ethanol Production

Challenges in Using Ionic Liquids for Cellulosic Ethanol Production

Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production

Investigation of a robust pretreatment technique based on ultrasound-assisted, cost-effective ionic liquid for enhancing saccharification and bioethanol production from wheat straw

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