One-Pot Hydrothermal Preparation of Hydroxyapatite/Zinc Oxide Nanorod Nanocomposites and Their Cytotoxicity Evaluation against MG-63 Osteoblast-like Cells

Abstract

In the present study, HAp-ZnO nanorod nanocomposites were successfully prepared using a customized hydrothermal reactor and studied for their compatibility against MG-63 osteoblast-like cells. The crystallinity, morphology, presence of chemical elements, and surface area properties were studied by XRD (X-ray diffraction), FE-SEM (field emission scanning electron microscopy), TEM (transmission electron microscopy), EDS (energy dispersive spectrum) and N2 adsorption/desorption isotherm techniques, respectively. Further, the mechanical strength and thermal analysis were carried out using the nanoindentation method and thermogravimetric/differential scanning calorimeter (TG/DSC) methods, respectively. Moreover, in vitro biocompatibility studies for the prepared samples were carried out against human osteosarcoma cell lines (MG-63). The crystalline nature of the samples without any impurity phases was notified from XRD results. The formation of composites with the morphology of nanorods and the presence of desired elements in the intended ratio were verified using FE-SEM and EDS spectra, respectively. The TG/DSC results revealed the improved thermal stability of the HAp matrix, promoted by the reinforcement of the ZnO nanorods. The nanoindentation study ensured a significant enhancement in the mechanical stability of the prepared composite material. Finally, it demonstrated that the HAp matrix’s mechanical strength and thermal stability were improved by the reinforcement of ZnO, and the cytotoxicity evaluation affirmed the biocompatible nature of the biomimetic hydroxyapatite in the composite.

Bioactive glass doped with zinc-based metal–organic frameworks (Zn/MOF) nanoparticles for antibiotic delivery applications

“…In vitro cytocompatibility studies were investigated to predict the biological reactions of materials when applied in vivo [51]. In vitro cell viability (SRB assay) studies were performed on human osteoblast-like cell cultures (MG-63), which have the same physiology and adhesiveness as human osteoblast cells [52]. The cell viability percent against different concentrations (100 and 300 μg/ml) of BG and BG/Zn MOF samples are illustrated in Fig.…”

Screening of low‐toxic zinc oxide nanomaterials and study the apoptosis mechanism of NSC‐34 cells

“…[7] Previous research has shown that this approach allows for control over morphology, size, and crystal structure. [8][9][10][11] ZnO-NMts can be classified into different dimensions based on their morphology. Zero-dimensional nanomaterials, like ZnO NPs, have spherical or quasi-spherical shapes and are widely used in biomedical research.…”

Synthesis and Characterization of Nano-Hydroxyapatite Obtained from Eggshell via the Hydrothermal Process and the Precipitation Method

“…HA is the main mineral component of hard tissues such as bones and teeth, and it exhibits excellent bioactivity, biocompatibility, and osteoconductivity [ 2 ]. HA is non-toxic and does not produce an inflammatory reaction in the body, so it is widely used in bone-filling materials, tissue engineering scaffolds, and drug carriers [ 3 ].…”