One Pot Green Synthesis of Doxorubicin and Curcumin Loaded Magnetic Nanoparticles and Cytotoxicity Studies

Abstract

Background: Green synthesis, an alternative method for synthesizing nanoparticles, is cheaper, environmentally friendly, and does not produce toxic effects. Doxorubicin is a chemotherapeutic agent used in lung cancer. Curcumin is a bioactive compound with properties such as an anticancer obtained from Curcuma longa. Objective: This study’s objective was to develop Doxorubicin and Curcumin loaded magnetic nanoparticles that could be synthesized by green tea leaves and investigate cytotoxic effects against the A549-luc-C8, non-small cell lung cancer line. Methods: Magnetic nanoparticles were synthesized with the green synthesis method. Furthermore, Doxorubicin and Curcumin were encapsulated into magnetic nanoparticles with the one-pot method and obtained magnetic nanoparticles characterized using FTIR, SEM/EDX, XRD, and UV-VIS spectrophotometric techniques. After that, The drug release test was performed by dialysis using pH 7.4 phosphate-buffered saline at 37 °C. MTT assay was performed to test the cytotoxicity effect in the A549-luc-C8 cell line. Results: FTIR analysis verified the magnetic structure and drug loading. SEM images of magnetic nanoparticle releaved that they had a size of about 50-60 nm in a mono-disperse manner. Drug release after 24 h was found as 5.8% for doxorubicin and 3.4% for curcumin, showing controlled release. Conclusion: Results showed that the prepared magnetic nanoparticles were thought to had synergistic antitumor activity for A549-lucC8.

One‐Pot Synthesis of Doxorubicin‐Bioactive Glass‐Ceramic Hybrid Nanoparticles through a Bio‐Inspired Route for Anti‐Cancer Therapy

“…From studies invoving one-pot synthesis of doxorubicinsilica [22][23][24][25][26][27] and other classes of nanoparticles, [28][29][30] it can be predicted that functional groups of the drug can react with precursors of bioactive glass. Precursors of bioactive glass like tetraethoxysilanes on getting hydrolyzed to subunits of silicon oxides can react with hydroxyl groups of doxorubicin thus allowing incorporation of the same during different stages of the growth of the nanoparticles.…”

Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirwayTM Microtissue

“…Nevertheless, the scientific literature presents few reports regarding the fabrication of magnetic iron oxides nanoparticles through green synthesis approach with anticancer potential against lung cancer [ 21 , 22 , 23 ]. No study has previously reported the use of basil as plant material for MNPs’ biosynthesis, whereas only one study reports the MNPs’ biosynthesis using green tea extract, as well as the cytotoxic effect of this MNPs against A549-luc-C8 non-small lung cancer line [ 24 ]. However, in that study [ 22 ], the authors did not investigate the cytotoxic effect of naked MNPs (obtained as such from green synthesis), they loaded the MNPs obtained with doxorubicin and curcumin and afterwards, the cytotoxic effect of the nanoformulation against NSCLC line was investigated.…”