One Heat Shock Transcription Factor Confers High Thermal Tolerance in Clematis Plants

Wang, Rui; Mao, Chanjuan; Jiang, Changhua; Zhang, Long; Peng, Siyuan; Zhang, Yi; Feng, Shucheng; Ming, Feng

doi:10.3390/ijms22062900

Cited by 8 publications

(10 citation statements)

References 49 publications

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“…6 b and 10 ), suggesting that HSFA2 may play an important role in the heat response of C. crassifolia . CvHSF30-2 was a transcription factor induced by heat stress in Clematis vitalba , which improved the heat tolerance of C. vitalba by increasing the expression of HSPs [ 73 ]. Upregulation of HSF30 ( c194517_g1 ) was also observed in C. lanuginosa and C. crassifolia (Fig.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

Qian

et al. 2022

BMC Plant Biol

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Background Clematis species are attractive ornamental plants with a variety of flower colors and patterns. Heat stress is one of the main factors restricting the growth, development, and ornamental value of Clematis. Clematis lanuginosa and Clematis crassifolia are large-flowered and evergreen Clematis species, respectively, that show different tolerance to heat stress. We compared and analyzed the transcriptome of C. lanuginose and C. crassifolia under heat stress to determine the regulatory mechanism(s) of resistance. Results A total of 1720 and 6178 differentially expressed genes were identified from C. lanuginose and C. crassifolia, respectively. The photosynthesis and oxidation–reduction processes of C. crassifolia were more sensitive than C. lanuginose under heat stress. Glycine/serine/threonine metabolism, glyoxylic metabolism, and thiamine metabolism were important pathways in response to heat stress in C. lanuginose, and flavonoid biosynthesis, phenylalanine metabolism, and arginine/proline metabolism were the key pathways in C. crassifolia. Six sHSPs (c176964_g1, c200771_g1, c204924_g1, c199407_g2, c201522_g2, c192936_g1), POD1 (c200317_g1), POD3 (c210145_g2), DREB2 (c182557_g1), and HSFA2 (c206233_g2) may be key genes in the response to heat stress in C. lanuginose and C. crassifolia. Conclusions We compared important metabolic pathways and differentially expressed genes in response to heat stress between C. lanuginose and C. crassifolia. The results increase our understanding of the response mechanism and candidate genes of Clematis under heat stress. These data may contribute to the development of new Clematis varieties with greater heat tolerance.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

Qian

et al. 2022

BMC Plant Biol

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“…Diaminobenzidine (DAB) and nitrotetrazolium blue (NBT) staining: take control plants or leaves of irradiated plants as samples, add DAB or NBT staining solution (both liquids were not over the samples), heat the water bath at 100 °C for 5 min, leave the staining solution for 16 h in a place protected from light, pour out the staining solution, wash the samples with distilled water 2~3 times, add anhydrous ethanol, heat the water bath for 5 min, pour out the ethanol, repeat until decolorization, add 40% volume fraction of glycerol solution, leave the solution in a place protected from light for 2 h, pour out the glycerol solution, and observe [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Mutagenic Effect of 60Co γ-Irradiation on Rosa multiflora ‘Libellula’ and the Mechanism Underlying the Associated Leaf Changes

Xia

Liu

et al. 2022

Plants

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Gamma (γ)-irradiation can induce changes in plant morphology, cellular physiological activities, and genetic material. To date, there has been limited research on the molecular basis of leaf morphological abnormalities and physiological changes in irradiated rose plants. In this study, Rosa multiflora ‘Libellula’ plants were treated with 60Co γ-rays. The irradiation resulted in the distortion of blade morphology. Additionally, the leaf chlorophyll content decreased, whereas the accumulation of reactive oxygen species increased. The differentially expressed genes between the control and 2–3 plants irradiated with 50 Gy were analyzed by RNA-seq technology, which revealed genes related to chlorophyll metabolism were differentially expressed. The expression levels of genes related to the regulation of antioxidant enzyme synthesis were downregulated. An RNA-seq analysis also identified the differentially expressed regulatory genes involved in leaf morphology development. Four genes (RcYABBY1, RcARF18, RcARF9, and RcWOX8) were selected, and their expression patterns in different leaf development stages and in various plant organs were analyzed. Furthermore, virus-induced gene silencing technology was used to verify that RcYABBY1 is involved in the morphogenesis of R. multiflora ‘Libellula’ leaves. The results of this study are useful for clarifying the molecular, physiological, and morphological changes in irradiated rose plants.

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“…Maize (Zea mays) mitochondrial sHsps showed protection against NADH and improved mitochondrial electron b transport during salt stress [8]. The accumulation of SlHSP21.5A can increase the tolerance of rice (Oryza sativa) to salt stress [9]; overexpression of CaHSP25.9 can enhance pepper (Piper nigrum) tolerance to heat, salt and drought stress; CaHSP16.4 is related to pepper (Piper nigrum) heat and drought tolerance [10,11]; overexpression of KSlHSP21.5A can enhance tomato (Solanum lycopersicum) tolerance to heat stress [12]; the expression of CvHSF30-2 can improve the heat resistance of clematis (Clematis tibetana) [13]. More and more studies have shown that there is a strong correlation between the accumulation of sHsps and the stress tolerance of plants.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Drought Resistance Analysis of Soybean GmHsps_p23-like Gene

Zhang¹,

Li²,

Hu³

et al. 2022

Phyton

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Soybean (Glycine max (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean GmHsps_p23-like gene was successfully cloned by RT-PCR, the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. Agrobacterium-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance physiological and biochemical index detection were performed on the T 2 generation positive transgenic soybean plants identified by PCR and Southern hybridization. The results showed that the overexpression vector plant GmHsps_p23-like gene expression increased. After rehydration, the transgenic overexpression plants returned to normal growth, and the damage to the plants was low. After drought stress, the SOD and POD activities and the PRO content of the transgenic overexpression plants increased, while the MDA content decreased. The reverse was true for soybean plants with genetically modified editing vectors. The drought resistance of the overexpressed soybeans under drought stress was higher than that of the control group, and had a stronger drought resistance. It showed that the expression of soybean GmHsps_p23-like gene can improve the drought resistance of soybean. The cloning and functional verification of soybean GmHsps_p23-like gene had not been reported yet. This is the first time that PCR technology has been used to amplify the soybean GmHsps_p23-like gene and construct an expression vector for this gene. This research has laid the foundation for transgenic technology to improve plant drought resistance and cultivate new drought-resistant transgenic soybean varieties.

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One Heat Shock Transcription Factor Confers High Thermal Tolerance in Clematis Plants

Cited by 8 publications

References 49 publications

Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

Mutagenic Effect of 60Co γ-Irradiation on Rosa multiflora ‘Libellula’ and the Mechanism Underlying the Associated Leaf Changes

Cloning and Drought Resistance Analysis of Soybean GmHsps_p23-like Gene

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