One global disseminated 193 kb high-risk hybrid plasmid harboring tet(X4), mcr or blaNDM threatening public health

Li, Min; Zhang, Haoran; Zhang, Wengang; Cao, Ying; Sun, Bingqing; Qin, Jingui; Liu, Haodong; Guo, WenNan; Chen, Cheng; Zhou, Nan; Lv, Chao; Guo, Chaoyi; Guo, Xiaokui; Shang, Jun; Huang, Shixin; Zhu, Yongzhang

doi:10.1016/j.scitotenv.2023.162807

Cited by 8 publications

(3 citation statements)

References 42 publications

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“…The conserved genetic context of the strains identified in our study was consistent with that of previous studies [ 22 , 23 ]. However, some studies have reported that tet (X4)-carrying plasmids contain numerous mobile elements, such as IS 26 , IS 3000 , and IS 1 , along with other resistance genes, including aadA22 , bla TEM-1B , sulI , and dfrA12 [ 24 , 25 ]. To the best of our knowledge, only a limited number of studies have explored structures containing tet (A) and tet (R) [ 26 ] isolated from animal food and environmental samples.…”

Section: Discussionmentioning

confidence: 99%

Emergence of plasmid-mediated tigecycline resistance tet(X4) gene in Enterobacterales isolated from wild animals in captivity

Lei,

Xiong,

Yan

et al. 2024

Science in One Health

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Section: Discussionmentioning

confidence: 99%

Emergence of plasmid-mediated tigecycline resistance tet(X4) gene in Enterobacterales isolated from wild animals in captivity

Lei,

Xiong,

Yan

et al. 2024

Science in One Health

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“…Annually, more than 700,000 people succumb to infections caused by antimicrobial-resistant microbes. Experts predicted that by 2050, this number might reach 10 million with an estimated cost of $100 trillion 1 , 2 .…”

Section: Introductionmentioning

confidence: 99%

Carbapenem-resistant Escherichia coli exhibit diverse spatiotemporal epidemiological characteristics across the globe

Huang,

Lv,

et al. 2024

Commun Biol

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Carbapenem-resistant Escherichia coli (CREC) poses a severe global public health risk. This study reveals the worldwide geographic spreading patterns and spatiotemporal distribution characteristics of resistance genes in 7918 CREC isolates belonging to 497 sequence types (ST) and originating from 75 countries. In the last decade, there has been a transition in the prevailing STs from highly virulent ST131 and ST38 to higher antibiotic-resistant ST410 and ST167. The rise of multi-drug resistant strains of CREC carrying plasmids with extended-spectrum beta-lactamase (ESBL) resistance genes could be attributed to three important instances of host-switching events. The spread of CREC was associated with the changing trends in blaNDM-5, blaKPC-2, and blaOXA-48, as well as the plasmids IncFI, IncFII, and IncI. There were intercontinental geographic transfers of major CREC strains. Various crucial transmission hubs and patterns have been identified for ST131 in the United Kingdom, Italy, the United States, and China, ST167 in India, France, Egypt, and the United States, and ST410 in Thailand, Israel, the United Kingdom, France, and the United States. This work is valuable in managing CREC infections and preventing CREC occurrence and transmission inside healthcare settings and among diverse hosts.

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“…ntimicrobial resistance (AMR) is a significant global public health threat, with over 700,000 65 people dying annually from infections caused by AMR. Experts predict that 2050 annual deaths 66 due to AMR will reach 10 million, costing $100 trillion (1,2). The prevalence of extended-spectrum β-lactamases (ESBLs) significantly contributes to the current antibiotic resistance crisis.…”

Section: Introductionmentioning

confidence: 99%

Role of Phage–Antibiotic Combinations in Reducing ESBL-Producing and Carbapenem-ResistantEscherichia coli

Shamsuzzaman,

Kim,

Kim

2024

Preprint

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The emergence of extended-spectrumβ-lactamase (ESBL)-producingE. coliand carbapenem-resistantE. coli(CREC) poses a significant global health concern. Here, we isolated and characterized two novel phages and studied their effectiveness with antibiotics against ESBL-producingE. coliand CREC. The isolated phages, EC.W1-9 and EC.W15-4, belonged to thePodoviridaeandMyoviridaefamilies, respectively. They are safe for bacterial control as they do not contain integrase or toxin-coding genes. The phage combination considerably enhanced lytic ability, effectively lysing 61.7% of the 60E. coliisolates, compared to lysis in the 41.6% –55% range by individual phages. Phages EC.W1-9 and EC.W15-4 combined demonstrated 100% susceptibility against differentE. colisequence types, including ST73, ST648, ST2311, ST405, ST7962, ST131, ST13003, and ST167. Additionally, studies showed synergy between antibiotics and phage combinations against ESBL-producingE.coli, with susceptibility of 73.3% and 54% for CREC. The combined treatment of isolated phages and antibiotics significantly increased survival rates in BALB/c mice exposed to various ST types of ESBL-producingE. coliand CREC, including ST131, ST648, and ST410. Survival rates against KBN7288 (ST131) increased by approximately 75% and 50% compared to individual phages EC.W1-9 and EC.W15-4, respectively. When phages and antibiotics were combined, survival rates againstE. coliisolates KBN5617 (ST410), KBN6241 (ST410), and KBN4004 (ST648) ranged from 75% – 100%. Finally, this study highlights the importance of phage and phage-antibiotic combinations to prepare phages for killing different ST types of ESBL-producingE. coliand CREC isolates.IMPORTANCEWhen combined with antibiotics, phage therapy shows promise in fighting multidrug-resistant bacteria. However, antagonism between phages and antibiotics has been reported. This research isolates and characterizes two novel phages, EC.W1-9 and EC.W15-4, from thePodoviridaeandMyoviridaefamilies, respectively, and evaluates their effectiveness against ESBL-producingE. coliand CREC. These phages, lacking integrase or toxin-coding genes, showed significant promise in bacterial control. Combined phage treatment lysed 61.7% ofE.coliisolates, outperforming individual phages. The phage combination showed 100% susceptibility against differentE. colisequence types. Additionally, the synergy between phages and antibiotics increased susceptibility rates to 73.3% for ESBL-producingE. coliand 54% for CREC. In BALB/c mice, combined treatments significantly improved survival rates against variousE. coliisolates. Finally. this study emphasizes the potential of phage and phage-antibiotic combinations in targeting various ST types of ESBL-producingE. coliand CREC.

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One global disseminated 193 kb high-risk hybrid plasmid harboring tet(X4), mcr or blaNDM threatening public health

Cited by 8 publications

References 42 publications

Emergence of plasmid-mediated tigecycline resistance tet(X4) gene in Enterobacterales isolated from wild animals in captivity

Emergence of plasmid-mediated tigecycline resistance tet(X4) gene in Enterobacterales isolated from wild animals in captivity

Carbapenem-resistant Escherichia coli exhibit diverse spatiotemporal epidemiological characteristics across the globe

Role of Phage–Antibiotic Combinations in Reducing ESBL-Producing and Carbapenem-ResistantEscherichia coli

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