One-factor-at-a-time (OFAT) optimization of β-galactosidase production from Aspergillus flavus gr. NFCCI-2728 in submerged fermentation

Abstract

Introduction: Submerged fermentations (SmF) for enzymes production are typically applied with a substrate, that is either dissolved or remains suspended in an aqueous medium. For majority of the massive scale enzyme production SmF technology is most popular because of higher observance and simple handling (Pandey et al., 2010). Optimization of fermentation conditions, significantly physical and chemical parameters, is vital within the development of fermentation processes because of their impact on the economy and usefulness of the method. The growth and enzyme production of the organism are powerfully influenced by medium composition therefore improvement of media elements and cultural parameters is that the primary task during a process (Pandey et al., 2003). To satisfy the growing demands within the industry it's necessary to enhance the performance of the system and therefore increase the yield while not increasing the value of production. Enzyme prices are often reduced by finding optimum conditions for their production (Ponce-Noyola and de la Tom, 2001). β-galactosidase belongs to the cluster of saccharides converting enzymes within the family of hydrolases. It's unremarkably referred to as lactase. They're widespread distributed in various biological systems, e.g. microorganisms, plants and animal tissues. Compared to animal and plant sources

β-Galactosidase-Producing Isolates in Mucoromycota: Screening, Enzyme Production, and Applications for Functional Oligosaccharide Synthesis

“…For instance, optimized yields were reached for Aspergillus tubingensis (15,936 U/g substrate) [ 18 ], Penicillium canescens (5292 U/g substrate) [ 39 ], and Trichoderma sp. (2.67 U/g substrate) [ 31 ] in SSF systems, and for Trichoderma reesei (25.43 U/mL) [ 40 ], Aspergillus niger (from 0.279 to 0.764 U/mL) [ 41 ] and Aspergillus flavus (843.75 U/mg protein) [ 42 ] in SmF systems.…”