One-enzyme catalyzed simultaneous plant cell disruption and conversion of released glycoside to aglycone combined with in situ product separation as green one-pot production of genipin from gardenia fruit

Winotapun, Weerapath; Opanasopit, Praneet; Ngawhirunpat, Tanasait; Rojanarata, Theerasak

doi:10.1016/j.enzmictec.2013.05.001

Cited by 28 publications

(12 citation statements)

References 31 publications

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“…In the selected pigments, PSPC is an acylated anthocyanidin and SY belongs to chalcone group with the same type C6-C3-C6 backbone structure as anthocyanidin, whereas GB is a rare natural blue pigment with iridoid base structure. The structure of pigments [23][24][25] RB and is slightly better than commercial Degussa P25 TiO 2 on visible light activity. By contrast, only MTiO 2 /PSPC shows 29.8% of photodegradation yields after 4 h visible-light irradiation.…”

Section: Photocatalytic Activity Of Mesoporousmentioning

confidence: 87%

Visible-Light Degradation of Dyes and Phenols over Mesoporous Titania Prepared by Using Anthocyanin from Red Radish as Template

Yan

Gong

Chen

et al. 2014

International Journal of Photoenergy

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Heterogeneous photocatalysis is able to operate effectively to eliminate organic compounds from wastewater in the presence of semiconductor photocatalyst and a light source. Although photosensitization of titania by organic dyes is one of the conventional ways for visible-light utilization of titania, previous studies have not yet addressed the use of natural food coloring agents as templates in the synthesis of mesostructured materials, let alone the simultaneous achievement of highly crystalline mesoscopic framework and visible-light photocatalytic activity. In this work, anthocyanin, a natural pigment from red radish was directly used as template in synthesis of highly crystalline mesoporous titania. The synthesized mesoporous titania samples were characterized by a combination of various physicochemical techniques, such as XRD, SEM, HRTEM, nitrogen adsorption/desorption, and diffuse reflectance UV-Vis. The prepared mesoporous titania photocatalyst exhibited significant activity under visible-light irradiation for the degradation of dyes and phenols due to its red shift of band-gap-absorption onset and visible-light response as a result of the incorporation of surface carbon species.

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Section: Photocatalytic Activity Of Mesoporousmentioning

confidence: 87%

Visible-Light Degradation of Dyes and Phenols over Mesoporous Titania Prepared by Using Anthocyanin from Red Radish as Template

Yan

Gong

Chen

et al. 2014

International Journal of Photoenergy

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“…GE has generally replaced crosslinkers such as glutaraldehyde due to advantages such as biocompatibility, well defined chemistry and being less cytotoxic than glutaraldehyde (Muzzarelli, 2009) (Chiono et al, 2008). Further, GE is reported to exhibit pharmacological activity including anti-inflammatory and antibacterial effect (Winotapun et al, 2013) and these together with the biocompatibility make GE relevant for wound healing applications.…”

Section: Introductionmentioning

confidence: 99%

3D printed chitosan dressing crosslinked with genipin for potential healing of chronic wounds

Hafezi

Scoutaris

Douroumis

et al. 2019

International Journal of Pharmaceutics

100

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Graphical Abstract AbstractRecently, various additive manufacturing (3D printing) approaches have been employed to fabricate dressings such as film scaffolds that possess well defined architecture and orientation at the micro level. In this study, crosslinked chitosan (CH) based film matrices were prepared using 3D printing with genipin (GE) as a crosslinker, with glycerol (GLY) and poly ethylene glycol (PEG) as plasticiser. The 3D printed films were functionally characterized (tensile, fluid handling, mucoadhesion, drug dissolution, morphological properties and cell viability as well physico-chemical characterization using scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction. CH-GE-PEG600 3D printed films having the ratio of 1:1 polymer: plasticizer was selected due to their appropriate flexibility. Fourier transform infrared results showed intermolecular interaction between CH, GE and PEG which was confirmed by X-ray diffraction showing amorphous matrix structure. In vitro mucoadhesion studies of CH-GE-PEG600 films showed the capability of the 3D printed film to adhere to the epithelial surface.Scanning electron microscopy images showed that the surface of the plasticised films were smooth indicating content uniformity of CH, GE and PEG whilst micro cracks in unplasticised films confirmed their brittle nature. Plasticised films also showed high swelling capacity which enhanced water absorption. Cytotoxicity (MTT) assay using human skin fibroblast cell lines demonstrated that more than 90% of cells were viable after 48 hours confirming non-toxic nature of the 3D printed CH-GE-PEG600 films and therefore promising dressing for chronic wound healing applications.

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“…Experiments showed that after the crude gardenia fruit was incubated with cellulase (10 mg/mL) at pH 4 for 24 h at 50 • C, in-situ extraction of Genipin with a yield of 58.83 mg/g could be obtained. Compared to the yield obtained by methods that do not require enzymes or in situ extraction, this is an increase of 12.38 and 1.72 times [64]. Chen et al reported a new method to improve flavonoid extraction from ginkgo leaves using Penicillium decumbens cellulase, a commercial cell wall degrading enzyme with high transglycosylation activity which results in better extractions than Trichoderma reesei cellulase and Aspergillus niger pectinase, and can transglycosylate flavonoid aglycones into more polar glucosides.…”

Section: Structural Modification Of Bioactive Ingredients Using Cellulasementioning

confidence: 99%

Current Status of Mining, Modification, and Application of Cellulases in Bioactive Substance Extraction

Kang

Wang

et al. 2021

CIMB

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Cellulases have been used to extract bioactive ingredients from medical plants; however, the poor enzymatic properties of current cellulases significantly limit their application. Two strategies are expected to address this concern: (1) new cellulase gene mining strategies have been promoted, optimized, and integrated, thanks to the improvement of gene sequencing, genomic data, and algorithm optimization, and (2) known cellulases are being modified, thanks to the development of protein engineering, crystal structure data, and computing power. Here, we focus on mining strategies and provide a systemic overview of two approaches based on sequencing and function. Strategies based on protein structure modification, such as introducing disulfide bonds, proline, salt bridges, N-glycosylation modification, and truncation of loop structures, have already been summarized. This review discusses four aspects of cellulase-assisted extraction. Initially, cellulase alone was used to extract bioactive substances, and later, mixed enzyme systems were developed. Physical methods such as ultrasound, microwave, and high hydrostatic pressure have assisted in improving extraction efficiency. Cellulase changes the structure of biomolecules during the extraction process to convert them into effective ingredients with better activity and bioavailability. The combination of cellulase with other enzymes and physical technologies is a promising strategy for future extraction applications.

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One-enzyme catalyzed simultaneous plant cell disruption and conversion of released glycoside to aglycone combined with in situ product separation as green one-pot production of genipin from gardenia fruit

Cited by 28 publications

References 31 publications

Visible-Light Degradation of Dyes and Phenols over Mesoporous Titania Prepared by Using Anthocyanin from Red Radish as Template

Visible-Light Degradation of Dyes and Phenols over Mesoporous Titania Prepared by Using Anthocyanin from Red Radish as Template

3D printed chitosan dressing crosslinked with genipin for potential healing of chronic wounds

Current Status of Mining, Modification, and Application of Cellulases in Bioactive Substance Extraction

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