One-dimensional topography underlies three-dimensional fibrillar cell migration

Doyle, Andrew D.; Wang, Francis W.; Matsumoto, Kazue; Yamada, Katsushi

doi:10.1083/jcb.200810041

Cited by 671 publications

(804 citation statements)

References 26 publications

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“…While pointing at the different roles exerted by the two Hippo effectors on materials with distinct compliances, these results can also be explained by the different nature of 2D and 3D migration, the latter relying on MMP activation. 44,45 As such, these results would confirm the pivotal function of YAP and TAZ in the switch between mesenchymal and amoeboid migration previously suggested. 46 …”

Section: Articlesupporting

confidence: 89%

Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

et al. 2014

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T he Hippo pathway has been recently identified as a crucial axis in the regulation of organ size and shape during organogenesis and cancer. The paralog Yes-associated protein (YAP) and WW domain-containing transcription regulator protein 1 (WWTR1 or TAZ) are the downstream effectors of the Hippo pathway. These proteins have also been identified as mammalian proto-oncogenes. 1 Moreover, they perform as transcriptional coactivators in the nucleus, mainly in combination with transcription factors belonging to TEAD family. 2 In vitro, YAP/TAZ activity has been associated with mesenchymal stem cell (MSC) fate decision through the interaction with key determinants of osteogenic (Runx2) or adipogenic (PPARγ) differentiation. 3,4 YAP/TAZ axis has also emerged as a central regulator of human embryonic stem cell self-renewal through the control of SMAD complex shuttling to the nucleus, with TAZ knock-down resulting in the loss of cell pluripotency. 5 The same cofactors control intestinal 6 and neural progenitor cell number and differentiation 7 by targeting We identify a novel activity of YAP and TAZ in the regulation of tubulogenesis in 3D environments and highlight a role for YAP/TAZ in cardiac progenitor proliferation and differentiation. Furthermore, we show that YAP/TAZ expression is triggered in the heart cells located at the infarct border zone. Our results suggest a fundamental role for the YAP/TAZ axis in the response of resident progenitor cells to the modifications in microenvironment nanostructure and mechanics, thereby contributing to the maintenance of myocardial homeostasis in the adult heart. These proteins are indicated as potential targets to control cardiac progenitor cell fate by materials design.

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Section: Articlesupporting

confidence: 89%

Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

et al. 2014

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“…The differences in cell migration speed on FN coating and fibrillar FN matrix are in agreement with other studies on NIH3T3 fibroblasts and human keratinocytes migration in fibrillar FN [20]. Therefore, the increased migration speed cannot be specifically attributed to cancer cells, but it is rather a general phenomenon, which takes place in this type of extracellular environment.…”

Section: Resultssupporting

confidence: 80%

“…For tumor progression, the relevance of cell migration within an ECM network has been highlighted in studies, which report on qualitative and quantitative differences in migration between 2D and 3D environments [20,21]. Regarding the role of ECM proteins in modulating the interactions between MT1-MMP and integrins, surfaces coated with matrix proteins have been shown to differentially regulate the localization and activity of MT1-MMP, α v β 3 -and β 1 -integrins [20].…”

mentioning

confidence: 99%

“…Regarding the role of ECM proteins in modulating the interactions between MT1-MMP and integrins, surfaces coated with matrix proteins have been shown to differentially regulate the localization and activity of MT1-MMP, α v β 3 -and β 1 -integrins [20]. Fibronectin, which serves as ligand for α v β 3 -and α 5 β 1 -integrins, is an ECM glycoprotein involved in several physiological processes such as embryonic development, cell migration, and vasculogenesis [22].…”

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confidence: 99%

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$$\upalpha 5\upbeta $$ α 5 β 1-integrin and MT1-MMP promote tumor cell migration in 2D but not in 3D fibronectin microenvironments

et al. 2014

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Cell migration is a crucial event for physiological processes, such as embryonic development and wound healing, as well as for pathological processes, such as cancer dissemination and metastasis formation. Cancer cell migration is a result of the concerted action of matrix metalloproteinases (MMPs), expressed by cancer cells to degrade the surrounding matrix, and integrins, the transmembrane receptors responsible for cell binding to matrix proteins. While it is known that cell-microenvironment interactions are essential for migration, the role of the physical state of such interactions remains still unclear. In this study we investigated human fibrosarcoma cell migration in two-dimensional (2D) and three-dimensional (3D) fibronectin (FN) microenvironments. By using antibody blocking approach and cellbinding site mutation, we determined that α 5 β 1 -integrin is Electronic supplementary material The online version of this article (doi:10.1007/s00466-013-0960-6) contains supplementary material, which is available to authorized users. the main mediator of fibrosarcoma cell migration in 2D FN, whereas in 3D fibrillar FN, the binding of α 5 β 1 -and α v β 3 -integrins is not necessary for cell movement in the fibrillar network. Furthermore, while the general inhibition of MMPs with GM6001 has no effect on cell migration in both 2D and 3D FN matrices, we observed opposing effect after targeted silencing of a membrane-bound MMP, namely MT1-MMP. In 2D fibronectin, silencing of MT1-MMP results in decreased migration speed and loss of directionality, whereas in 3D FN matrices, cell migration speed is increased and integrin-mediated signaling for actin dynamics is promoted. Our results suggest that the fibrillar nature of the matrix governs the migratory behavior of fibrosarcoma cells. Therefore, to hinder migration and dissemination of diseased cells, matrix molecules should be directly targeted, rather than specific subtypes of receptors at the cell membrane.

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“…PVA is a scaffold supporting material for tissue engineering applications. It has been frequently utilized for cell culture attachment (Chen et al 2007;Doyle et al 2009;Michaels et al 1995) and as a hydrogel for bioartificial pancreas (Chuang et al 1999;Qi et al 2004). PVA, as a safe component, has been cleared by the United States Food and Drug Administration (FDA) (Lindemann 1971).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and character investigation of new collagen Hydrolysate/polyvinyl alcohol/hydroxyapatite Polymer-Nano-Porous Membranes: I. Experimental design optimization in thermal and structural properties

Imanieh¹,

Aghahosseini²

2013

Syst Synth Biol

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Development of bioorganic-inorganic composites has drawn eyes to extensive attention in biomedical fields and tissue engineering. So many attempts to prepare hydroxyapatite (HA), in conjunction with various binders including polyvinyl alcohol (PVA), and collagen has performed for late 20 years. We applied a method based on the phase separation for making of polymer porous membranes. This procedure is induced through the addition of a small quantity of water (polymer-rich phase) to a solution with HA precursors (polymer-poor phase). Thermal and structural composite properties of collagen Hydrolysate (CH)-PVA/HA Polymer-Nano-Porous Membranes were analyzed by Design of experiment that was undertaken using D-optimal approach, to select the optimal combination of nano composites precursor. The resulted composite characters were investigated by Fourier transform infrared, scanning electron microscopy (SEM) and thermal gravimetric analysis. Based on the SEM images, this new method could be clearly concluded to porous CH-PVA/HA hybrid materials. Finally the hemocompatibility of nanocomposite membranes were evaluated by the hemolysis study.

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One-dimensional topography underlies three-dimensional fibrillar cell migration

Cited by 671 publications

References 26 publications

Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

$$\upalpha 5\upbeta $$ α 5 β 1-integrin and MT1-MMP promote tumor cell migration in 2D but not in 3D fibronectin microenvironments

Synthesis and character investigation of new collagen Hydrolysate/polyvinyl alcohol/hydroxyapatite Polymer-Nano-Porous Membranes: I. Experimental design optimization in thermal and structural properties

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