One-dimensional proteomic profiling of Danio rerio embryo vitellogenin to estimate quantum dot toxicity

Петушкова, Н. А.; Kuznetsova, Galina P.; Ларіна, О. В.; Kisrieva, Yu. S.; Samenkova, N. F.; Trifonova, Oxana P.; Miroshnichenko, Y. V.; Zolotarev, K.V.; Karuzina, I I; Ipatova, O. M.; Лисица, А. В.

doi:10.1186/s12953-015-0072-7

Cited by 8 publications

(5 citation statements)

References 56 publications

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“…Large agglomerates formed and adhered to the chorion of the embryos. This was also reported in other studies ( Sun et al, 2013 ; Petushkova et al, 2015 ; Zheng et al, 2021 ). The agglomerations were the result of increased ZP at the highest exposure concentration and settling out of suspension.…”

Section: Discussionsupporting

confidence: 91%

Influence of different functionalized CdTe quantum dots on the accumulation of metals, developmental toxicity and respiration in different development stages of the zebrafish (Danio rerio)

2023

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Introduction: The bioaccumulation and differential effects of cadmium tellurium quantum dot (CdTe QDs) nanomaterials with different functional groups are poorly understood in aquatic organisms. This study aimed to investigate the metal uptake, developmental effects, and respiratory effects of CdTe QDs with different functional groups (COOH, NH3, and PEG) on zebrafish embryos.Methods: Zebrafish embryos were exposed to carboxylate (COOH), ammonia (NH3), and polyethylene glycol (PEG) functionalized CdTe QDs at nominal concentrations of 0.5, 2, 4, 6, and 20 mg QDs/L. The materials were characterized in E3 exposure media and the metal uptake, developmental effects, and respiratory effects of zebrafish embryos were recorded.Results: The total Cd or Te concentrations in the larvae could not be explained by the metal concentrations or dissolution of the materials in the exposure media. The metal uptake in the larvae was not dose-dependent, except for the QD-PEG treatment. The QD-NH3 treatment caused respiration inhibition at the highest exposure concentration and hatching delays and severe malformations at low concentrations. The toxicities observed at low concentrations were attributed to particles crossing the pores in the chorion, and toxicities at higher concentrations were linked to the aggregation of particle agglomerates to the surface of the chorion impairing respiration. Developmental defects were recorded following exposure to all three functional groups, but the QD-NH3 group had the most severe response. The LC50 values for embryo development of QD-COOH and QD-PEG groups were higher than 20 mg/L, and the LC50 of the QD-NH3 group was 20 mg/L.Discussion: The results of this study suggest that CdTe QDs with different functional groups have differential effects on zebrafish embryos. The QD-NH3 treatment caused the most severe effects, including respiration inhibition and developmental defects. These findings provide valuable information for understanding the effects of CdTe QDs on aquatic organisms and highlight the need for further investigation.

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Section: Discussionsupporting

confidence: 91%

Influence of different functionalized CdTe quantum dots on the accumulation of metals, developmental toxicity and respiration in different development stages of the zebrafish (Danio rerio)

2023

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“…It is a lipoglycoprotein that is employed as a vehicle to provide the developing embryo with proteins, lipids, carbohydrates, and other essential resources. VTG2 was increased by ZnO-NP-5μg/mL however not changed in ZnSO 4 -10μg/mL group which further suggested NPs specifically regulated protein expression [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Differential Regulation of Gene and Protein Expression by Zinc Oxide Nanoparticles in Hen’s Ovarian Granulosa Cells: Specific Roles of Nanoparticles

Zhao

Li²,

Zhang

et al. 2015

PLoS ONE

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Annually, tons and tons of zinc oxide nanoparticles (ZnO NPs) are produced in the world. And they are applied in almost all aspects of our life. Their release from the products into environment may pose issue for human health. Although many studies have reported the adverse effects of ZnO NPs on organisms, little is known about the effects on female reproductive systems or the related mechanisms. Quantitative proteomics have not been applied although quantitative transcriptomics have been used in zinc oxide nanoparticles (ZnO NPs) research. Genes are very important players however proteins are the real actors in the biological systems. By using hen’s ovarian granulosa cells, it was found that ZnO-NP-5μg/ml and ZnSO4-10μg/ml treatments produced the same amount of intracellular Zn and resulted in similar cell growth inhibition. And NPs were found in the treated cells. However, ZnO-NP-5μg/ml specifically regulated the expression of genes and proteins compared with that in ZnSO4-10μg/ml treatment. For the first time, this investigation reports that intact NPs produce different impacts on the expression of genes and proteins involved in specific pathways compared to that by Zn2+. The findings enrich our knowledge for the molecular insights of zinc oxide nanoparticles effects on the female reproductive systems. This also may raise the health concern that ZnO NPs may adversely affect the female reproductive systems through regulation of specific signaling pathways.

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“…Several factors have inhibited analysis of the correspondence between changes in mRNA expression and consequent protein expression changes. There is a dearth of commercially available antibodies that detect zebrafish proteins and proteomic analyses of zebrafish embryos can be problematic due to interference from the high levels of yolk vitellogenins present at these stages [112]. Hopefully, the results of our analyses will direct future proteomic experimentation in cultured human cells that will support our findings.…”

Section: Validation Of Network Nodes (Hubs) By Rt-qpcrmentioning

confidence: 64%

The Zebrafish Equivalent of Alzheimer’s Disease-Associated PRESENILIN Isoform PS2V Regulates Inflammatory and Other Responses to Hypoxic Stress

Ebrahimie

Nik

Newman

et al. 2016

JAD

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Dominant mutations in the PRESENILIN genes PSEN1 and PSEN2 cause familial Alzheimer's disease (fAD) that usually shows onset before 65 years of age. In contrast, genetic variation at the PSEN1 and PSEN2 loci does not appear to contribute to risk for the sporadic, late onset form of the disease (sAD), leading to doubts that these genes play a role in the majority of AD cases. However, a truncated isoform of PSEN2, PS2V, is upregulated in sAD brains and is induced by hypoxia and high cholesterol intake. PS2V can increase γ-secretase activity and suppress the unfolded protein response (UPR), but detailed analysis of its function has been hindered by lack of a suitable, genetically manipulable animal model since mice and rats lack this PRESENILIN isoform. We recently showed that zebrafish possess an isoform, PS1IV, that is cognate to human PS2V. Using an antisense morpholino oligonucleotide, we can block specifically the induction of PS1IV that normally occurs under hypoxia. Here, we exploit this ability to identify gene regulatory networks that are modulated by PS1IV. When PS1IV is absent under hypoxia-like conditions, we observe changes in expression of genes controlling inflammation (particularly sAD-associated IL1B and CCR5), vascular development, the UPR, protein synthesis, calcium homeostasis, catecholamine biosynthesis, TOR signaling, and cell proliferation. Our results imply an important role for PS2V in sAD as a component of a pathological mechanism that includes hypoxia/oxidative stress and support investigation of the role of PS2V in other diseases, including schizophrenia, when these are implicated in the pathology.

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One-dimensional proteomic profiling of Danio rerio embryo vitellogenin to estimate quantum dot toxicity

Cited by 8 publications

References 56 publications

Influence of different functionalized CdTe quantum dots on the accumulation of metals, developmental toxicity and respiration in different development stages of the zebrafish (Danio rerio)

Influence of different functionalized CdTe quantum dots on the accumulation of metals, developmental toxicity and respiration in different development stages of the zebrafish (Danio rerio)

Differential Regulation of Gene and Protein Expression by Zinc Oxide Nanoparticles in Hen’s Ovarian Granulosa Cells: Specific Roles of Nanoparticles

The Zebrafish Equivalent of Alzheimer’s Disease-Associated PRESENILIN Isoform PS2V Regulates Inflammatory and Other Responses to Hypoxic Stress

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