One‐day pulsed‐field gel electrophoresis protocol for rapid determination of emetic <i>Bacillus cereus</i> isolates

Kaminska, Paulina S.; Fiedoruk, Krzysztof; Jankowska, Dominika; Mahillon, Jacques; Nowosad, Karol; Drewicka, Ewa; Zambrzycka, Monika; Święcicka, Izabela

doi:10.1002/elps.201400552

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…Based on the results obtained, the recovered isolates were subjected to different phenotypic and genotypic characterization tests, including antimicrobial susceptibility testing [ 17 ], serotyping using the Kauffmann-White Salmonella scheme, pulse-field-gel electrophoresis (PFGE) of Bacillus cereus with the Not I macrorestriction enzyme according to the procedure previously described [ 18 ] and whole-genome sequencing on the Illumina MiSeq platform (Illumina, San Diego, CA, USA). The assembled genomes were analyzed with the BTyper tool (version 2.3.2) and PATRIC workspace ( https://www.patricbrc.org/ ) for the identification of MLST profiles, phylogenetic relationships, antimicrobial genes and virulence factors.…”

Section: Methodsmentioning

confidence: 99%

“…of Bacillus cereus with the NotI macrorestriction enzyme according to the procedure previously described [18] and whole-genome sequencing on the Illumina MiSeq platform (Illumina, San Diego, CA, USA). The assembled genomes were analyzed with the BTyper tool (version 2.3.2) and PATRIC workspace (https://www.patricbrc.org/) for the identification of MLST profiles, phylogenetic relationships, antimicrobial genes and virulence factors.…”

Section: Plos Onementioning

confidence: 99%

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Infections in patients with adverse reactions to the use of unknown modeling substances for soft tissue enhancement in Cali, Colombia

Moncada

Ríos²,

Castro³

et al. 2023

PLoS ONE

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The infiltration of foreign materials not approved for medical purposes or of modeling substances used in soft tissue to modify the anatomical appearance for aesthetic purposes represents a serious health problem. These procedures lead to the development of delayed complications, including infections. The objective of this study was to characterize infections in patients with adverse reactions to the use of modeling substances in Cali, Colombia. A cross-sectional and descriptive study was used to determine the frequency of bacterial and fungal infections associated with complications from and adverse reactions to the use of modeling substances in 113 patients. We identified microorganisms in 22 patients and a frequency of 68.1% monomicrobial infections and 31.8% polymicrobial infections. The microorganisms identified in our study included Bacillus cereus, Mycobacterium fortuitum, and Pseudomonas stutzeri, among other microorganisms. The presence of adverse effects derived from the use of illegal modeling substances has been demonstrated; among these effects, infections occur with high frequency and place the health of the patient at risk and increase problems in health care.

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Section: Methodsmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Infections in patients with adverse reactions to the use of unknown modeling substances for soft tissue enhancement in Cali, Colombia

Moncada

Ríos²,

Castro³

et al. 2023

PLoS ONE

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“…Values below this cutoff strongly support presence of distinct individual species. Finally, an approach of sequencing several housekeeping genes (technique named MLST, multi-locus sequence typing) proved that phylogenetic structure of B. cereus sensu lato corresponds better to their ecological properties than to taxonomic affiliation (Święcicka et al, 2013;Drewnowska and Święcicka 2013;Kamińska et al, 2015). For example, cold adapted bacteria (syn.…”

Section: Science In Short Schoolmentioning

confidence: 99%

Bacterial species – from theory to practice

Bartoszewicz¹

2018

EBIS

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Although the Mayr's definition of species is commonly accepted for eukaryotic organisms, bacteria do not meet those established criteria. Thus, a special approach is necessary to elaborate the definition resistant to such limitations as for example the lack of sexual reproduction or horizontal gene transfers. One of the most problematic taxa in microbiology is the Bacillus cereus group composed of several closely related bacteria. Thus, on the basis of this model, doubts concerning bacterial taxonomy are discussed in the light of actual, molecular data.

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“…The “gold standard” of emetic B. cereus detection (quantitative polymerase chain reaction (qPCR)) and other methods, such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and pulsed-field gel electrophoresis, depend on expensive and cumbersome instruments and cannot rapidly obtain results. − The aforementioned drawbacks can be avoided by using isothermal amplification techniques like loop-mediated isothermal amplification (LAMP) . However, the complex design of primers for LAMP resulted in high false positives, which limit its application in the on-site detection of emetic B. cereus .…”

Section: Introductionmentioning

confidence: 99%

Universal and Naked-Eye Diagnostic Platform for Emetic Bacillus cereus Based on RPA-Assisted CRISPR/Cas12a

Meng,

Xu,

et al. 2024

J. Agric. Food Chem.

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Emetic Bacillus cereus (B. cereus), which can cause emetic food poisoning and in some cases even fulminant liver failure and death, has aroused widespread concern. Herein, a universal and naked-eye diagnostic platform for emetic B. cereus based on recombinase polymerase amplification (RPA)-assisted CRISPR/Cas12a was developed by targeting the cereulide synthetase biosynthetic gene (cesB). The diagnostic platform enabled one-pot detection by adding components at the bottom and cap of the tube separately. The visual limit of detection of RPA-CRISPR/Cas12a for gDNA and cells of emetic B. cereus was 10 −2 ng μL −1 and 10 2 CFU mL −1 , respectively. Meanwhile, it maintained the same sensitivity in the rice, milk, and cooked meat samples even if the gDNA was extracted by simple boiling. The whole detection process can be finished within 40 min, and the single cell of emetic B. cereus was able to be recognized through enrichment for 2−5 h. The good specificity, high sensitivity, rapidity, and simplicity of the RPA-assisted CRISPR/Cas12a diagnostic platform made it serve as a potential tool for the on-site detection of emetic B. cereus in food matrices. In addition, the RPA-assisted CRISPR/Cas12a assay is the first application in emetic B. cereus detection.

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One‐day pulsed‐field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates

Cited by 5 publications

References 17 publications

Infections in patients with adverse reactions to the use of unknown modeling substances for soft tissue enhancement in Cali, Colombia

Infections in patients with adverse reactions to the use of unknown modeling substances for soft tissue enhancement in Cali, Colombia

Bacterial species – from theory to practice

Universal and Naked-Eye Diagnostic Platform for Emetic Bacillus cereus Based on RPA-Assisted CRISPR/Cas12a

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