One-Carbon Metabolism Links Nutrition Intake to Embryonic Development via Epigenetic Mechanisms

Wu, Siyu; Zhang, Jun; Li, Feifei; Du, Wei; Zhou, Xin; Wan, Mei-Hua; Fan, Yi; Xu, Xin; Zhou, Xuedong; Zheng, Liwei; Zhou, Yachuan

doi:10.1155/2019/3894101

Cited by 20 publications

(13 citation statements)

References 94 publications

(98 reference statements)

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“…Methyl donor insufficiency constitutes a risk factor for weaned pups in growth retardation, delayed ossification, and cognitive deficits (14). Previous studies have mainly focused on the impact of methyl donor on neural tube defect, metabolism, and human and mouse offspring diseases (15)(16)(17). Recently, some studies have shown that the accumulation of methyl donor in cells is beneficial in improving muscle mass and betaine has a significant effect on skeletal myogenesis, such as proliferation and differentiation of myoblast in vitro and in vivo (18).…”

Section: Introductionmentioning

confidence: 99%

Maternal Methyl-Donor Micronutrient Supplementation During Pregnancy Promotes Skeletal Muscle Differentiation and Maturity in Newborn and Weaning Pigs

Zou

et al. 2020

Front. Nutr.

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Adequate maternal methyl-donor micronutrient (MET) intake is an important determinant of the organ development and metabolic renovation of offspring. The mechanism involved in skeletal myogenesis and the effect of MET supplementation during pregnancy on the maternal body remain unclear. Thus, this study aimed to investigate the potential effect of methyl donor micronutrients (MET) on skeletal muscle development and metabolism in offspring using pig models. Forty-three Duroc × Erhualian gilts were assigned to two dietary groups during gestation: control diet (CON) and CON diet supplemented with MET (folic acid, methionine, choline, vitamin B6, and vitamin B12). The results showed that maternal MET exposure during pregnancy significantly increased the concentrations of protein, triiodothyronine (T3), and thyroxine (T4) in colostrum and methyl metabolites, including S-adenosylmethionine (SAM), S-adenosyl-L-homocysteine (SAH), 5-methyl-tetrahydrofolate (5-MTHF), and betaine, in the maternal and offspring umbilical vein serum. A similar pattern was demonstrated in the body weight gain and myofiber diameters in offspring. In addition, maternal MET supplementation significantly increased the concentration of offspring serum insulin-like growth factor 1 (IGF-1), T3, and T4; upregulated the mRNA expression of IGF-1 and IGF-1 receptor (IGF-1r) and the phosphorylation level of protein kinases in offspring longissimus dorsi muscle; and upregulated the expression of myogenic genes and fast myosin heavy chain (fast MyHC) in offspring skeletal muscle. Supplementing sows with higher levels of MET during gestation may promote skeletal muscle differentiation and maturity and improve the skeletal muscle mass of the piglets.

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Section: Introductionmentioning

confidence: 99%

Maternal Methyl-Donor Micronutrient Supplementation During Pregnancy Promotes Skeletal Muscle Differentiation and Maturity in Newborn and Weaning Pigs

Zou

et al. 2020

Front. Nutr.

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“…The altered methyl donor metabolism affects DNA methylation, histone modifications, and miRNAs. In addition, methyl donor challenges in humans, such as a LPD, alters epigenetically programed ES cell progenitor differentiation (Wu et al, 2019).…”

Section: Methyl Donor Perturbations Including a Maternal Low Proteinmentioning

confidence: 99%

“…Other locations within the human genome may be affected by changes in epigenetic marks as the result of diet fluctuations. For example, active H3K4me3 and repressive H3K27me3 maintain a pluripotent epigenetic state in ES and their progenitor cells (Wu et al, 2019). In hES cells, there are also abundant non-CpG methylated cytosines that may be important to early development (Lister et al, 2009).…”

Section: Methyl Donor Perturbations Including a Maternal Low Proteinmentioning

confidence: 99%

One-Carbon Metabolism Regulates Embryonic Stem Cell Fate Through Epigenetic DNA and Histone Modifications: Implications for Transgenerational Metabolic Disorders in Adults

Winkle

Ryznar

2019

Front. Cell Dev. Biol.

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Human (h) and mouse (m) embryonic stem (ES) cells need specific amino acids to proliferate. mES cells require threonine (Thr) metabolism for epigenetic histone modifications. Thr is converted to glycine and acetyl CoA, and the glycine is metabolized specifically to regulate trimethylation of lysine (Lys) residue 4 in histone H3 (H3K4me3). DNA methylation and methylation of other H3 Lys residues remain unimpaired by Thr deprivation in mES cell culture medium. Similarly, hES cells require methionine (Met) to maintain the Met-SAM (S-adenosyl methionine) cycle of 1-carbon metabolism also for H3K4me3 formation. H3K4me3 is needed specifically to regulate and maintain both mES and hES cell proliferation and their pluripotent states. Better understanding of this regulation is essential since treatment of human diseases and disorders will increasingly involve hES cells. Furthermore, since ES cells are derived from their progenitor cells in preimplantation blastocysts, they serve as models of 1-carbon metabolism in these precursors of all mammalian tissues and organs. One-carbon metabolism challenges, such as a maternal low protein diet (LPD) during preimplantation blastocyst development, contribute to development of metabolic syndrome and related abnormalities in adults. These 1-carbon metabolism challenges result in altered epigenetic DNA and histone modifications in ES progenitor cells and the tissues and organs to which they develop. Moreover, the modified histones could have extracellular as well as intracellular effects, since histones are secreted in uterine fluid and influence early embryo development. Hence, the mechanisms and transgenerational implications of these altered epigenetic DNA and histone modifications warrant concerted further study.

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“…The methyl groups from one‐carbon metabolism can be utilized in different ways, including DNA methylation, histone modification and microRNA. [ 109 ] As shown in Figure 5 , SAM metabolized by methionine is the major methyl donor in cells and can transfer methyl groups to histones, DNA, RNA, and lysine and arginine residues in proteins. [ 110–112 ] Changes in the SAM level in cells affect methyltransferase activity and the histone methylation level.…”

Section: Functions Of One Carbon Metabolism In Mammals During Pregnancymentioning

confidence: 99%

One Carbon Metabolism and Mammalian Pregnancy Outcomes

Cai

Shuang

Gao

et al. 2020

Molecular Nutrition Food Res

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One‐carbon metabolism is involved in varieties of physiological processes in mammals, including nucleic acid synthesis, amino acid homeostasis, epigenetic regulation, redox balance and neurodevelopment. The current evidence linking levels of one‐carbon nutrients during pregnancy to the development of oocytes, embryos, and placentas, as well as maternal and offspring health, is reviewed. The sources of mammalian one‐carbon units, the pathways active in mammalian one‐carbon metabolism, the maternal and fetal needs for one‐carbon units and their functions during pregnancy are described. The demand for one‐carbon metabolism is highest during pregnancy compared to the entire lifetime of a mammal. The primary types of one‐carbon metabolism in mammals are the folate cycle, methionine cycle and transsulfuration pathway, which varies at different pregnancy stages (e.g., methylation programming of embryo, neural development of fetus, fetal growth and placenta development). Therefore, an overall consideration of one‐carbon metabolism requirements for different pregnancy stages, is called for, specifically, the balance of all nutrients involved, not just one single nutrient in one‐carbon metabolism. Moreover, the establishment of an ideal one‐carbon metabolism requirement model is suggested according to the requirements for different pregnancy stages to support optimal pregnancy outcomes and maternal and offspring health.

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One-Carbon Metabolism Links Nutrition Intake to Embryonic Development via Epigenetic Mechanisms

Cited by 20 publications

References 94 publications

Maternal Methyl-Donor Micronutrient Supplementation During Pregnancy Promotes Skeletal Muscle Differentiation and Maturity in Newborn and Weaning Pigs

Maternal Methyl-Donor Micronutrient Supplementation During Pregnancy Promotes Skeletal Muscle Differentiation and Maturity in Newborn and Weaning Pigs

One-Carbon Metabolism Regulates Embryonic Stem Cell Fate Through Epigenetic DNA and Histone Modifications: Implications for Transgenerational Metabolic Disorders in Adults

One Carbon Metabolism and Mammalian Pregnancy Outcomes

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