Oncometabolite Fingerprinting Using Fluorescent Single‐Walled Carbon Nanotubes (Adv. Mater. Interfaces 4/2022)

Amir, Dean; Hendler‐Neumark, Adi; Wulf, Verena; Ehrlich, Roni; Bisker, Gili

doi:10.1002/admi.202270019

Cited by 4 publications

(5 citation statements)

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“…[ 22,23 ] SWCNTs are graphene‐based nanostructures, where different rolling orientations of graphene sheets give rise to a wide range of nanotube chiralities, [ 24,25 ] each of which has unique physical, chemical, and optical properties. [ 26–29 ] Semiconducting SWCNTs fluoresce in the near‐infrared (NIR) range, which overlaps with the transparency window of biological samples, thus can be utilized for biotechnological and biomedical imaging and sensing applications, [ 23,30–37 ] including sensing of small molecules, [ 38–44 ] reactive oxygen species, [ 45–47 ] microRNA, [ 48 ] lipids, [ 49 ] proteins, [ 50–54 ] enzymes, [ 55–58 ] volatiles, [ 59,60 ] and pathogens. [ 61,62 ] While previously, DNA functionalized fluorescent SWCNTs were utilized to detect arsenite within plants, [ 63 ] most SWCNT applications as metal sensors are based on oxidized (non‐fluorescent) SWCNTs and do not exploit their optical properties.…”

Section: Introductionmentioning

confidence: 99%

Multicomponent System of Single‐Walled Carbon Nanotubes Functionalized with a Melanin‐Inspired Material for Optical Detection and Scavenging of Metals

Wulf

Bichachi

Hendler‐Neumark

et al. 2022

Adv Funct Materials

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The accumulation of metal ions in organisms and the presence of heavy metals in water cause adverse effects on ecosystems and results in numerous human health issues such as cancer and neurogenerative diseases. Therefore, the development of novel platforms for metal‐scavenging and rapid metal detection for in situ applications are of high importance. Here, this challenge is tackled by taking advantage of the metal chelation ability of a melanin‐inspired material in combination with the near‐infrared (NIR) fluorescence response of single‐walled carbon nanotubes (SWCNTs) to surface binding. SWCNTs are functionalized by a melanin‐like substance, obtained by enzymatic oxidative polymerization of a fluorenylmethyloxycarbonyl‐tyrosine (FmocY) precursor. The resulting multicomponent system (SWCNT‐FmocYOx) serves as a metal‐ion scavenging platform that concurrently reports on metal binding with optical signal transduction. Upon binding of a library of mostly divalent transition metal‐ions, the fluorescence emission of the functionalized SWCNTs is modulated, showing a concentration‐dependent response with a limit of detection in the nanomolar range. Metal‐binding and removal from water of up to 98% is further shown via inductively coupled plasma mass spectrometry. The SWCNT‐FmocYOx hybrid system presents a novel platform with NIR optical signal for real‐time feedback on metal‐ion scavenging.

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Section: Introductionmentioning

confidence: 99%

Multicomponent System of Single‐Walled Carbon Nanotubes Functionalized with a Melanin‐Inspired Material for Optical Detection and Scavenging of Metals

Wulf

Bichachi

Hendler‐Neumark

et al. 2022

Adv Funct Materials

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“…[ 42–59 ] A compelling avenue involves the utilization of single‐walled carbon nanotubes (SWCNTs), which offer several advantageous features such as remarkable photostable fluorescence emission in the NIR range, biocompatibility, and ease of surface functionalization. [ 60–70 ] SWCNTs have been used for fluorometric detection of important analytes, including small molecules, [ 53,71–74 ] oncometalbolites, [ 75 ] proteins, [ 60,61,68,69,76–80 ] neurotransmitters, [ 42,81–83 ] pathogens, [ 84,85 ] metal ions, [ 86 ] endolysosomal pH, [ 87 ] disease biomarkers, [ 88,89 ] plant hormones, [90] sugars, [91] quaternary ammonium compounds, [ 92 ] microRNA [ 93–95 ] cytokines, [ 79 ] volatiles, [ 96,97 ] hormones, [ 90,98 ] and bacterial siderophores, [60] to name a few. In addition, SWCNTs have been utilized as a probe for the inactivation of tyrosinase enzyme by observing a bathochromic shift in their emission wavelength, induced by the generation of singlet oxygen during the enzyme inactivation process.…”

Section: Introductionmentioning

confidence: 99%

Rationally Designed Functionalization of Single‐Walled Carbon Nanotubes for Real‐Time Monitoring of Cholinesterase Activity and Inhibition in Plasma

Basu,

Hendler‐Neumark,

Bisker

2024

Small

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Enzymes play a pivotal role in regulating numerous bodily functions. Thus, there is a growing need for developing sensors enabling real‐time monitoring of enzymatic activity and inhibition. The activity and inhibition of cholinesterase (CHE) enzymes in blood plasma are fluorometrically monitored using near‐infrared (NIR) fluorescent single‐walled carbon nanotubes (SWCNTs) as probes, strategically functionalized with myristoylcholine (MC)– the substrate of CHE. A significant decrease in the fluorescence intensity of MC‐suspended SWCNTs upon interaction with CHE is observed, attributed to the hydrolysis of the MC corona phase of the SWCNTs by CHE. Complementary measurements for quantifying choline, the product of MC hydrolysis, reveal a correlation between the fluorescence intensity decrease and the amount of released choline, rendering the SWCNTs optical sensors with real‐time feedback in the NIR biologically transparent spectral range. Moreover, when synthetic and naturally abundant inhibitors inhibit the CHE enzymes present in blood plasma, no significant modulations of the MC‐SWCNT fluorescence are observed, allowing effective detection of CHE inhibition. The rationally designed SWCNT sensors platform for monitoring of enzymatic activity and inhibition in clinically relevant samples is envisioned to not only advance the field of clinical diagnostics but also deepen further understanding of enzyme‐related processes in complex biological fluids.

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“…[105][106][107][108][109][110][111][112] Several studies demonstrated the feasibility of SWCNTs as fluorescence sensors or markers in vivo. [79][80][81][113][114][115][116][117][118][119][120][121][122][123] Indeed, imaging C. elegans using the fluorescence emission of SWCNTs can be advantageous owing to the spectral distinguishability between the SWCNTs' NIR fluorescence and the autofluorescence of the worm in the visible range, thereby improving quantitative analysis as well. Hence, the utilization of SWCNTs as fluorescence probes can be extended beyond specific areas or organs.…”

Section: Introductionmentioning

confidence: 99%

“…[ 105–112 ] Several studies demonstrated the feasibility of SWCNTs as fluorescence sensors or markers in vivo. [ 79–81,113–123 ]…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Tracking of Near‐Infrared Fluorescent Single‐Walled Carbon Nanotubes in C. Elegans Nematodes Confined in a Microfluidics Platform

Sharaga,

Hendler‐Neumark,

Kamber

et al. 2023

Adv Materials Technologies

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Caenorhabditis elegans (C. elegans) nematodes are a powerful model organism for diverse biological and biomedical studies, benefiting from their genetic similarities to humans, small size, and transparency. However, fluorescence imaging of C. elegans can be challenging due to the strong autofluorescence in the visible range, which obscures the signal of common fluorescent proteins or dyes. Single‐walled carbon nanotubes (SWCNTs) fluoresce in the near‐infrared (NIR) range, where there is no autofluorescence background. Herein, a platform is developed for in vivo NIR imaging of C. elegans gastrointestinal tract using biocompatible SWCNTs functionalized by single‐stranded DNA or phospholipid‐polyethylene glycol (PEG). The SWCNTs serve as fluorescent tracking probes within the worm gut, following internalization along with food intake. A microfluidic confinement device is employed to ensure an anesthetics‐free feeding environment, allowing spatiotemporal control over the SWCNTs intake imaging. Furthermore, improvements in spectral colocalization, real‐time detection of intracorporeal SWCNT dynamics, and digestive trajectory tracking are demonstrated. Owing to the unique optical properties of SWCNTs and the confinement of the worms in the microfluidics system, the proposed platform facilitates advanced in vivo imaging of C. elegans in both the visible and NIR regions, opening numerous avenues for advancing research of C. elegans and other microscopic model organisms.

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Oncometabolite Fingerprinting Using Fluorescent Single‐Walled Carbon Nanotubes (Adv. Mater. Interfaces 4/2022)

Cited by 4 publications

References 0 publications

Multicomponent System of Single‐Walled Carbon Nanotubes Functionalized with a Melanin‐Inspired Material for Optical Detection and Scavenging of Metals

Multicomponent System of Single‐Walled Carbon Nanotubes Functionalized with a Melanin‐Inspired Material for Optical Detection and Scavenging of Metals

Rationally Designed Functionalization of Single‐Walled Carbon Nanotubes for Real‐Time Monitoring of Cholinesterase Activity and Inhibition in Plasma

Spatiotemporal Tracking of Near‐Infrared Fluorescent Single‐Walled Carbon Nanotubes in C. Elegans Nematodes Confined in a Microfluidics Platform

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