On x(ax+ 1)+y(by+ 1)+z(cz+ 1) and x(ax+b)+y(ay+c)+z(az+d)

Sun, Zhi-Wei

doi:10.1016/j.jnt.2016.07.024

Cited by 75 publications

(83 citation statements)

References 6 publications

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“…pDNA: plasmid DNA; TAT: transactivator of transcription [Color figure can be viewed at wileyonlinelibrary.com] disorders. To determine whether siRNA can be delivered by hPP10, hPP10-E3 (E3, c-terminal double-stranded RNA binding domain from Vaccinia virus [Ostertag, Hoblitzell-Ostertag, & Perrault, 2007]), and TAT-HA-hADR1 (hADR1, double-stranded RNA binding domain from human adenosine deaminase [Sun et al, 2015;Washburn et al, 2014]) fusion protein encoding plasmids were constructed (Supporting Information Figure S3), and the fusion protein was prepared (Figure 6a and 6b). After G418 screening, the GFP-expressing stable Caski cell line was established for further hPP10-mediated siRNA transfection analysis (Supporting Information Figure S4).…”

Section: Hpp10 Mediated Sirna Transfectionmentioning

confidence: 99%

Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide

Ding

Zhao

Geng

et al. 2018

Journal Cellular Physiology

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Although gene therapy offers hope against incurable diseases, nonreplicating transduction vectors remain lacking. We have previously characterized a cell‐penetrating peptide hPP10 for the delivery of various cargoes; however, whether hPP10 can mediate nucleic acid delivery is still unknown. Here, examining via different ways, we demonstrate that hPP10 stably complexes with plasmid DNA (pDNA) and safely mediates nucleic acid transfection. hPP10 can mediate GFP‐, dsRed‐, and luciferase‐expressing plasmids into cells with nearly the same efficiency as commercial transfection reagents Turbofectin or Lipofect. Furthermore, hPP10 can mediate Cre fusion protein delivery and pDNA transfection simultaneously in the Cre/loxp system in vitro. In addition, hPP10 fused with an RNA‐binding domain can mediate delivery of small interfering RNA into cells to silence the reporter gene expression. Collectively, our results suggest that hPP10 is an option for nucleic acid delivery with efficiencies similar to that of commercial reagents.

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Section: Hpp10 Mediated Sirna Transfectionmentioning

confidence: 99%

Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide

Ding

Zhao

Geng

et al. 2018

Journal Cellular Physiology

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“…In pavement material research, WCO has usually been blended into asphalt to replace or modify the asphalt with improved properties. 14 Some studies have indicated that waste oil can improve the low-temperature performance of asphalt binders and the thermal cracking resistance, [15][16][17] but decrease the rutting resistance of asphalt. [18][19][20][21][22] Existing studies have proved that WCO can be used as an additive in asphalt.…”

Section: Introductionmentioning

confidence: 99%

Investigation of physiochemical and rheological properties of waste cooking oil/SBS/EVA composite modified petroleum asphalt

Liu

Zhou²,

Peng³

et al. 2019

J of Applied Polymer Sci

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The objective of this research is to investigate the physiochemical and rheological properties of waste cooking oil (WCO)/styrene‐b‐butadiene‐b‐styrene/ethylene vinyl acetate (SBS/EVA) composite modified asphalt. A petroleum asphalt composite modified with SBS/EVA and oil/SBS/EVA were prepared to undergo the physical, rheological, and microscopic experiments. Comparing these tests results with the properties of unmodified petroleum asphalt, SBS and EVA cause an improvement in pavement performance, but weaken the storage stability. Although the high‐temperature performance undermined by the addition of WCO, the thermal stability, fatigue property, and low temperature crack resistance improved obviously. In addition, WCO mainly composed by low‐weight components, and these can supplement the light components in the polymer‐modified asphalt. EVA could increase the percentage of large molecular size of asphalt, while WCO relieves this trend. The reactions between WCO and these polymers (SBS and EVA) are physical reactions. Atomic force microscopy morphology results reflect that SBS and EVA increase the surface complexity and roughness, and WCO makes the asphalt surface smooth. This indicates WCO increases the dispersion of polymers in asphalt. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48828.

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“…[66] Also, MqsR/MqsA impacts biofilm formation in Pseudomonas fluorescens [67] and impacts persistence and biofilm formation in Pseudomonas putida. [68] In addition, deletion of five TA systems was found to alter biofilm formation by affecting fimbriae production through TabA. [69] Therefore, TA systems have a clear role in biofilm formation.…”

Section: Wwwadv-biosyscom Wwwadvancedsciencenewscommentioning

confidence: 99%

Toxin/Antitoxin System Paradigms: Toxins Bound to Antitoxins Are Not Likely Activated by Preferential Antitoxin Degradation

Song

Wood

2020

Adv. Biosys.

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Periodically, a scientific field should examine its early premises. For ubiquitous toxin/antitoxin (TA) systems, several initial paradigms require adjustment based on accumulated data. For example, it is now clear that under physiological conditions, there is little evidence that toxins of TA systems cause cell death and little evidence that TA systems cause persistence. Instead, TA systems are utilized to reduce metabolism during stress, inhibit phages, stabilize genetic elements, and influence biofilm formation (bacterial cells attached via an extracellular matrix). In this essay, it is argued that toxins bound to antitoxins are not likely to become activated by preferential antitoxin degradation but instead, de novo toxin synthesis in the absence of stoichiometric amounts of antitoxin activates toxins.

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On x(ax+ 1)+y(by+ 1)+z(cz+ 1) and x(ax+b)+y(ay+c)+z(az+d)

Abstract: Abstract. In this paper we first investigate for what positive integers a, b, c every nonnegative integer n can be written as x(ax + 1) + y(by + 1) + z(cz + 1) with x, y, z integers. We show that (a, b, c) can be either of the following seven triples

Cited by 75 publications

References 6 publications

Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide

Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide

Investigation of physiochemical and rheological properties of waste cooking oil/SBS/EVA composite modified petroleum asphalt

Toxin/Antitoxin System Paradigms: Toxins Bound to Antitoxins Are Not Likely Activated by Preferential Antitoxin Degradation

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