On “Weed Science” Vol. 16

邦和, 植木; 昭一, 松中; 拓, 中村

doi:10.3719/weed.1969.9_57

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The addition of DC to the DAB medium brought about a slight suppression of the DAB photooxidation. The addition of one of the PS-II inhibitors (26) to the medium did not cause any inhibitory effect on the photooxidation at all, but showed a slight stimulatory one. Supplementing the PS-II activity to the medium by adding DPIP and ASC in the presence of DCMU or DCPA (13) resulted in a marked inhibition of the photooxidation.…”

Section: Discussionmentioning

confidence: 99%

Cytochemical study of diaminobenzidine oxidation by isolated Vicia chloroplasts under illumination.

Imaizumi

Hiraoka

1982

Acta Histochem. Cytochem

View full text Add to dashboard Cite

A concurrent microchemical and topochemical study of DAB* oxidation by isolated chloroplasts of Vicia faba under illumination was carried out. A new method for the quantification of the oxidation product of DAB was explored.The microchemical analysis by this method revealed that the photosystem responsible for the DAB oxidation was the PS-I. It also proved that endogenous active oxygen in the chloroplasts played only a little role in the oxidation.The topochemical analysis revealed that both the grana and the stroma membrane were positive in DAB photooxidation. The densitometric traces across the grana membranes in both surface and side views proved effective in locating the precise reaction sites.The partition layer showed a high degree of positive reaction.The thylakoid membrane proper also contained particles of positive reaction. The positive site extended to the thylakoid loculus to form protrusions.These results are in harmony with the localization of the PS-I complex particles on the PF face side of the thylakoid membrane as revealed by the freeze fracture technique.The concurrent microchemical and topochemical study of tetrazolium reduction by isolated Vicia chloroplasts under illumination has demonstrated that the photosystem responsible for the reduction is the PS-II*. It also revealed that the electron transfer activity mediated by this system is located in the thylakoid membrane proper, and extends to the surface of the loculus (12). Similar cytochemical study along this line was undertaken of the DAB oxidation by isolated Vicia chloroplasts under illumination in order to get some insight into the following three points : first, the photosystem responsible for the oxidation reaction under illumination, secondly, the possible role of endogenous active oxygen in the reaction, and thirdly, the localization of the photosystem concerned in the thylakoid membrane. This paper deals with the results obtained.

show abstract

Section: Discussionmentioning

confidence: 99%