On tight-junction structure

Silva, Pedro Pinto da; Kachar, Bechara

doi:10.1016/0092-8674(82)90198-2

Cited by 213 publications

(86 citation statements)

References 34 publications

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“…Although it was most likely that the intramembranous particles in the freeze-fracture replica samples reflected the integral membrane proteins, the real nature of the TJ strands, lipids and/or proteins remained a mystery until the proteinaceous components constituting the TJ strands were identified (Kachar and Reese, 1982;Pinto da Silva and Kachar, 1982;Verkleij, 1984). Hence, when a novel integral membrane protein with four transmembrane domains of B60 kDa, termed occludin (Furuse et al, 1993), was identified as a component of TJ strands from hepatic junctional fractions (Tsukita and Tsukita, 1989), it was regarded as the Holy Grail in this field (Gumbiner, 1993).…”

Section: Molecular Bases For Tjmentioning

confidence: 99%

Tight junction-based epithelial microenvironment and cell proliferation

et al. 2008

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Section: Molecular Bases For Tjmentioning

confidence: 99%

Tight junction-based epithelial microenvironment and cell proliferation

et al. 2008

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“…It is generally accepted that these strands create the barrier. The biochemical composition of these strands is still a topic of investigation, but varied data support roles for both lipid and protein components (Furuse et al, 1998a(Furuse et al, , 1993Kachar and Reese, 1982;Meyer, 1983;Pinto da Silva and Kachar, 1982;Stevenson et al, 1986). Consistent with an important role of lipids, tight junction proteins are concentrated within cholesterol-enriched raftlike microdomains whose density -i.e.…”

Section: Tight Junction Compositionmentioning

confidence: 99%

The mucosal barrier at a glance

Turner

2017

Journal of Cell Science

190

183

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Mucosal barriers separate self from non-self and are essential for life. These barriers, which are the first line of defense against external pathogens, are formed by epithelial cells and the substances they secrete. Rather than an absolute barrier, epithelia at mucosal surfaces must allow selective paracellular flux that discriminates between solutes and water while preventing the passage of bacteria and toxins. In vertebrates, tight junctions seal the paracellular space; flux across the tight junction can occur through two distinct routes that differ in selectivity, capacity, molecular composition and regulation. Dysregulation of either pathway can accompany disease. A third, tight-junction-independent route that reflects epithelial damage can also contribute to barrier loss during disease. In this Cell Science at a Glance article and accompanying poster, we present current knowledge on the molecular components and pathways that establish this selectively permeable barrier and the interactions that lead to barrier dysfunction during disease.

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“…In freeze-fracture replicas, a complementary pattern of anastomosing strands and furrows is observed, which reflects the molecular organization of the junctional sites. The peculiarities of the strand structures and the membrane fusion images led Kachar, Pinto da Silva, and Reese to postulate that the strands of the tight junctions represent cylindrical, inverted lipid micelles [ 16,171. In this lipid model of the zonula occludens, the cytoplasmic leaflets of the apical and the basolateral plasma membrane domains are continuous, while the exoplasmic leaflets are interrupted by the hexagonal lipid cylinder.…”

Section: Tight Junctions Maintain Lipid Polaritymentioning

confidence: 99%

“…Very little is known about the molecular arrangement at the membrane contact sites of the tight junction. Models for the structure of the tight junction have been devised on the basis of its ultrastructural features [15][16][17]. At the site of the tight junction the intercellular space is occluded, and the two apposed plasma membranes seem to have fused or merged.…”

Section: Tight Junctions Maintain Lipid Polaritymentioning

confidence: 99%

Lipid polarity and sorting in epithelial cells

Meer

Simons

1988

J of Cellular Biochemistry

207

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Apical and basolateral membrane domains of epithelial cell plasma membranes possess unique lipid compositions. The tight junction, the structure separating the two domains, forms a diffusion barrier for membrane components and thereby prevents intermixing of the two sets of lipids. The barrier apparently resides in the outer, exoplasmic leaflet of the plasma membrane bilayer. First data are now available on the generation of these differences in Madin-Darby canine kidney (MDCK) cells, grown on filter supports. Experiments in which fluorescent precursors of apical lipids were introduced into the cell have demonstrated that upon biosynthesis apical lipids are sorted from basolateral lipids in an intracellular compartment. In this paper we present a model for the sorting process, the central point of which is that the two sets of lipids laterally segregate into microdomains that bud to form vesicles delivering the lipids to the apical and the basolateral plasma membrane domains, respectively.

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On tight-junction structure

Cited by 213 publications

References 34 publications

Tight junction-based epithelial microenvironment and cell proliferation

Tight junction-based epithelial microenvironment and cell proliferation

The mucosal barrier at a glance

Lipid polarity and sorting in epithelial cells

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