On the zopiclone enantioselective binding to human albumin and plasma proteins. An electrokinetic chromatography approach

Asensi-Bernardi, Lucía; Martı́n-Biosca, Yolanda; Medina-Hernández, M.J.; Sagrado, S.

doi:10.1016/j.chroma.2011.03.025

Cited by 12 publications

(13 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table 5 showed different studies that have been reported over the years for the evaluation of the binding affinity of HSA of numerous drugs and other bioactive compounds using CE techniques. Many studies focused on the individual binding parameters for each compound/enantiomer [153,155,161] while others also described competitive studies between compounds to the binding sites of HSA [156,157,159]. Furthermore, most of these studies were enantioselective studies where the differences in binding of each enantiomer of the analyzed compounds to HSA were evaluated and compared using, many of them, HSA as a chiral selector [150,152,154,155].…”

Section: Capillary Electrophoresismentioning

confidence: 99%

“…Furthermore, most of these studies were enantioselective studies where the differences in binding of each enantiomer of the analyzed compounds to HSA were evaluated and compared using, many of them, HSA as a chiral selector [150,152,154,155]. However, a few studies used cyclodextrins for this purpose while HSA was incubated with the samples before the analysis [151,153,161,162].…”

Section: Capillary Electrophoresismentioning

confidence: 99%

See 1 more Smart Citation

Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends

et al. 2021

View full text Add to dashboard Cite

The interaction between proteins and drugs or other bioactive compounds has been widely explored over the past years. Several methods for analysis of this phenomenon have been developed and improved. Nowadays, increasing attention is paid to innovative methods, such as high performance affinity liquid chromatography (HPALC) and affinity capillary electrophoresis (ACE), taking into account various advantages. Moreover, the development of separation methods for the analysis and resolution of chiral drugs has been an area of ongoing interest in analytical and medicinal chemistry research. In addition to bioaffinity binding studies, both HPALC and ACE al-low one to perform other type of analyses, namely, displacement studies and enantioseparation of racemic or enantiomeric mixtures. Actually, proteins used as chiral selectors in chromatographic and electrophoretic methods have unique enantioselective properties demonstrating suitability for the enantioseparation of a large variety of chiral drugs or other bioactive compounds. This review is mainly focused in chromatographic and electrophoretic methods using human serum albumin (HSA), the most abundant plasma protein, as chiral selector for binding affinity analysis and enantioresolution of drugs. For both analytical purposes, updated examples are presented to highlight recent applications and current trends.

show abstract

Section: Capillary Electrophoresismentioning

confidence: 99%

Section: Capillary Electrophoresismentioning

confidence: 99%

Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This method could help to differentiate between the patients with mild cognitive impairment who will progress to AD from those having a benign form of mild cognitive impairment without progression. The enantioselective binding of zopiclone by human serum albumin and total plasma proteins was also studied by chiral CE .…”

Section: Recent Applications Of Ce Enantioseparationsmentioning

confidence: 99%

Recent advances in electrodriven enantioseparations

Jáč

Scriba

2012

J of Separation Science

109

View full text Add to dashboard Cite

Capillary electromigration techniques have developed into significant analytical separation tools especially for enantioseparations. While CE can be considered a mature technique as documented by its wide applications, CEC is still in a developmental state despite many research efforts. The success of stereospecific CE separation methods is due to the high specificity and flexibility of the technique as well as the availability of many types of chiral selectors. Thus, numerous methods have been developed for the analysis of chiral compounds in chemical, biochemical, pharmaceutical, environmental, and forensic sciences. However, most reported applications deal with pharmaceuticals. The search for new chiral selectors also continued despite the fact that most applications were performed using cyclodextrins. Furthermore, CE has been combined with spectroscopic and molecular modeling studies in attempts to understand the interactions between chiral selectors and analytes. The present review focuses on recent examples of mechanistic aspects of capillary enantioseparations with regard to mathematical modeling of enantioseparations, investigations of the analyte-complex structures as well as new chiral selectors and applications of chiral analyses by CE and CEC. It covers the literature published between January 2011 and August 2012.

show abstract

“…Even though charged CDs were used initially for the enantioseparation of neutral compounds, they also have shown high enantioselectivity towards many basic and acidic compounds. It has been reported that maximum enantioselectivities are obtained when analytes and CDs have opposite mobilities (Asensi-Bernardi et al, 2011;Rudaz et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the enantioselective binding of imazalil to human serum albumin by capillary electrophoresis

Asensi-Bernardi

Martı́n-Biosca

Escuder-Gilabert

et al. 2015

Biomedical Chromatography

View full text Add to dashboard Cite

In this work, a methodology for the evaluation of enantioselective binding of imazalil (IMA) enantiomers to human serum albumin (HSA) that does not require the separation of free and bound to HSA fractions is developed. This methodology comprises the incubation of IMA-HSA designed mixtures for 30 min directly in the capillary electrophoresis system and the subsequent direct injection and chiral separation of IMA employing highly sulfated β-cyclodextrin as chiral selector and the complete filling technique. Two mathematical approaches were used to estimate apparent affinity constants (K1), protein binding and enantioselectivity (ES) for both enantiomers of IMA. Moderate enantioselective binding of IMA enantiomers to HSA (ES = 2.0) was shown by the 1:1 stoichiometry and log K1 values of 3.4 ± 0.4 and 3.1 ± 0.3 for the first and second eluted enantiomers, respectively.

show abstract

On the zopiclone enantioselective binding to human albumin and plasma proteins. An electrokinetic chromatography approach

Cited by 12 publications

References 34 publications

Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends

Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends

Recent advances in electrodriven enantioseparations

Evaluation of the enantioselective binding of imazalil to human serum albumin by capillary electrophoresis

Contact Info

Product

Resources

About