Based on critical examination of both fresh and dried specimens and a morphometric analysis of herbarium specimens we performed a comparative study of the three closely related Gastridium (Poaceae, Tribe: Poeae, Subtribe: Agrostidinae) species G. ventricosum, G. phleoides and G. scabrum collected in Italy. The study aimed to provide new diagnostic tools to improve the taxonomy of the genus and confirm species delimitation. Variation in floral morphology in the three species was explored using seven quantitative and ten qualitative characters measured on 318 spikelets, both awned and unawned. Statistical methods, including principal components analysis (PCA) and non‐metric multidimensional scaling (NMDS), were used. Numerical analyses showed a general distinctness of spikelets and florets, consistent with the three studied species, and revealed among and within species variation patterns in both spikelet types. Accordingly, the most informative quantitative characters appeared to be the width and length of glumes and the length of awns, useful to distinguish G. phleoides which has the narrowest glumes and longer awns than the other species, while G. scabrum has the widest glumes. The most informative qualitative characters appeared to be the presence/absence of thick hairs or minute tubercles on the upper side of the lemma. The autonomy of the three studied species was confirmed. The taxonomic significance of the results is briefly discussed, and notes on the species distribution are included. Some nomenclatural and taxonomic notes on G. scabrum, of which literature still showed evidence of a general unawareness, are provided.