On the true identity of <i>Sergentomyia gemmea</i> and description of a closely related species: <i>Se. raynali</i> n. sp

Depaquit, Jérôme; Vongphayloth, Khamsing; Siriyasatien, Padet; Polseela, Raxsina; Phumee, Atchara; Loyer, Mathieu; Vol, A.; Varlot, G.; Rahola, Nil; Brey, Paul T.; Sutherland, Ian W.; Hertz, Jeffrey C.; Léger, N.

doi:10.1111/mve.12393

Cited by 16 publications

(16 citation statements)

References 17 publications

(29 reference statements)

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“…hivernus (Raynal and Gaschen) and Se. khawi (Raynal) by using CytB -PCR [23,25]. In this study, the most abundant species in our samples was Se.…”

Section: Discussionmentioning

confidence: 92%

“…gemmea and Se. iyengari sand flies in Thailand may be misidentified based on morphology and molecular techniques [23,25]; moreover, all of the sequences of Se. iyengari deposited in GenBank (accession numbers MG770900, MG770901, MG770913, MG770914, MG770916–MG770918, MG770924, MG770926, and MG770927) and Se.…”

Section: Discussionmentioning

confidence: 99%

“…Each female sand fly was dissected under a stereomicroscope on a sterilized slide using sterile needles. The head and genitalia were mounted on slides with Hoyer’s medium for morphological character identification in all female sand flies, under a light microscope (Olympus, Tokyo, Japan), following the taxonomic keys described by Lewis (1978) [24], Phumee et al (2016) [23], and Depaquit et al (2019) [25]. The abdomen and the thorax of each female specimen were transferred to sterile 1.5 mL Eppendorf tubes with a lysis buffer for the detection of Leishmania and Trypanosoma species by the polymerase chain reaction (PCR).…”

Section: Methodsmentioning

confidence: 99%

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Detection of Leishmania and Trypanosoma DNA in Field-Caught Sand Flies from Endemic and Non-Endemic Areas of Leishmaniasis in Southern Thailand

Srisuton

Phumee

Sunantaraporn

et al. 2019

Insects

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Phlebotomine sand flies are tiny, hairy, blood-sucking nematoceran insects that feed on a wide range of hosts. They are known as a principal vector of parasites, responsible for human and animal leishmaniasis worldwide. In Thailand, human autochthonous leishmaniasis and trypanosomiasis have been reported. However, information on the vectors for Leishmania and Trypanosoma in the country is still limited. Therefore, this study aims to detect Leishmania and Trypanosoma DNA in field-caught sand flies from endemic areas (Songkhla and Phatthalung Provinces) and non-endemic area (Chumphon Province) of leishmaniasis. A total of 439 sand flies (220 females and 219 males) were collected. Head and genitalia dissection of female sandflies were done for morphology identification, and the remaining parts of those sand flies were then used for the detection of Leishmania and Trypanosoma parasites. The DNA was extracted from individual female sand flies. Polymerase chain reaction (PCR) anneal, specific to the ITS1 and SSU rRNA gene regions, was used to detect Leishmania and Trypanosoma DNA, respectively. The positive PCR products were cloned and sequenced. The results showed that the female sand fly species in this study consisted of Sergentomyia khawi (35.9%); Se. anodontis (23.6%); Phlebotomus betisi (18.6%); Ph. kiangsuensis (9.5%); Ph. asperulus (6.4%); Se. barraudi (2.3%); 0.9% of each Se. indica, Ph. stantoni, and Ph. major major; and 0.5% of each Se. sylvatica and Ph. mascomai. The PCR and sequence analysis were able to detect Leishmania and Trypanosoma DNA in sand fly samples, which were identified as L. martiniquensis, 1/220 (0.45%) in Se. khawi, 3/220 (1.36%) of T. noyesi in Se. anodontis, and Ph. asperulus. Fourteen (6.36%) of the unidentified trypanosome species in Se. khawi, Se. indica, Se. anodontis, Ph. asperulus, and Ph. betisi were found in all of the areas of this study. Interestingly, we found a 1/220 (0.45%) co-infection sample of L. martiniquensis and Trypanosoma in Se. khawi from Songkhla Province. These data indicate that several species of sand flies might be potential vectors of Leishmania and Trypanosoma parasites in southern Thailand. However, more extensive study for potential vectors using a larger number of sand flies should be conducted to prove whether these sand flies can be natural vectors of leishmaniasis and trypanosomiasis in both humans and animals. In addition, our study could be useful for the future study of infection prevention, including effective vector control for leishmaniasis and trypanosomiasis in Thailand.

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“…hivernus (Raynal and Gaschen) and Se. khawi (Raynal) by using CytB -PCR [23,25]. In this study, the most abundant species in our samples was Se.…”

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Leishmania and Trypanosoma DNA in Field-Caught Sand Flies from Endemic and Non-Endemic Areas of Leishmaniasis in Southern Thailand

Srisuton

Phumee

Sunantaraporn

et al. 2019

Insects

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“…Individual female sand flies were placed on a sterilized slide and dissected under a stereomicroscope equipped with an ice block, using 26G × 1/2″ sterile needles. Head and genitalia were mounted with Hoyer’s medium on a slide, which was later used for morphological species identification based on keys proposed by Lewis [ 19 ], Phumee et al [ 4 ], and Depaquit et al [ 20 ]. Molecular identification was performed to confirm the sand fly species using mitochondrial cytochrome b gene ( CytB )-PCR [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

A new cluster of rhabdovirus detected in field-caught sand flies (Diptera: Psychodidae: Phlebotominae) collected from southern Thailand

et al. 2021

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Background The distribution of phlebotomine sand flies is changing rapidly due to climate change. This issue has implications for the epidemiology of sand fly-borne diseases, especially sand fly-associated viruses. Few studies concerning sand fly-associated viruses have been conducted in Thailand. Therefore, this study aimed to perform a molecular survey of groups of pathogenic RNA viruses belonging to the Orbivirus, Phlebovirus, and Flavivirus genera and family Rhabdoviridae in sand fly samples collected from southern Thailand. Methods Sand flies were collected at two locations in Trang and Songkhla provinces of southern Thailand, and individual sand fly samples were processed for species identification and virus detection. The Orbivirus, Phlebovirus, and Flavivirus genera and family Rhabdoviridae molecular determination was performed by RT-PCR, and positive samples were identified by cloning and sequencing, cell culture inoculation, and phylogenetic analysis. Results The results presented in this study were based on the analysis of a total of 331 female sand flies. This molecular study revealed evidence of Rhabdoviridae family virus presence in Phlebotomus papatasi (3/331, 0.9%). The findings demonstrated a new cluster of rhabdovirus that was closely related to Bactrocera dorsalis sigmavirus strain BDSV.abc5 and the lineages of insect-specific Rhabdoviridae. In addition, the Bayesian tree suggested that the common ancestor of this group was the dimarhabdovirus clade. It was assumed that the virus may have switched hosts during its evolution. However, the detection of Orbivirus, Phlebovirus, and Flavivirus genera using specific primers for RT-PCR was negative in the collected sand flies. Conclusions There is limited knowledge on the genetic diversity and ecology of Rhabdoviridae in Thailand. This is the first data regarding the circulation of Rhabdoviridae in Ph. papatasi from Thailand. We found a new cluster of rhabdoviruses that was close to the new B. dorsalis sigmavirus. It is possible that there is a great deal of diversity in this family yet to be discovered, and a more extensive survey for new rhabdoviruses may uncover viruses from a wide diversity of host taxa and broaden our understanding of the relationships among the Rhabdoviridae. Graphical abstract

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“…The above examples underline that studying species complexes and eventually naming cryptic species is of practical interest. Examples are also available among Old World phlebotomine sand flies (e.g., Ilango, 2010; Gajapathy et al ., 2013; Kasap et al ., 2013; Depaquit et al ., 2019), as it is the case of Phlebotomus chabaudi Croset, Abonnenc & Rioux and Phlebotomus riouxi Depaquit, Léger & Killick‐Kendrick, putative vectors of Leishmania spp. (Lehrter et al ., 2017).…”

Section: Practical Implications Of Species Complexes and Cryptic Species Of New World Phlebotomine Sand Fliesmentioning

confidence: 99%

Beyond taxonomy: species complexes in New World phlebotomine sand flies

Sousa‐Paula

Pessoa

Otranto

et al. 2021

Medical Vet Entomology

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A species complex (= species group, species series) is an assemblage of species, which are related morphologically and phylogenetically. Recent research has revealed several arthropod vector species that were believed to be a single nominal species actually representing a group of closely related species, which are sometimes morphologically indistinguishable at one or more developmental stages. In some instances, differences in terms of vector competence, capacity, or both have been recorded. It highlights the importance of detecting and studying species complexes to improve our understanding of pathogen transmission patterns, which may be vectored more or less efficiently by different species within the complex. Considering more than 540 species, about one‐third of the phlebotomine sand flies in the New World present males and/or females morphologically indistinguishable to one or more species. Remarkably, several of these species may act in transmission of pathogenic agents. In this article, we review recent research on species complexes in phlebotomine sand flies from the Americas. Possible practical implications of recently acquired knowledge and future research needs are also discussed.

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On the true identity of Sergentomyia gemmea and description of a closely related species: Se. raynali n. sp

Cited by 16 publications

References 17 publications

Detection of Leishmania and Trypanosoma DNA in Field-Caught Sand Flies from Endemic and Non-Endemic Areas of Leishmaniasis in Southern Thailand

Detection of Leishmania and Trypanosoma DNA in Field-Caught Sand Flies from Endemic and Non-Endemic Areas of Leishmaniasis in Southern Thailand

A new cluster of rhabdovirus detected in field-caught sand flies (Diptera: Psychodidae: Phlebotominae) collected from southern Thailand

Beyond taxonomy: species complexes in New World phlebotomine sand flies

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