On the surface coat in the amphibian embryo

Løvtrup, S

doi:10.1002/jez.1401500304

Cited by 16 publications

(1 citation statement)

References 20 publications

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“…Dollander (9, I 1) observed an extracellular coat surrounding the fertilized egg of Triton which stained with Nile blue sulfate, but he was unable to demonstrate such a coat in later stages (10). LCvtrup (27) stated that an extracellular coat exists in Rana temporaria embryos which takes part in osmoregulation by mechanically opposing osmotic swelling. Bell (5,6) reported that a surface coat composed of mucopolysaccharides can be removed from Rana pipiens gastrulae and neurulae by means of focused ultrasound.…”

Section: Historymentioning

confidence: 99%

FINE STRUCTURE AND MORPHOGENIC MOVEMENTS IN THE GASTRULA OF THE TREEFROG, HYLA REGILLA

Baker

1965

The Journal of Cell Biology

112

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The blastoporal groove of the early gastrula of the treefrog, Hyla regilla, was examined with the electron microscope. The innermost extension of the groove is lined with invaginating flask-and wedge-shaped cells of entoderm and mesoderm. The distal surfaces of these cells bear microvilli which are underlain with an electron-opaque layer composed of fine granular material and fibrils. The dense layer and masses of vesicles proximal to it fill the necks of the cells. In flask cells bordering the forming archenteron the vesicles are replaced by large vacuoles surrounded by layers of membranes. The cells lining the groove are tightly joined at their distal ends in the region of the dense layer. Proximally, the cell bodies are separated by wide intercellular spaces. The cell body, which is migrating toward the interior of the gastrula, contains the nucleus plus other organelles and inclusions common to amphibian gastrular cells. A dense layer of granular material, vesicles, and membranes lies beneath the surface of the cell body and extends into pseudopodium-like processes and surface undulations which cross the intercellular spaces. A special mesodermal cell observed in the dorsal lining of the groove is smaller and denser than the surrounding presumptive chordamesodermal cells. A long finger of cytoplasm, filled with a dense layer, vesicles and membranes, extends from its distal surface along the edge of the groove, ending in a tight interlocking with another mesodermal cell. Some correlations between fine structure and the mechanics of gastrulation are discussed, and a theory of invagination is proposed, based on contraction and expansion of the dense layer and the tight junctions at distal cell surfaces.

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Section: Historymentioning

confidence: 99%

FINE STRUCTURE AND MORPHOGENIC MOVEMENTS IN THE GASTRULA OF THE TREEFROG, HYLA REGILLA

Baker

1965

The Journal of Cell Biology

112

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Osmoregulation in the amphibian egg the influence of calcium

Berntsson

Haglund

1965

J. Cell. Comp. Physiol.

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Determinations of the permeability coefficient for water show that the osmotic behavior of amphibian eggs (ovarian and coelomic eggs from Rana temporaria) cannot be accounted for by this factor alone. Comparison of the cortical stiffness of coelomic eggs with vitelline membrane i n iso-and hypotonic solutions indicate that a mechanical tension develops under hypotonic conditions, strong enough to resist osmotic swelling.In Ca++-free media preservation of this tension is interfered with, but the effect is observed only after exposure for some hours.C a + + does not change the diffusion coefficient for water i n the egg cytoplasm, nor the permeability coefficient for water through the cell membrane. Neither is the Young's modulus for the vitelline membrane influenced. A slight effect of C a + + on the cortical stiffness of naked coelomic eggs was observed. In hypotonic Ca++-free media no resistance to swelling develops in these eggs, and they disintegrate after a rather short time.To determine the permeability to water the rate of DzO -HzO exchange was measured with the Cartesian diver balance. The stiffness was determined with a cell elastimeter described by Mitchison and Swann, and the osmotic swelling was determined by measuring the egg diameter with a n eyepiece screw micrometer.

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Permeability changes in fertilized and activated amphibian eggs

Løvtrup

1962

J. Exp. Zool.

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On the surface coat in the amphibian embryo

Cited by 16 publications

References 20 publications

FINE STRUCTURE AND MORPHOGENIC MOVEMENTS IN THE GASTRULA OF THE TREEFROG, HYLA REGILLA

FINE STRUCTURE AND MORPHOGENIC MOVEMENTS IN THE GASTRULA OF THE TREEFROG, HYLA REGILLA

Osmoregulation in the amphibian egg the influence of calcium

Permeability changes in fertilized and activated amphibian eggs

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